Thinking of Traditional Chinese Medicine in Prevention and Treatment of Panvascular Diseases Based on ''Blood Vessel-Zangfu Organ-Syndrome Differentiation and Treatment'' / 中国实验方剂学杂志

The term ''panvascular'' refers to the human vascular system， which is a complex network of arteries， veins， and lymphatic vessels. Panvascular diseases refer to a group of vascular system diseases， with vascular atherosclerosis as the common pathological feature. The panvascular diseases in target organs such as the heart， brain， kidney， and limbs are caused by ischemia or bleeding， including arterial system diseases， venous system diseases， microcirculation system diseases， and Zangfu organ-blood vessel diseases. The concept of panvascular diseases integrates vascular lesions and target organ damage. In clinical practice， blood vessels in multiple regions are regarded as a large vascular unit system， and vascular lesions and the induced target organ damage are considered as a whole. Based on the holistic concept and the Zangxiang theory in traditional Chinese medicine （TCM）， the ''blood vessel-Zangfu organ-syndrome differentiation and treatment'' network is built， on the basis of which a pattern of vascular disease-Zangfu organ dysfunction-syndrome differentiation and treatment is applied to the TCM diagnosis and treatment of panvascular diseases. The theory of treating arterial system diseases from the heart， venous system diseases from the kidneys， and microvascular system diseases from the liver is proposed. According to the causes identified based on syndrome differentiation， this paper summarizes the methods of reinforcing Yang and activating blood （including warming Yang and activating blood， replenishing Qi and activating blood， replenishing Qi， nourishing Yin and activating blood， activating Yang and blood， dispersing cold and activating blood）， cooling blood and resolving stasis， tonifying kidney and promoting urination coupled with activating blood and dredging vessels， nourishing Yin and tonifying kidney coupled with activating blood and dredging vessels， and soothing liver and regulating Qi coupled with activating blood and dredging collaterals， as well as wind-extinguishing medicines， applied to the treatment of panvascular diseases， aiming to provide methods and ideas for the treatment of vascular diseases with TCM.

Effect of electroacupuncture at the acupoints for Tiaozang Xingshen on cerebral metabolism in the patients with type 2 diabetes mellitus-associated cognitive dysfunction / 中国针灸

