RESUMO
A enxertia alógena de nervo teve seu interesse renovado após o desenvolvimento de melhores drogas imunossupressoras. Neste trabalho estudou-se a enxertia alógena de nervo utilizando a técnica de planimetria por contagem de pontos.Foram considerados três grupos: Grupo A - ratos Lewis que receberam enxertos de nervo de doadores isogênicos; Grupo B - ratos Lewis que receberam enxertos de nervo de ratos doadores Brown-Norway e foram tratados com solução salina; Grupo C - ratos Lewis que receberam enxertos de nervo de ratos doadores Brown-Norway e foram tratados com ciclosporina. A regeneração neural foi avaliada por análise histológica e estudos histomorfométricos depois de 6 e 12 semanas. Com 6 semanas, a densidade de fibras neurais e a porcentagem de tecido neural no grupo de enxertos alógenos com imunossupressão (grupo C) era significativamente mais alta do que no grupo B. Os grupos de enxertos alógenos (grupo B e C) mostraram densidade menor de fibras de nervo e porcentagem de tecido neural que no grupo de enxerto autógeno (grupo A) tanto com 6 quanto com 12 semanas.O método de planimetria por contagem de pontos produziu resultados precisos e reprodutíveis.
PURPOSE: This paper was aimed to study nerve regeneration after allografting using conventional point counting technique. INTRODUCTION:The interest towards nerve allografting has been growing since the recent development of better immunosuppressant drugs. METHODS: Three groups were studied: Group A - Lewis rats receiving nerve grafts from isogenic donors; Group B - Lewis rats receiving nerve grafts from Brown-Norway donor rats and treated with saline solution; Group C - Lewis rats receiving nerve grafts from Brown-Norway donor rats and treated with cyclosporine. Nerve regeneration was evaluated by histological analysis and by histomorphometric studies after 6 and 12 weeks. RESULTS: At 6 weeks, nerve fiber density and the percentage of neural tissue in the immunosupressed allograft group (C) were significantly higher than in group B. Allograft groups (B and C) showed significantly lower nerve fibers density and percentage of neural tissue when compared to the autograft group A at 6 or 12 weeks. CONCLUSIONS: We conclude that the point counting method was simpler to use than the computerized model, and yielded accurate and reproducible results.
Assuntos
Animais , Ratos , Animais de Laboratório/imunologia , Tolerância Imunológica , Regeneração Nervosa , Nervo Isquiático/fisiologia , Ciclosporina/uso terapêutico , Técnicas Histológicas , Terapia de Imunossupressão , Transplante HomólogoRESUMO
The present study observed the resistance to reinfection with Clonorchis sinensis in various experimental animals including mice, guinea pigs, rabbits, and dogs, as well as rats and hamsters. The resistance rates to reinfection in rats, mice, hamsters, guinea pigs, rabbits, and dogs were 79.7%, 58.0%, -12.6%, 54.8%, 62.6%, and 6.0%, respectively. Worms recovered from reinfected rats and mice were immature, and significantly smaller than those from the primarily infected (P < 0.01), whereas those from other animals were fully matured to adults. These findings indicate that the protective response against reinfection with C. sinensis is prominent in rats and mice, and that they may be a good animal model to investigate the mechanism of resistance to reinfection with C. sinensis.
Assuntos
Ratos , Coelhos , Camundongos , Cães , Cricetinae , Animais , Praziquantel/uso terapêutico , Cobaias , Clonorchis sinensis/imunologia , Clonorquíase/tratamento farmacológico , Anti-Helmínticos/uso terapêutico , Animais de Laboratório/imunologiaRESUMO
The local induction of humoral immune response to Entamoeba histolytica trophozoites in the gut has not been studied. This work reports some of our recent studied. This work reports some of our recent studies aimed to induce optimal immune responses against E. histolytica in mice and to describe a novel approach for monitoring mucosal immune responses induced in the gastrointestinal tract and expressed locally and systemically. We have compared the kinetics of both mucosal and systemic primary antibody responses to E. histolytica in the Peyer's patches (PP) and the spleen in Balb/c mice after a single dose of glutaraldehyde fixed amebas (GFA) by intragastric (IG), rectal (R), and intraperitoneal (IP) routes. The number of antibody-secreting cells directed to E. histolytica was assessed by the technique of ELISPOT on mitrocellulose filters. The antibody response to E. histolytica was detected in both PP and spleen with the three routes, indicating that either mucosal or systemic stimulation by GFA generates both types of response. We also determined the total antibody response in entestinal fluids and the antibody secretions from spleen and PP cells in vitro and found differences between male and female mice
Assuntos
Animais , Camundongos , Animais de Laboratório/imunologia , Antígenos , Relação Dose-Resposta Imunológica , Entamoeba histolytica/patogenicidade , Ensaio de Imunoadsorção Enzimática , Testes SorológicosRESUMO
Revisión de la inmunoterapia de la leishmaniasis, los modelos en los animales de laboratorio, y los progresos recientes en la vacunación experimental. En los últimos años se han producido progresos importantes que han contribuido sustancialmente a clasificar el papel de las interleucinas y otros mediadores químicos en la respuesta inmunitaria. Todos estos hallazgos abren la puerta para la producción de vacunas mejores y más inmunogénicas que en un futuro cercano habrán de utilizarse ventajosam,ente en los humanos