Secondary herpes zoster wound cured by allogeneic platelet-rich plasma: a case report / 中国输血杂志

【Objective】 To investigate the feasibility of allogeneic platelet-rich plasma (PRP) for the treatment of herpes zoster wounds secondary to systemic lupus erythematosus (SLE), especially large ulcer wounds. 【Methods】 The treatment process of a patient with massive herpes zoster wounds in perineum and hip accompanied by extensive soft tissue necrosis secondary to SLE was retrospectively analyzed. The clinical efficacy of allogeneic PRP was explored combined with treatment key points and literature review. 【Results】 The patient′s wound bed was prepared until the wound was fresh, then treated externally with allogeneic PRP 3 times a week. The wound was healed completely after 42 days. 【Conclusion】 In the case of autologous PRP unavailable or unsuitable, allogeneic PRP is a safe alternative, which can effectively promote tissue regeneration, and this patient achieved curative effect in a short period of time.

To Explore the Effect of Xiangpi Shengji Ointment on the Apoptosis of Wound Cells in Model Rats after Anal Fistula Operation based on Fas/Fas L Pathway / 世界科学技术-中医药现代化

Objective To explore the effect of Xiangpi Shengji Ointment on wound healing and apoptosis-related Fas/Fas L pathway in model rats after anal fistula operation.Methods Thirty-six SD rats were used to construct the anal fistula model by using steel wire hanging line and indwelling for 30 days.After successful modeling,27 rats with anal fistula were randomly selected for"fistulectomy"to construct a postoperative wound model.After operation,the wound model rats were randomly divided into three groups,9 rats in each group,which were Xiangpi Shengji Ointment Group,Vaseline Group and Model Group,and the remaining 9 rats with anal fistula were sham operation group.The rats in the Xiangpi Shengji Ointment group were externally applied with Xiangpi Shengji Ointment gauze,while those in the Vaseline group were externally applied with Vaseline gauze.The rats in the model group were only disinfected and rinsed.No special treatment was given to the rats in the sham operation group.The wound healing was observed on the 3rd,5th,7th and 10th day after medication intervention,and the wound healing rate was calculated.After 10 days of continuous intervention,wound tissues were taken from each group,and the histopathological changes,the number of apoptosis,the expressions of Fas,Fas L,caspase-8 and cyto-c in wound tissues were observed by HE staining,TUNEL staining and immunohistochemistry respectively,and the mRNA expressions of Fas,Fas L and cyto-c in wound tissues were detected by RT-PCR.Results Compared with the model group,Xiangpi Shengji ointment group and Vaseline group significantly promoted wound healing at 7 and 10 days after intervention(P<0.01),and the wound healing rate of Xiangpi Shengji ointment group was significantly higher than that of Vaseline group(P<0.01).After 10 days of drug intervention,compared with sham operation group,the apoptosis rate of Xiangpi Shengji ointment group,Vaseline group and model group increased significantly(P<0.01),and the relative expressions of Fas,Fas L and cyto-c mRNA and the expression levels of Fas,Fas L,caspase-8 and cyto-c protein in wound tissue increased significantly(P<0.05,P<0.01).Compared with the model group,the apoptosis rate,the relative expression of Fas,Fas L and cyto-c mRNA and the expression level of Fas,Fas L,caspase-8 and cyto-c protein in Xiangpi Shengji ointment and Vaseline groups decreased significantly(P<0.05,P<0.01).Compared with Vaseline group,the apoptosis rate of Xiangpi Shengji ointment group decreased significantly(P<0.01),and the relative expression of Fas,Fas L and cyto-c mRNA and the expression level of Fas,Fas L,caspase-8 and cyto-c protein decreased significantly(P<0.05,P<0.01).Conclusion Xiangpi Shengji Ointment can inhibit the activation of Fas/Fas L pathway,reduce the apoptosis of wound tissue cells and promote wound healing after anal fistula operation.