OBJECTIVES@#To investigate the cerebral metabolism in the patients with type 2 diabetes mellitus-associated cognitive dysfunction (T2DACD) and explore the mechanism of electroacupuncture (EA) at the acupoints for Tiaozang Xingshen (adjusting zangfu function and rescuing the spirit) in treatment of T2DACD, using magnetic resonance spectroscopy.@*METHODS@#Fifteen patients with T2DACD (observation group) and 22 healthy subjects (control group) were enrolled. In the observation group, the patients were treated with EA for Tiaozang Xingshen at Baihui (GV 20) and Shenting (GV 24), and bilateral Feishu (BL 13), Pishu (BL 20), Shenshu (BL 23), Zusanli (ST 36), Sanyinjiao (SP 6), Hegu (LI 4) and Taichong (LR 3). EA was operated with disperse-dense wave, 2 Hz/100 Hz in frequency and 0.1 mA to 1.0 mA in current intensity; 30 min each time, once daily. One course of EA consisted of 5 treatments, at the interval of 2 days and the intervention lasted 8 courses. Before treatment in the control group, before and after treatment in the observation group, the score of Montreal cognitive assessment scale (MoCA), the score of clinical dementia rating (CDR), Flanker paradigm, Stroop paradigm, Nback paradigm, the score of self-rating anxiety scale (SAS), the score of self-rating depression scale (SDS), and the score of Hamilton depression rating scale (HAMD) were evaluated separately; the glycolipid metabolic indexes (fasting plasma glucose [FPG], glycosylated hemoglobin type A1c [HbA1c], total cholesterol [TC], triacylglycerol [TG], high-density lipoprotein cholesterol [HDL-C] and low-density lipoprotein cholesterol [LDL-C]) were determined；with the magnetic resonance spectroscopy technique adopted, the metabolites in the basal ganglia area were detected. The correlation analysis was performed for the metabolite values with MoCA score, CDR score , Flanker paradigm, Stroop paradigm, and Nback paradigm.@*RESULTS@#Before treatment, compared with the control group, in the observation group, MoCA score was lower (P<0.001), CDR score and the levels of FPG and HbA1c were higher (P<0.001); the reaction times of Flanker non-conflict, Flanker conflict, Stroop neutrality, Stroop congruence, Stroop conflict, and 1-back were prolonged (P<0.05, P<0.001), and the accuracy of Flanker conflict, Stroop conflict, and 1-back decreased (P<0.05, P<0.01); the ratio of N-acetyl aspartate (NAA) to creatine (Cr) in the left basal ganglia area was dropped (P<0.001), and that of myo-inositol (MI) to Cr in the right side increased (P<0.05). In the observation group after treatment, compared with the levels before treatment, MoCA score was higher (P<0.001), the scores of CDR, SAS and HAMD were reduced (P<0.01, P<0.05), the reaction times of Flanker conflict and Stroop conflict shortened (P<0.001, P<0.05), and the accuracy of Flanker conflict and 1-back increased (P<0.001, P<0.05); the ratio of NAA to Cr in the left basal ganglia area and that of the gamma-aminobutyric acid (GABA) to Cr in the right increased (P<0.05), that of MI to Cr in the right decreased (P<0.05). Before treatment, in the observation group, the ratio of MI to Cr in the right basal ganglia area was positively correlated with the reaction time of Stroop congruence (r=0.671, P=0.012) and this ratio was positively correlated with the reaction time of Stroop conflict (r=0.576, P=0.039).@*CONCLUSIONS@#Electroacupuncture for "adjusting zangfu function and rescuing the mind" improves the cognitive function of T2DACD patients, which may be related to the regulation of NAA, MI and GABA levels in the basal ganglia.

Exploration of the diagnosis and treatment for benign prostatic hyperplasia with acupuncture based on jingjin theory / 中国针灸

Benign prostatic hyperplasia is caused by kidney deficiency and impaired qi transformation of the urinary bladder and is manifested by the stagnation of essence chamber. Based on jingjin (muscle region of meridian, sinew/fascia) theory and taking the visceral membrane as the principal, acupuncture is delivered at sinew/fascia to promote qi circulation, resolve stasis and open the orifice. Guided by CT, the needle is inserted at Zhongji (CV 3), the front-mu point of the urinary bladder, and then goes to the prostatic capsule, meaning "the disease of zang organ is treated by needling the front-mu point". In treatment of benign prostatic hyperplasia, this acupuncture therapy stimulates the different layers of fascia, by which, the defensive qi on the exterior is regulated and "essence orifice" in the interior is adjusted so that the urination can be promoted.

One View of the Establishment of the Relationship Between the Zang-Fu and the Meridians in the Huangdi Neijing Suwen / 全日本鍼灸学会雑誌

