Simultaneous determination of preservatives [sodium benzoate and potassium sorbate] in soft drinks and herbal extracts using high-performance liquid chromatography [HPLC]

Chemical preservation has become an increasingly important practice in modern food technology and herbal medicinal products with the increase in production of processed and convenience products. In the present study, a reversed-phased HPLC method for simultaneous determination of the preservatives sodium benzoate [SB] and potassium sorbate [PS] is described. The separation of SB and PS were performed on the C[18-] column and acetonitrile -ammonium acetate buffer as mobile phase. The detector wavelength was set at 254 nm. Separation of the two components [SB and PB] was achieved in less than 6 min. Analytical characteristics of the separation such as limit of detection, limit of quantification, accuracy, precision and reproducibility were evaluated. The range of preservatives found were from not detected [nd] - 2477 mg 1[-1], nd - 328 mg 1[-1] for SB, PS respectively. In This study, has shown that the concentration of SB and PS in the soft drink samples is higher that ADI even for normal consumers, based on maximum limits specified in national standards and on model diets

High performance liquid chromatogaphic determination of mixtures of benzoic acid and sorbic acid and methyl paraben and propyl paraben in some soft drinks and pharmaceutical formulations

A rapid, specific and accurate reversed phase HPLC method was developed and validated for the determination of cited compounds in bulk powder samples and commercial pharmaceutical and food products. HPLC was performed with a Pecosphere C18 column "Perkin-Elmer" [33 x 4.6 mm, 3 mum] with a mobile phase of methanol: water [60: 40] for the determination of the two parabens with UV detection at lambda 255 nm and methanol-phosphate buffer pH 45 [8: 92] for the determination of the two acids with UV detection at lambda 235 nm. The influence of pH of phosphate buffer and its ratio in mobile phase was discussed. The typical linear range extended from 1-10 mug/ml for methyl paraben, 1-12 mug/ml for propylparaben, 5-50 mug/ml for sorbic acid and 5-100 mug/ml for benzoic acid. The results were evaluated by a linear regression analysis

Acidos grasos esenciales en la producción actual de oleaginosas de la República Argentina: posibilidades futuras de diversificación / Essential fatty acids in the present vegetable oils production of the República Argentina: future possibiities in the diversification trends production

Se presenta un resumen de los logros alcanzados durante casi 50 años en un tema fundamental del conocimiento de las características de composición acídica de aceites vegetales de producción masiva en la república Argentina. Su desarrollo, en parte coincidente con la evolución del conocimiento analítico, ha sido de utilidad a la nutrición, normalización, legislación alimentaria, tecnología, uso y contralor de aceites vegetales y de pulpa de frutos. El progreso observado en los avances de modernas técnicas de la Biotecnología tiende a una apertura en la diversificación de la producción