Novel inflammatory biomarkers in acute coronary syndrome

No abstract available.

Evaluation of total-LDH / heat resistant- LDH ratio in patients with acute myocardial infarction and unstable pectoral angina

Measurement of serum enzymes levels, such as lactate dehydrogenaze [LDH] and its isoenzymes is a routine way for diagnosis of myocardial infarction. One of these isoenzymes is LDH-1 which is raised after myocardial infarction. The purpose of this study was to evaluate total-LDH/heat resistant-LDH ratio in patients with acute myocardial infarction and unstable pectoral angina. In an descriptive analitical study 220 patients with suspected myocardial infarction [MI] or unstable pectoral angina [UA], hospitalized in CCU ward of Ekbatan Hospital in Hamedan city were studied. Using WHO's criteria, 110 were classified as having myocardial infarction and 110 as having unstable pectoral angina. The serum level of total-LDH and heat resistant-LDH was measured by NADH exchange method, 24 to 48 hours after their admission to the hospital. Data were analyzed by student-t test and Mann-Witheny test. Our study showed that total-LDH / heat resistant-LDH ratio in MI patients was 1.27 +/- 0.18 and it was 2.51 +/- 1.39 in UA patients [P < 0.001]. In our study, we found that when total LDH/ heat resistant-LDH ratio is decreased the severity of myocardial infarction [number of involved leads] and creatine phosphokinase [CPK] is increased [P < 0.01]. We did not find any relationship between total LDH/ heat resistant-LDH ratio with sex, age, type of MI [Q-wave and Non Q-wave], block after MI and arrhythmia following MI. Our results revealed that measuring heat resistant- LDH can be used as a method for accurate diagnosis of MI. This simple method can be used in centers where other accurate tests are not available

Study of leukocytic hydrolytic enzymes in patients with acute stage of coronary heart disease.

BACKGROUND: Coronary heart disease (CHD) is a major killer worldwide. Atherosclerosis, which is the basis of CHD, is believed to be an inflammatory disorder. Though various aspects of atherosclerosis are extensively studied, leukocytic hydrolytic enzymes are not studied very well with respect to CHD. AIM: This study was planned to assess changes associated with leukocytic hydrolases in CHD patients. SETTING AND DESIGN: A tertiary care hospital; case-control study. MATERIALS AND METHODS: 106 patients with acute myocardial infarction, 60 patients with unstable angina and 45 healthy controls were included in the study. Acid phosphatase, lysozyme, adenosine deaminase (ADA) and cathepsin-G levels were estimated from leukocytes. Reduced glutathione (GSH) and malondialdehyde (MDA) levels were measured. STATISTICAL ANALYSIS: Statistical comparison of data was done using student's t-test (unpaired). Correlation difference was calculated by using Pearson correlation coefficient. RESULTS: Significantly higher levels of acid phosphatase, lysozyme, ADA with lower levels of cathepsin G in leukocytes were observed in CHD group. We also found significantly higher levels of serum MDA with lower concentrations of blood GSH in CHD group. In diabetic CHD group, significantly higher levels of leukocytic acid phosphatase, lysozyme, ADA and serum MDA with lower levels of cathepsin G and blood GSH were observed. CONCLUSIONS: Our study indicates that leukocyte hydrolytic enzymes, mainly acid phosphatase, lysozyme and ADA were more active in CHD patients and may contribute to inflammation related with CHD. Its also indicates that leukocyte cathepsin-G may have antiinflammatory role.