[Objective] The Zang-Fu and the meridians, which are one of the foundations of modern acupuncture and moxibustion medical education, appeared in "Lingshu" in the "Meridian" section. However, the Zang-Fu and the meridians each has a history of independent development in ancient China Before the Common Era. The purpose of this study is to investigate the process by which the meridians came to be associated with the Zang-Fu, drawing from descriptions in "Suwen", which was compiled Before the Common Era.[Method] The source literature of this study is "Revisions and Extensive Notes on the Huangdi Neijing Suwen", with reference to "Acupuncture and Moxibustion Medicine and Classic Research" by MARUYAMA Masao to classify compilations of "Suwen". The research items are the names of the Zang and the Fu, the relationships between the Zang and the Fu, and the piao-li relationships of the Zang-Fu and the three Yin and three Yang of the hands and feet. [Result] The names for the Zang were Liver, Heart, Spleen, Lungs and Kidneys, and for the Fu were Small Intestine, Bladder, Gallbladder, Large Intestine, Stomach, and Sanjiao. The Zang-Fu relationships were Lungs-Large Intestine, Spleen-Stomach, Heart-Small Intestine, Kidney-Bladder, Liver-Gall Bladder, and Chronic Illness-Sanjiao. The piao-li relationships of the Zang-Fu and the three Yin and three Yang of the hands and feet were Foot Thai-Yang and Shao-Yin, Foot Shao-Yang and Chueh-Yin, Foot Yang-Ming and Thai-Yin, Hand Thai-Yang and Shao-Yin, Hand Yang-Ming and Thai-Yin, and Hand Shao-Yang and Heart. The Liver is treated by Foot Chueh-Yin and Shao-Yang, the Heart is treated Hand Shao-Yin and Thai-Yang, the Spleen is treated by Foot Thai-Yin and Yang-Ming, the Lungs are treated by Hand Thai-Yin and Yang-Ming, and the Kidneys are treated by Foot Shao-Ying and Thai-Yang. There was the expression "太陰陽明表裏為脾胃脈也" that Hand-Foot, Yin-Yang and the Zang-Fu were grouped by their Piao-li relationships. And there were the expressions saying "Shao-Yin is the Kidneys" and "Yang-Ming is the Stomach", although these were not in complete form. [Discussion] The Zang-Fu meridians, combining the Zang-Fu and the meridians, started from the Spleen-Stomach. There were other expressions combining the Zang-Fu with the meridians, although these were also not in complete form. While medicine at that time was being organized and integrated with the Yin-Yang and Five Elements theories, the relationships between the Zang-Fu and the meridians were also established. And it is inferred that the process of combining these and developing them into a new medical system existed during this period. [Conclusion] The relationships between the Zang-Fu and the meridians were established in "Suwen", which was compiled Before the Common Era, and the expression combining them started from "太陰陽明表裏為脾胃脈也".

Clinical study of acupuncture using the principle of invigorating viscera and purging fu, replenishing qi and nourishing yin for the type 2 diabetes mellitus patients with qi and yin deficiency / 国际中医中药杂志

