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1.
J. appl. oral sci ; 26: e20170084, 2018. graf
Artigo em Inglês | LILACS, BBO | ID: biblio-893718

RESUMO

ABSTRACT Objective: This study aimed to evaluate bone repair in rat dental sockets after implanting nanostructured carbonated hydroxyapatite/sodium alginate (CHA) and nanostructured carbonated hydroxyapatite/sodium alginate containing 5% strontium microspheres (SrCHA) as bone substitute materials. Methods: Twenty male Wistar rats were randomly divided into two experimental groups: CHA and SrCHA (n=5/period/group). After one and 6 weeks of extraction of the right maxillary central incisor and biomaterial implantation, 5 μm bone blocks were obtained for histomorphometric evaluation. The parameters evaluated were remaining biomaterial, loose connective tissue and newly formed bone in a standard area. Statistical analysis was performed by Mann-Withney and and Wilcoxon tests at 95% level of significance. Results: The histomorphometric results showed that the microspheres showed similar fragmentation and bio-absorbation (p>0.05). We observed the formation of new bones in both groups during the same experimental periods; however, the new bone formation differed significantly between the weeks 1 and 6 (p=0.0039) in both groups. Conclusion: The CHA and SrCHA biomaterials were biocompatible, osteoconductive and bioabsorbable, indicating their great potential for clinical use as bone substitutes.


Assuntos
Animais , Masculino , Estrôncio/farmacologia , Regeneração Óssea/efeitos dos fármacos , Carbonatos/farmacologia , Durapatita/farmacologia , Substitutos Ósseos/farmacologia , Alvéolo Dental/efeitos dos fármacos , Nanoestruturas/uso terapêutico , Alginatos/farmacologia , Osteogênese/efeitos dos fármacos , Osteogênese/fisiologia , Estrôncio/química , Fatores de Tempo , Regeneração Óssea/fisiologia , Carbonatos/química , Distribuição Aleatória , Reprodutibilidade dos Testes , Transplante Ósseo/métodos , Resultado do Tratamento , Ratos Wistar , Espectroscopia de Infravermelho com Transformada de Fourier , Durapatita/química , Substitutos Ósseos/química , Alvéolo Dental/fisiologia , Ácido Glucurônico/farmacologia , Ácido Glucurônico/química , Nanoestruturas/química , Alginatos/química , Ácidos Hexurônicos/farmacologia , Ácidos Hexurônicos/química
2.
J. appl. oral sci ; 23(6): 599-608, Nov.-Dec. 2015. graf
Artigo em Inglês | LILACS, BBO | ID: lil-769812

RESUMO

ABSTRACT Objective The aim of this study was to investigate the in vitro and in vivo biological responses to nanostructured carbonated hydroxyapatite/calcium alginate (CHA) microspheres used for alveolar bone repair, compared to sintered hydroxyapatite (HA). Material and Methods The maxillary central incisors of 45 Wistar rats were extracted, and the dental sockets were filled with HA, CHA, and blood clot (control group) (n=5/period/group). After 7, 21 and 42 days, the samples of bone with the biomaterials were obtained for histological and histomorphometric analysis, and the plasma levels of RANKL and OPG were determined via immunoassay. Statistical analysis was performed by Two-Way ANOVA with post-hoc Tukey test at 95% level of significance. Results The CHA and HA microspheres were cytocompatible with both human and murine cells on an in vitro assay. Histological analysis showed the time-dependent increase of newly formed bone in control group characterized by an intense osteoblast activity. In HA and CHA groups, the presence of a slight granulation reaction around the spheres was observed after seven days, which was reduced by the 42nd day. A considerable amount of newly formed bone was observed surrounding the CHA spheres and the biomaterials particles at 42-day time point compared with HA. Histomorphometric analysis showed a significant increase of newly formed bone in CHA group compared with HA after 21 and 42 days from surgery, moreover, CHA showed almost 2-fold greater biosorption than HA at 42 days (two-way ANOVA, p<0.05) indicating greater biosorption. An increase in the RANKL/OPG ratio was observed in the CHA group on the 7th day. Conclusion CHA spheres were osteoconductive and presented earlier biosorption, inducing early increases in the levels of proteins involved in resorption.


Assuntos
Humanos , Animais , Masculino , Alginatos/farmacologia , Materiais Biocompatíveis/farmacologia , Regeneração Óssea/efeitos dos fármacos , Durapatita/farmacologia , Nanoestruturas/uso terapêutico , Contagem de Células , Ácido Glucurônico/farmacologia , Ácidos Hexurônicos/farmacologia , Teste de Materiais , Osteoblastos/efeitos dos fármacos , Osteoprotegerina/sangue , Ratos Wistar , Receptor Ativador de Fator Nuclear kappa-B/sangue , Reprodutibilidade dos Testes , Fatores de Tempo , Alvéolo Dental/efeitos dos fármacos , Difração de Raios X
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