Clinical observation on wound measurement grid and perforator flap design / 中华显微外科杂志

Objective:To investigate the application of self-designed wound measuring grid in the measurement of wound and the design of perforator flap.Methods:From February 2018 to February 2020, 31 cases of soft tissue defects of limbs were repaired by free transfer of perforator flap. During the operation, the self-designed membrane with measurement grid was used to measure the wound surface. The flaps were designed and harvested according to the accurate measurement of the wound. The flaps were reviewed at hospital clinic during follow-up.Results:All patients entered 3-15 months of follow-up with an average of 8 months. All 31 flaps survived, of which 2 flaps encountered vascular crisis and were rescued after surgical exploration. There was no infection of flaps and all flaps had stage I healing. The flaps were satisfactory in appearance with the sensation recovery to S 2 in 21 cases and S 3 in 10 cases. Only linear scars were left on the donor sites. Conclusion:The membrane with a measurement grid can accurately measure a wound, and help the design of the flap according to the measured size and profile of the wound. It can reduce the operation time and the risk as well as to improve the operation efficiency. The clinical application is satisfactory. Large scale and multi-centre studies are required to further prove the benefit of the measurement grid.

Progress in application of wound negative pressure therapy in flap repair / 中华整形外科杂志

Flap is a main way to repair complex wound defects, however its related complications cannot be ignored. The wound negative pressure therapy (NPWT) can help to promote angiogenesis and granulation, absorb tissue exudate, inhibit bacterial proliferation. It also help with flap survival, saving crisis flaps, and accelerating wound healing of donor site. Therefore, the NPWT is widely used in tissue repairment and reconstruction. This article reviewed and summarized the mechanism of NPWT, as well as its application in the donor and recipient site of flap.

Repair of complex foot and ankle wounds with bilateral anterolateral thigh flaps / 中华整形外科杂志

Objective@#To explore the clinical effect of bilateral anterolateral thigh flaps in repairing complex wounds of foot and ankle.@*Methods@#From April 2014 to June 2017, 16 patients with complex foot and ankle wounds were treated in Ruihua Hospital Affiliated to Soochow University. There were 15 males and 1 female, aged from 12 to 73 years. Two of them had two wounds for each, the area of which was from 4 cm ×5 cm to 6 cm×10 cm. Fourteen cases were single-wounds, the area of which ranged from 11 cm×8 cm to 42 cm×15 cm. According to the wound surfaces, the wide wound cloth was divided into two pieces of cloth with a width less than 8 cm. The double-leaf skin flap was designed at the point of the perforating branch of the skin flap located by B-ultrasound on the iliac-patellar connecting line of the donor area. The course of the origin artery of the skin perforating branch of the skin flap was observed by DSA image. If the perforating branch of the double-leaf skin flaps was the common origin artery, the two adjacent wounds would be repaired directly. If the perforator of the double-leaf skin flaps is not the common artery, the double-leaf skin flaps were cut separately. After the pedicle division, the perforator vessels of the high perforator skin flaps were anastomosed with the distal end of the medial circumflex lateral femoral artery of the low perforator skin flaps to form a new parallel double-leaf skin flaps, which were then assembled or lobed to the recipient area. Flap donor sites were sutured directly.@*Results@#11 cases were repaired with parallel bilateral anterolateral thigh flaps with double perforators, and 5 cases were repaired with new parallel bilateral thigh flaps formed by anastomotic bridging of perforator vessels. The area of the flaps ranged from 5 cm×6 cm to 8 cm×22 cm. All the 16 flaps survived and the donor site wounds healed in one stage. Five cases underwent internal fixation removal and skin flap thinning 6 to 10 months after operation. Follow-up for 6 to 18 months showed that the skin flaps were of good color and texture, and the sensation of the flaps ranged from S1 to S3. Linear scars were left in all donor sites and lower limb movements were normal.@*Conclusions@#It is a good method to repair complex foot and ankle wounds with bilateral anterolateral thigh flaps, and it has the advantages of flexible design, less damage to donor site and good blood supply of the flap.