Objective:To evaluate the effect of acupuncture with invigorating viscera and purging fu, replenishing qi and nourishing yin for the T2DM patients with qi and yin deficiency syndrome.Methods:A total of 59 patients who met the inclusion criteria from January 1, 2019 to December 31, 2020 in the Endocrinology Department of the Second Affiliated Hospital of Anhui University of Chinese Medicine were divided into the acupuncture group with 30 cases and a control group with 29 cases, according to the random number table method. The control group received conventional hypoglycemic treatment. And the acupuncture group was treated with acupuncture on the basis of the control group treatment. Both groups were treated for 4 weeks. TCM syndrome scores were performed before and after treatment, respectively. Serum IL-6 was measured by ELISA, serum CRP by immunoturbidimetry, and serum fasting blood glucose (FPG), 2 hPG, HbAlc, TC, TG, HDL-C, LDL-C levels by spectrophotometry. The BMI, clinical effect rates were calculated.Results:The total effective rate was 96.7% (29/30) in acupuncture group and 75.9% (22/29) in control group, and the difference was statistically significant ( χ2=5.96, P<0.01). After treatment, the scores of fatigue, dryness of mouth and pharynx, spontaneous sweating and night sweating, shortness of breath and lazy speech in acupuncture group were significantly lower than those in the control group ( t values were 6.02, 4.31, 4.34, 3.63, respectively, all Ps<0.01). The serum CRP level in acupuncture group was significantly lower than that of the control group at 21, 48 d after treatment ( t values were -4.36, -3.75, respectively, all Ps<0.01), and IL-6 level was significantly lower than that of the control group at 14, 21, 28 d after treatment ( t values were -2.92, -5.35, -8.71, respectively, all Ps<0.01). After treatment, the serum TC [(4.62±0.68) mmol/L vs. (5.56±0.72) mmol/L, t=5.16], TG [(1.48±0.42) mmol/L vs. (2.12±0.89) mmol/L, t=3.55], LDL-C [(2.48±0.84) mmol/L vs.(3.02±0.95) mmol/L, t=2.32] in the acupuncture group were significantly lower than those in the control group ( P<0.05), HDL-C [(1.39±0.27) mmol/L vs. (1.26±0.22) mmol/L, t=-2.02] in the acupuncture group was significantly higher than that of the control group ( P<0.05). The serum FPG level at 21 d [(6.12±0.67) mmol/L vs. (6.57±0.61) mmol/L, t=-4.96], 28 d [(5.78±0.52) mmol/L vs. (6.49±0.58) mmol/L, t=-2.70] in acupuncture group were significantly lower than those in the control group ( P<0.01). The 2h PG level at 14 d after treatment [(10.23±1.06) mmol/L vs. (11.76±1.34) mmol/L, t=-4.87], 21 d [(9.05±0.98) mmol/L vs. (10.53±1.24) mmol/L, t=-5.10], 28 d [(7.45±0.69) mmol/L vs. (9.31±0.78) mmol/L, t=-9.71] in the acupuncture group were significantly lower than those in the control group ( P<0.01), and HbA1c level were decreased 14 d [(7.93±0.86)% vs. (8.52±0.97)%, t=-2.47], 21 d [(7.63±0.85)% vs. (8.15±0.92)%, t=-2.26], 28 d [(6.47±0.51)% vs. (7.51±0.62)%, t=-7.05] significantly lower than those in the control group ( P<0.01), BMI [(22.13±1.57) kg/m 2vs. (24.16±1.82) kg/m 2, t=-4.59] 28 d after treatment was significantly lower than that of the control group ( P<0.01). Conclusion:Acupuncture therapy of nourishing viscera and purging fu, nourishing qi and nourishing yin can regulate the disorder of glucose and lipid metabolism in patients with T2DM and syndrome of qi and yin deficiency.

Effects of Nardostachys jatamansi on gut microbiota of rats with Parkinson's disease / 中国中药杂志

Under the guidance of the traditional Chinese medicine(TCM) theory of "Zangfu-organs of spleen and stomach" and the modern theory of "microbiota-gut-brain axis", this study explored the effects of Nardostachys jatamansi on the gut microbiota of rats with Parkinson's disease(PD). The 40 SD rats were randomly divided into the control group, PD model group, levodopa group, and Nardostachys jatamansi ethanol extract group. The PD model was established by subcutaneous injection of rotenone in the neck and back area. After 14 days of intragastric administration, the PD rats' behaviors were analyzed through open field test, inclined plane test, and pole test. After the behavioral tests, the striatum, colon, and colon contents of rats in each group were collected. Western blot was employed to detect the protein expression of tyrosine hydroxylase(TH) and α-synuclein(α-syn) in striatum and that of α-syn in colon. Enzyme linked immunosorbent assay(ELISA) was used to detect the levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and nuclear factor-kappa B(NF-κB) in striatum and colon. High-throughput sequencing of 16 S rRNA gene was conducted to detect the differences in microbial diversity, abundance, differential phyla, and dominant bacteria of rats between groups. The results indicated that Nar. ethanol extract could relieve dyskinesia, reverse the increased levels of α-syn, TNF-α, IL-1β, and NF-κB in striatum, and improve the protein expression of TH in striatum of PD rats. The α diversity analysis indicated a significant decrease in diversity and abundance of gut microbiota in the PD model. The results of linear discriminant analysis effect size(LEfSe) of dominant bacteria indicated that Nardostachys jatamansi ethanol extract increased the relative abundance of Clotridiaceae, Lachnospiraceae, and Anaerostipes, and reversed the increased relative abundance of Proteobacteria, Gammaproteobacteria, Enterobacteriaceae, and Escherichia-Shigella in PD model group to exhibit the neuroprotective effect. In summary, the results indicated that Nar. ethanol extract exert the therapeutic effect on PD rats. Specifically, the extract may regulate gut microbiota, decrease the levels of proinflammatory cytokines, and reduce the protein aggregation of α-syn in the colon and striatum to alleviate intestinal inflammation and neuroinflammation. This study provides a basis for combining the theory of "Zangfu-organs of spleen and stomach" with the theory of "microbiota-gut-brain axis" to treat PD.

