Determination of ethyl glucuronide in blood and urine by LC-MS/MS / 法医学杂志

OBJECTIVE@#To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).@*METHODS@#After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.@*RESULTS@#Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.@*CONCLUSION@#The method is capable for blood and urine EtG analysis.

Vantagens e limitacoes do uso da urina como amostra biologica na dosagem alcoolica / Advantages and limitations in the use of urine as biological sample in the alcoholic dosage

Tendo em vista o grande interesse do uso da urina nos programas de controle e prevencao do uso de alcool e drogas no ambiente de trabalho e nas clinicas de reabilitacao, surge a necessidade de estudos mais detalhados sobre o fator de conversao utilizado para tranformar os valores de concentracao urinaria e sanguinea e sua validade...

Consumo reciente de alcohol y drogas en accidentes del trabajo / Recent use of alcohol and drugs in victims of work related accidents

Background: Twenty to twenty two percent of work related accidents have a direct or indirect relationship with recent alcohol use. Aim: To detect recent alcohol or drug use in patients who required hospitalization for severe work related accidents. Patients and methods: Blood and urine samples were obtained from patients admitted to a hospital within 6 hours of an accident severe enough to require hospitalization. Blood alcohol and urinary metabolites of cocaine, marijuana, amphetamines and benzodiazepines were measured. Patient identity was kept confidential throughout the study. Once patients were stabilized, they were interviewed by a psychologist that was blind to laboratory results. Results: Two hundred thirty men and 18 women were studied and 29 percent (30 percent of men and 17 percent of women) had recent use of alcohol or drugs. The most common substances were alcohol in 15 percent and benzodiazepines in 13 percent. Use of alcohol or drugs was found in 21 percent of professionals, in 33 percent of other occupational levels, half of agricultural workers and 25 percent of commerce workers. Only two of 60 positive cases, admitted substance use during the interview. Most of negative cases admitted the use of alcohol or other drugs during the month prior to the accident. Conclusions: Recent use of alcohol and drugs is frequent among severe work related accident victims. Probably, a high proportion of these subjects are problem drinkers. The combination of alcohol and benzodiazepines is an additional risk factor

Modificaciones de los niveles de acido delta aminolevulico dehidrasa en pacientes alcoholicos cronicos / Changes in the levels of delta-aminolevulinic acid dehydratase in chronic alcoholic patients

Estudios clínicos y bioquímicos fueron realizados sobre 58 pacientes con alcoholismo crónico a su vez divididos en 3 grupos: A (hepatopatia alcohólica), B (hepatopatía y neuropatía alcohólica) y C (neuropatía alcohólica). Simultáneamente se investigaron varios parámetros relacionados con la biosíntesis del hemo. Se comprobó que el ácido delta-aminolevúlico (ALA), porfobilinógeno (PBG) urinarios y las porfirinas urinarias y fecales, no presentaron diferencias significativas entre los grupos estudiados. También se determinaron en eritrocitos las actividades de ALA-dehidrasa (ALA-D), uroporfirinógeno-I-sintetasa y uroporfirinógeno-III-sintetasa. De todos los datos obtenidos se comprobó que solamente el ALA-dehidrasa en los grupos B y C se hallaba significativamente disminuida (P < 0,002). La disminución de los niveles de ALA-D se correspondió con la severidad de la neuropatía.