Individualized design for arteriae circumflexa femoris lateralis descending branch of perforator flap and ecological protection with flap-supply area / 中华整形外科杂志

Objective@#To summarize the individual design of the perforator flap based on the descending branch of the lateral circumflex femoral artery and ecological protection of the flap.@*Methods@#From June 2013 to June 2017, 33 cases of extremities wound defects were repaired with the descending branch perforator flap of the lateral circumflex femoral artery. According to the chracteristics of extremity wound and the anatomy of the descending branch perforator of the lateral circum flex artery, a medial incision of the flap according to the demarcation line was performed. Through meticulous dissection, two perforator branches were found and traced on the surface of facia lata. The size of the kiss flap was adjusted according to the perforator caliber. The chimeric muscular flap was incised according to muscular penetration point of the perforator and the course of lateral fermoral nerve with cautious protection of muscular branch of femoral nerve. During the incision, fascia lata, nerves and muscles on donor site were protected to reduce the damage to donor site. Meanwhile, ecological structure on donor site was reconstructed. Our series include 22 males, 11 females. The average age ranged from 13 to 71 years (mean: 47years). There were 21 cases of wounds on dorsum manus and wrists and 12 cases of wounds on the dorsum pedis and lower limbs. The wound dimension ranged from 7 cm×8 cm to 13 cm×24 cm. The flap dimension ranged from 6 cm×7 cm to 8 cm×15 cm.There were 22 cases of KISS flaps and 11 cases of chimeric flaps. Wounds on all donor sites were primarily sutured. Course of the disease ranged from 1 week to 2 months.@*Results@#All flaps were harvested uneventfully and survived well. No vascular crisis occurred. Wounds on all donor sites were primarily sutured. Three to 24 (mean 18) months of follow-up was made on 28 cases. The color and texture of flaps was good. Appearance of the flaps was good. Protective sensation recovered. Only linear scar can be seen on donor sites. No muscular hernia and cinesipathy was noticed on donor sites.@*Conclusions@#The size of KISS flaps depends on the caliber of perforator branch estimated intraoperatively. Muscular part of the chimeric flaps was harvested according to the muscular entry points of perforators and the demand of recipient sites. The wound defects were reconstructed individually according to the peculiarity of the defects. Fascialate, nervus cutaneus and muscle was cautiously protected in the course of flap harvest. Ecological structure was reestablished after flap harvest to reduce complications.

Effects of Two Different Hemostatic Methods of Electric Coagulation or Suture Hemostasisa on Ovarian Reservation Function After Laparoscopic Ovarian Surgery / 中国微创外科杂志

Objective To evaluate the effects of electric coagulation or suture hemostasis on ovarian reservation function in laparoscopic ovarian cyst removal for ovarian endometriomas. Methods A total of 118 patients receiving laparoscopic ovarian cystectomy in our hospital from January 2011 to June 2015 were enrolled in this study.According to the habit of different operators,the patient were devided into either electric coagulation group(n =53)or suture group(n =65).The follicle-stimulating hormone (FSH),luteinizing hormone(LH),estradiol(E2)and antral follicle count(AFC)of the two groups were observed and compared before surgery and one and six months after surgery. Results There were no differences in the levels of the FSH,LH,E2and AFC between the two groups before the surgery(P>0.05).The level of FSH was increased and those of E 2and AFC were declined in both groups at 1 month after the operation compared with those before operation(P <0.05).Moreover, there were more significant differences in FSH,E2,and AFC in the electric coagulation group(P <0.05).There was no significant difference in LH(P>0.05).The levels of FSH and E 2recovered in both groups at 6 months after operation.There was no difference in FSH and E 2 compared with the level before operation in the suture group(P>0.05), however, there was significant difference in the electric coagulation group(P<0.05). Conclusions The laparoscopic ovarian cystectomy for ovarian endometriomas leads to decreased ovarian reserve.As compared to bipolar coagulation, suture hemostasis can effectively reduce the damage of ovarian function, which should be applied as possible as we can.