Professor -' clinical experiences of "syndrome differentiation and Chinese herbal plaster application" based on meridian and theories / 中国针灸

Focusing on the limits of the plaster application of Chinese herbal medicine in clinical practice, i.e. limit in the region exerted, inadequate meridian effect, more attention to the exterior rather than the interior and limit in the application of differentiation, professor - proposes his clinical experiences in the plaster application of Chinese herbal medicine based on meridian and differentiation, including: The Chinese herbal plaster application alone meridian course guided by meridian differentiation. Multidimensional plaster application on the base of the meridian differentiation of muscle region. Split-field plaster application by taking collateral differentiation as principle. Selective plaster application in association with disease differentiation. Specific plaster application rooted on differentiation.

Discussion on the academic system of "theory, method, recipe and degree" in s infantile Chinese massage of the western part of Hunan province / 中国针灸

The theory and technique of s infantile Chinese massage of the western part of Hunan province are reviewed in clinical diagnosis and treatment and the core theoretical system is refined. Based on clinical experiences and in consideration of the analysis on relevant literature, the academic system of s infantile Chinese massage of the western part of Hunan province is summarized as four aspects, named "theory, method, recipe and degree". "Theory" refers to the four diagnostic methods and eight principle in terms of diagnosis and treatment and the therapeutic emphasis on the main disorders of organs in children. "Method" refers to promotion and restriction of five elements, meridian tropism in treatment. "Recipe" refers to the selection of acupoints, optimization of therapeutic techniques and the key in the composition of the herbs with different roles considered, named monarch herb, minister herb, assistant herb and guiding herb. "Degree" refers to the strength and intensity of manipulation to ensure the optimal effect. All of the aforementioned reviews provide the reference to theoretic study on this Chinese massage school.

Cultural factors in the theories of meridians and organs / 中国针灸

It takes quite a long process in the formation of the relevant theories of meridians and organs and many cultural factors are involved in the development of these theories at different eras, e.g. "the five evolution and six climatic factors", "twelve as the representing number of universe", "twenty-eight stars", "three and three " and "ancient bureaucratic system". Some of the theories are not coincident with the clinical practice at present. By the influence of the doctrine as "the five evolution and six climatic factors", only eleven meridians were recorded in the early ancient literature. Due to the saying "twelve as the representing number of universe", twelve meridians were developed and the other related meridians classified into the categories as the collaterals and the extraordinary meridians. Because of the theory of three and three , it is understandable why the indications of the clinical practices are not correlated with the relevant theories of meridians and organs. For example, the indications of three meridians of hand are not related to the large intestine, the small intestine and , and the corresponding acupoints in the treatment for the related disorders are located in the lower extremities. The ancient bureaucratic system had affected the understanding of the organs functions. For example, the heart is regarded as the monarch, protected by the pericardium. The hand- meridian-related cardiac disorders match with the pericardium. Hence, the cardiac disorders are treated by the acupoints of the hand- meridian rather than the hand- meridian. In this paper, the cultural factors in the theories of meridians and organs are deeply analyzed and those factors that are not coincident with the present clinical practice are separated. The analytic results are significant to the future research on meridians and organs.

