In vivo effects of Faizol Ubat Batuk, a herbal product on aminopyrine metabolism in rat hepatocytes

Traditional medicines, in particular herbal products, have been used abundantly over the years in curing several diseases. Pharmacological interactions of herbal products with modern drugs, however, remain to some extent unknown. Herein, we examined whether co-administration of Faizol Ubat Batuk [FUB], a mixture of aqueous extract of different plants, modifies the metabolism of aminopyrine, a conventional analgesic drug, in rat liver. We used rat hepatocytes outfitted by collagenase perfusion technique. Determination of aminopyrine n-demethylase activity was performed using the Nash colorimetric method, by measuring the amount of formaldehyde produced. Compared to control treatment, FUB significantly increased the hepatic metabolism of aminopyrine in healthy adult male rats. In contrast, the hepatic metabolism of aminopyrine in adult female rats was decreased. Besides, a biphasic effect in n-demethylase activity was observed in young male rats treated with FUB. In a subsequent experiment, FUB did not change the metabolism of aminopyrine in streptozotocin [STZ]-diabetic adult male rats. In conclusion, administration of FUB could affect phase I aminopyrine metabolism in rat heptocytes. In addition, the effects of FUB on hepatic n-demethylase activity were gender and disease dependent

Evaluación del metabolismo hepático de drogas en la diabetes experimental / Drug hepatic metabolism evaluation in experimental diabetes

Se desarrolló un modelo de diabetes experimental en ratas , analizaron los parámetros fisiológicos, el contenido de citocromo p-450 hepático y el metabolismo in vitro Fase I y II de estructuras moleculares modelos. Se observaron alteraciones significativas en el contenido de proteína microsomal hepática en las ratas diabéticas en tanto el tratamiento con insulina a estas ratas determinó una disminución del contenido de citocromo p-450 hepático. Las ratas diabéticas presentaron una capacidad metabólica in vitro Fase 1 mayor que las ratas controles y la administración de insulina produjo diversos efectos según sustrato empleado. El metabolismo in vitro Fase II de las ratas diabéticas resultó similar al obtenido en las ratas controles, en cambio el tratamiento con insulina produjo variaciones en la afinidad de la enzima por el sustrato y en la cantidad de la enzima que cataliza la reacción. Los resultados sugieren que el estado de diabetes y su tratamiento con insulina, modifica en forma diferente las isoenzimas citocromo P-450 y reduce selectivamente algunas enzimas UDP glucuronil transferasas.