Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry

A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specically sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95% of the test results were interpreted as positive. The limits of detection in liver tissue were 0.35 microgram/ml for furazolidone, 0.70 microgram/ml for sulfamethazine and 7.80 microgram/ ml for amprolium. In kidney tissues, they were 0.30 microgram/ml for furazolidone, 0.54 microgram/ml for sulfamethazine, and 7.6 microgram/ml for amprolium. It was concluded that this tube test could be used to screen for the residue of these three drugs in poultry.

Simultaneous determination of sulphaquinoxaline and amprolium in veterinary mixture by HPLC and first derivative ultraviolet spectrophotometry

Two simple and reliable methods are described for the determination of sulphaquinoxaline [SQX] and amprolium [AMP] combined in veterinary powder. The first method depends on high performance liquid chromatography on a reversed phase column [micro. Bondapak-C[18]] using mobile phase consisting of acetonitrile-methanol-water-phosphoric acid [30: 5: 55: 0.7, by volumes] at a flow rate of 1 ml min[-1] with UV detection at 254 nm. The detection limits were 0.2 micro,g ml[1] and 0.35 micro g ml' and linearity ranges were 2-20].micro g ml and 4-40[.micro g ml for SQX and AMP, respectively. The second one deals with the first derivative ['D] spectrophotometry with zero-crossing measurment at 253 nm and 226 nm for SQX and AMP respectively. The detection limits were 0.3 micro.g ml and 1.2 micro g ml[1] and linearity ranges were 1 -18 micro,g ml1 and 4-32 micro.g ml1 for SQX and AMP, respectively, the proposed procedures were successfully applied to the determination of these drugs in laboratory prepared mixtures with mean percentage recovery ranged from 98.50 to 99.75 and in commercial preparation ranged from 103.85 to 104.85