Clinical study on self-made Jianpi-Tiaogan-Wenshen decoction in the treatment of diarrhea-predominant irritable bowel syndrome / 国际中医中药杂志

Objective@#To evaluate the efficacy of self-made Jianpi-Tiaogan-Wenshen decoction in the treatment of diarrhea-predominant irritable bowel syndrome (IBS).@*Methods@#A total of 107 patients with diarrhea- predominant IBS who met the inclusion criteria were divided into control group (53 cases) and observation group (54 cases) by random number table method. On the basis of routine treatment, the control group was given Bifidobacterium triple viable powder and trimebutine maleate dispersible tablets, while the observation group was given self-made Jianpi-Tiaogan-Wenshen decoction. Both groups were treated for 4 weeks. The symptoms and signs were scored before and after treatment. The level of 5-hydroxytryptamine (5-HT) was measured by double antibody sandwich method. The mast cell count was observed under high power microscopy, and the clinical efficacy was evaluated.@*Results@#The total effective rate was 90.7% (49/54) in the observation group and 60.4% (32/53)in the control group, and there was significant difference between the two groups (χ2=13.406, P<0.01). After treatment, the levels of mast cell count (10.03 ± 2.19 pcs/Hp vs. 14.85 ± 1.98 pcs/Hp, t=11.935), 5-HT (7.06 ±1.37 mg/L vs. 9.67 ± 1.31 mg/L, t=10.069) significantly decreased in the observation group (P<0.05). After treatment, the VAS score, stool frequency score and stool character score of the observation group were significantly lower than those of the control group (t=11.560, 11.957, 20.539, respectively, P<0.05).@*Conclusions@#Self-made Jianpi-Tiaogan-Wenshen decoction can improve clinical symptomsof diarrhea-predominant IBS patients, reduce mast cell count and 5-HT level, and improve the clinical efficacy.

Advances in Modern Research on the Liver Function in Traditional Chinese Medicine and its Association with Other Organs / 世界科学技术-中医药现代化

The liver in the traditional Chinese medicine (TCM) theory of visceral manifestation has rich connotations. Thestructure and function of liver differed from that of liver in modern recognition. Research on TCM theory has partiallyexplained the liver theory. The function of controlling dispersion of liver was associated with neuro-endocrine-immunesystem, liver sinus endothelial function, and metallothionein, etc. While the activity of storing blood of liver related withblood coagulation factors, anticoagulant and so on. The functional interaction between Zang-organ and Fu-organ maycorrelate with the lung-liver axis and liver-intestine axis. The review summarized the modern explanation on liverfunction in TCM and its relationship with Fu-organs to help the development of TCM basic theory in liver function.

Investigating the Pathogenetic Development of Alzheimer's Disease from the Perspective of "Transformation of Zang-fu Meridians" / 世界科学技术-中医药现代化

It is great value to analyze the pathogenesis of Alzheimer's disease for the formulation and research of prevention and treatment strategies. The change of zang-fu meridians and collaterals is the essential rule of the pathogenesis of internal injury and miscellaneous diseases. Based on the analysis of the physiological basis of the aging changes of zang-fu meridians and the relationship between dementia and the abnormal function of zang-fu meridians and collaterals, this paper systematically explored the relationship between the changes of zang-fu meridians and the pathogenesis of dementia. It is considered that the change of zang-fu meridians and collaterals is the basic law of its pathogenesis development. Therefore, it is proposed that strengthening Qi is the essential principle throughout the whole process of dementia, and dredging channels and collaterals and maintaining normal function of meridians and collaterals are the key to block the pathogenesis evolution of dementia.