Effects of Two Different Hemostatic Methods of Electric Coagulation or Suture Hemostasisa on Ovarian Reservation Function After Laparoscopic Ovarian Surgery / 中国微创外科杂志

Objective To evaluate the effects of electric coagulation or suture hemostasis on ovarian reservation function in laparoscopic ovarian cyst removal for ovarian endometriomas. Methods A total of 118 patients receiving laparoscopic ovarian cystectomy in our hospital from January 2011 to June 2015 were enrolled in this study.According to the habit of different operators,the patient were devided into either electric coagulation group(n =53)or suture group(n =65).The follicle-stimulating hormone (FSH),luteinizing hormone(LH),estradiol(E2)and antral follicle count(AFC)of the two groups were observed and compared before surgery and one and six months after surgery. Results There were no differences in the levels of the FSH,LH,E2and AFC between the two groups before the surgery(P>0.05).The level of FSH was increased and those of E 2and AFC were declined in both groups at 1 month after the operation compared with those before operation(P <0.05).Moreover, there were more significant differences in FSH,E2,and AFC in the electric coagulation group(P <0.05).There was no significant difference in LH(P>0.05).The levels of FSH and E 2recovered in both groups at 6 months after operation.There was no difference in FSH and E 2 compared with the level before operation in the suture group(P>0.05), however, there was significant difference in the electric coagulation group(P<0.05). Conclusions The laparoscopic ovarian cystectomy for ovarian endometriomas leads to decreased ovarian reserve.As compared to bipolar coagulation, suture hemostasis can effectively reduce the damage of ovarian function, which should be applied as possible as we can.

The Change of Surface Temperature in Healing Process of Wound and Burn in Porcine Model

PURPOSE: The goal of wound managements are to prevent further tissue injury during dressing changes, and absorb the excessive exudates, provide moist condition, reduce the infection. But the usual dressing methods have several limitations to serve the information about wound surface environment. Moist, pressure, temperature, pH are the important factors that give us the information about the healing process phase, speed of healing, & the risk of infection. METHODS: We made full thickness wounds & burn on porcine model, then assessed the surface temperature intermittently until repithelialization were completed, and size measurement were done with Image Pro Plus 6.0. RESULTS: In wound areas, the surface temperature was lowest at post operation #1 day, then slightly increased till POD #21 days, and again down slope curvature. In burn area, the surface temperature was highest at POD #1 day, then slightly decreased till POD # 21 days, and more fall curvature. Modern sensing technology along with wireless radio frequency communication technology is posed to make significant advances in wound management. Our result will be a basic data for the future researches about the surface temperature monitor to detect the early infection and the intervention to modulate the surface temperature to increase the rate of epithelialization. CONCLUSION: The result of the correlation between the surface temperature and area reduction have weak or moderate negative Pearson correlation coefficient.

Reparation of large coloboma raw surface after facial tumour resection in elderly patients / 中华老年医学杂志

Objective To investigate the surgical reparation of large coloboma raw surface after facial tumour resection in elderly patients.Methods According to the position and characteristics of tumor as well as the age and tolerance of the patients, full thick skin graft, the skin flaps with subcutaneous pedicle and free skin flap were designed and used in the reparation.Results 24 cases with large coloboma raw surface (5 cm×7 cm-12 cm× 16 cm)were treated by the utilization of three approaches after tumor resection.The large coloboma raw surface in all patients achieved the healing with satisfactory appearance.Conclusions After facial tumour resection, the large coloboma raw surface can be repaired by using the skin graft, skin flaps after tumor resection or free skin flap if designed reasonably.The procedure of operation is simple and the therapeutic effect is satisfactory.