Relationship Between Distribution of Oral Ulcer and Zang-fu Viscera Syndrome Differentiation / 广州中医药大学学报

Objective To study the relationship between the distribution of oral ulcer and the syndrome differentiation of Zang-fu viscera. Methods We collected the data of 65 oral ulcer patients admitted in Stomatology Department,the First Affiliated Hospital of Guangzhou University of Chinese Medicine from January of 2015 to July of 2016. The data of emotion,diet,sleep,history of digestive tract disease,and family history of the patients with tongue ulcer and the patients with labio-buccal ulcer were compared. The relationship of the distribution of labio-buccal ulcer,tongue-tip ulcer,and ulcer of lateral part of the tongue with Zang-fu viscera syndrome differentiation was analyzed by statistical software SAS 9.2. Results(1)Tongue ulcer group had higher incidence of anxiety and higher percentage of history of digestive tract disease than labio-buccal ulcer group(P<0.01,P<0.05). (2)Labio-buccal ulcer mostly had a correlation with spleen deficiency blended with dampness or gastrointestinal accumulating heat, the tongue-tip ulcer mostly had a correlation with heart-fire flaring up, and the ulcer of lateral part of tongue mostly had a correlation with pathogenic fire derived from liver-qi stagnation, the difference among the 3 groups being statistically significant(P < 0.01). Conclusion The distribution of oral ulcer has a correlation with Zang-fu viscera syndrome differentiation.

Textual research on origin and development of Zang-Fu syndrome differentiation / 国际中医中药杂志

Zang-fu syndrome differentiation from Huangdi Neijing, laid the theoretical foundation. Hua Tuo'S Zhongzhang Jing, Sun Simiao's Qianjin-Yaofang, Zhang Yuansu Yixue-Qiyuan and other ancient physicians enriched the Zang-fu theory.Zang-fu syndrome differentiation became systematic and widely used in clinical. This paper traced the source of the theory, explored the development of syndrome differentiation theory.

Acupuncture-moxibustion syndrome differentiation based on differences betweendiseases anddiseases / 中国针灸

The clinical syndrome differentiation system of acupuncture-moxibustion was proposed in this paper, which should reflect the core of acupuncture theory and characteristics of treatment, and take syndrome differentiation of meridian as key component. In case of meridian syndrome differentiation guided by syndrome differentiation of eight principles, extra emphasis should be placed on the differences of-diseases anddiseases. Differentiating location should be key in-diseases; the acupoints should be selected according to location, and appropriate technique should be applied according to symptoms.diseases should based on syndrome differentiation of meridian and assisted by syndrome differentiation of; the key ofdiseases treatment was meridian-based acupoint selection, assisted by location-based acupoint selection; the emphasis should be placed on special acupoints, and reinforcing and reducing technique was selected according to cold-heat and deficiency-excess.

Analysis on property of meridian supramolecules by biological evolution path / 中国针灸

With human placed in the whole nature, by following the biologic evolution path, the property of channel structure for "imprinting template" in meridian andwas explored with supramolecular chemistry. In the history of biologic evolution, each molecule in "molecule society" gradually developed into various highly-ordered supramolecular bodies based on self-identification, self-assembly, self-organization, self-replicating of"imprinting template", and thereby the original biochemical system was established, and finally evolved into human. In the forming process of supramolecular bodies, the channel structure of"imprinting template" in guest supramolecular bodies would be kept by host supramolecular bodies, and communicate with the outside to exchange materials, energy, information, otherwise life phenomenon could not continue, for which it was the chemical nature of biolo-gical supramolecular bodies for body to develop meridian. Therefore, the human was a gigantic and complicated supramolecules body in biological nature, and possessed the supramolecules "imprinting template" at each stage of evolution, for which the meridians were formed. When meridians converged, acupoints appeared; when acupointsconverged,appeared. With the promotion of the blood from heart, according to"imprinting template", the guest supramolecular bodies and host meridian produced-analysis, which was the-phenomenon of guest in meridian. It presented asimage of physiology and pathology as well as action regularities of medication and acupuncture tolerance, by which current various meridian viewpoints could be explained and propose the hypothesis of meridian supramolecular bodies. The meridian and its phenomenon was decide by its "imprinting template" of supramolecular bodies and self-reaction regularities, which abided through the living nature. This was the substance for meridian biology.