Effect observation of photon therapeutic apparatus irradiation applied in the postoperative wound healing about ischial tuberosity cyst / 重庆医学

Objective To observe the efficacy of the photon therapeutic apparatus on wound healing .Methods Totally 60 patients who had received the operation for their ischial tuberosity cysts were randomly divided into observation group and control group ,30 cases each in each group .The control group received conventional dressing change .The observation group received con-ventional treatment on the basis of the application of photon therapeutic apparatus for adjuvant therapy ,2 times a day ,7 days a course of treatment .Both of groups had the same other treatment and nursing care .The clinical efficacy of the two groups was ob-served .Results After the 4 and 7 days of treatment ,the observation group was superior to the control group on pain level ,content of extravasate(P< 0 .05) .The healing time of observation group was less than control group(P< 0 .01) .Conclusion The photon therapy is effective in the treatment of wound healing of patients with ischial tuberosity cysts after operation .

Effect of comprehensive nursing on small area of diabetic foot ulcer / 中国实用护理杂志

Objective To study comprehensive nursing meassures for small area of diabetic foot ulcer. Methods A total of 58 patients with small area of diabetic foot ulcer in our department were divided into two groups.The comprehensive nursing group (CN group,30 cases) adopted comprehensive nursing and treatment as follows, surgical dcbridement, infrared radiation of wound surface,sprinkling of growth factors(bFGF and rhEGF),covered by hydrocolloid dressing(Algoplaque).The control group(28cases) received hydropathic compress with 0.5% povidone iodine or 5% furacillin solution.Wound heal-ing progreasion,average healing time and the positive rate of wound germiculture of the two groups were observed. Results The average healing time of the CN group was (29.6±9.4)d,which was shorter than that of the control group,( 39.2±13.5 )d, P < 0.05. Statistical difference existed in the positive rate of wound germiculture at the 7th day and the 14th day between the two groups (P < 0.05). Conclusions Routine medical treatment and general nursing measures plus surgical debridement,infrared radiation of wound sur-face,sprinkling of growth factors and hydrocolloid dressing possess facilitative healing effect for small area of diabetic foot ulcer.

Clinical Applications of Modified Superficial Sural Fasciocutaneous Island Flap

In case of soft-tissue defects with bone and tendon exposure on foot, ankle and lower leg, it is often impossible to achieve wound closure by a simple skin graft. The sural artery flap is a fasciocutaneous flap supplied by the sural artery that accompanies the sural nerve and connects with a septocutaneous perforator of the peroneal artery via a suprafascial network of vessels. For the coverage of the defects, we operated 30 cases using modified superficial sural fasciocutaneous island flap based on the proximal or distal. and we covered convoluted wound site with this flap with plicated fascia. We reconstruct complicated skin defects on the foot region(13) and the distal portion of the leg(17). The size of flap varied from 3 x 4 cm2 to 10 x 9 cm2. All 30 flaps survived completely, but minor complications, such as venous congestion, hematoma disappeared after a few days. The main advantage of this flap is a constant and reliable blood supply without sacrifice of a major artery. but disadvantage of this flap is hypoesthesia at the lateral part of the foot. In conclusion, dissection of the superficial sural fasciocutaneous island flap is quite easy and requires less time, and involves less risk to the patient. The nonbulky fasciocutaneous island flap appearance particularly indicated small to medium sized defects with or without convoluted wound surface. The contours of the recipient and donor sites are acceptable aesthetically.

JOLLYCOME~(TM) Irrigation on Incision and Wound Surface:A Clinical Report / 中华医院感染学杂志

OBJECTIVE To compare the effect of antibacterial irrigation in clinic.METHODS(JOLLYCOME~(TM)) was used to irrigate operative wounds for 17 206 patients in departments of general surgery,gynecotokology,pediatric surgery,(urology,) burn,orthopedics,cardiothoracic surgery,otorhinolaryngology and stomatology.(RESULTS) All operative incisions were with grade A healing.Rate of instantaneous sterilization of(JOLLYCOME~(TM)) on operative wound and mucocutaneous surface was 100% and it was failed to detection any bacteria on operative wound 24h after operation.CONCLUSIONS JOLLYCOME~(TM) irrigation could provide the effect of continuous sterilization on operative wound before suturing and play an important positive role in control(infection) in hospital.