Analysis on Five Kinds of Tone and Meridian Therapy Thoughts / 世界科学技术-中医药现代化

The Yellow Emperor's Canon of Internal Medicine first introduced the Five-tone Therapeutic System into traditional Chinese medicine (TCM).The five-tone meridian therapy is based on the theory of yin-yang,five elements,meridians,acupoint theory,and other methods to complement each other.It is a comprehensive prevention,diagnosis and treatment of physical and mental diseases of TCM.This therapy combines the pentameter and its internal organs with meridians.It is a profound experience and summary of the resonant interaction between music and emotional and visceral organs of the sound frequency.Doctor differentiates the disease based on sound variant lesions,and then treats by corresponding meridians,acupoints,so as to achieve the treatment purpose.

Discussion on specificity of acupoint effect based on "acupoint fordiseases" / 中国针灸

The specificity of acupoint effect is an important part of acupuncture theory. It has been one of the foci in acupuncture research domestically and abroad. This paper explores the specific relationship between Zusanli (ST 36) and stomach-intestine function based on the ancient records on "acupoint fordiseases" and the clinical and experimental researches on Zusanli(ST 36) treating digestive diseases. The physiological mechanism of the specificity is explained in terms of the spinal cord segment of Zusanli (ST 36) and gastrointestinal organs as well as the cerebral central effect of puncturing the acupoint. The aim of the paper is to provide more evidences for the specificity of acupoint effect indicated by "acupoint fordiseases".

Mr. Wang Xugao's Treating Infants Diseases / 浙江中医药大学学报

[Objective] To sum up Mr. Wang Xugao's experience in treating infants diseases. [Method] By analysis on infants medical cases treated by Mr. Wang Xugao such as fetus scare, child tuberculosis, Severe Malnutrition, acute asthmatic attack in children,etc., sum up his features in treating such dis-eases. [Result] In treating infants diseases, he pays attention to differentiation and analysis of organs deficiency and excessiveness, very according with the gist of Mr. Qian Yi's theory of infants physiologytender organs, un-enough Qi bloodand pathology easy deficiency and excessiveness, easy cold and hot. [Conclusion] Mr. Wang's treatment on infants diseases is flexible and changeable, with good cure effect.

Effect of Acupuncture of "Taixi" (KI 3) on Protein Expression in the Renal Tissues of Healthy Rats / 针刺研究

Objective To observe the changes of renal protein expression after electroacupuncture (EA) of "Taixi" (KI 3) of the Kidney Meridian of Foot-Shaoyin in healthy rats so as to explore the relationship between KI 3 and the Kidney. Methods Twelve adult male Wistar rats were randomly divided into normal control group (n=6) and EA group (n=6). EA (2 Hz/100 Hz,2-4 mA) was applied to bilateral KI 3 for 20 min,once a day for 7 days. Under anesthesia,the rats were perfused transcardiacally with ice saline and their kidneys removed for extracting the total proteins and assaying the differentially expressed proteins with two-dimensional gel electrophoresis (2-DE),Image MasterTM 2 D Platinum Software and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) MALDI-TOF-MS,respectively. Results Image MasterTM 2 D Platinum Software analysis showed that after EA,9 protein points of the kidney tissues expressed differentially above 3-fold in comparison with control group were upregulated,2 of which were identified to be NAD-dependent isocitrate dehydrogenase and Quinone reductase. No downregulated differentially expressed proteins were found in the kidney tissues. Conclusion EA of "Taixi" (KI 3) can increase the expression of NAD-dependent isocitrate dehydrogenase and Quinone reductase in the kidney tissue,suggesting an increase of energy metabolism after EA and a close correlation between the KI 3 (Source-point) and the Kidney.