RESUMO
BACKGROUND: The Lewis histo-blood group system consists of 2 major antigens-Lea and Leb-and a sialyl Lewis antigen-carbohydrate antigen (CA) 19-9. We investigated the distribution of Lewis genotypes and evaluated the relationship between the Lewis/Secretor genotypes and the serum level of CA 19-9 in a Korean population to identify whether the serum CA 19-9 levels are influenced by the Lewis/Secretor genotypes. METHODS: The study included 242 individuals who had no malignancies. Lewis genotyping was performed for the 59T>G, 508G>A and 1067T>A polymorphic sites. The Secretor genotype was determined through analysis of the 357C>T and 385A>T polymorphic sites and the fusion gene. Serum CA 19-9 level was analyzed using an electrochemiluminescence immunoassay. RESULTS: Individuals carrying the 3 common genotypes-Le/Le, Le/le(59,508), and Le/le(59,1067)-accounted for 95% of the study population. In the Korean population, the allelic frequencies of Le, Le(59), le(59,508), and le(59,1067) were 0.731, 0.010, 0.223, and 0.035, respectively. We found a significant difference in serum CA 19-9 concentrations among the 9 Lewis/Secretor genotype groups (P<0.001). The serum CA 19-9 levels in subjects with genotype groups 1 and 2 (Le/- and se/se) were higher than those with genotype groups 3-6 (Le/- and Se/-; 15.63 vs 6.64 kU/L, P<0.001). CONCLUSIONS: Le/Le, Le/le(59,508), and Le/le(59,1067) are frequent Lewis genotypes in Koreans. Because serum CA 19-9 levels are significantly influenced by the Lewis/Secretor genotypes, caution is suggested when interpreting the serum CA 19-9 levels.
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Alelos , Povo Asiático/genética , Antígeno CA-19-9/sangue , Medições Luminescentes/métodos , Frequência do Gene , Genótipo , Imunoensaio/métodos , Antígenos do Grupo Sanguíneo de Lewis/genética , Fenótipo , Polimorfismo Genético , República da CoreiaRESUMO
Phenotype frequencies of the ABH and systems were obtained for 110 individuals belonging to the Arara and Wai-Wai (Katuena tribes) of northern Brazil, who speak languages of the Cariban Stock. only ABH secretor phenotypes were observed, and all of them were of the "O" blood group. The Lewis-positive phenotype was found among the Arara and is also the most frequent among the Wai-Wai indians. Le(a-b-) subjects occurred only among the Wai-Wai, suggesting more variation within this group than among the Arara.
Assuntos
Humanos , Indígenas Sul-Americanos , Sistema ABO de Grupos Sanguíneos/genética , Antígenos do Grupo Sanguíneo de Lewis/genética , Brasil , Fenótipo , Prevalência , Saliva/imunologia , Sistema ABO de Grupos Sanguíneos/análise , Antígenos do Grupo Sanguíneo de Lewis/análiseRESUMO
Descreve-se a distribuiçäo dos fenótipos e freqüências gênicas dos sistemas Lewis e Secretor em Caucasóides e Negróides do Rio de Janeiro. Observa-se em ambos grupos de cor o padräo de elevada prevalência do tipo Le(a-b) geralmente presente em Negros. Ambas amostras ajustam-se ao equilíbrio estimado para dois locos independentes e näo há diferença significativa nas suas freqüências gênicas
Assuntos
Humanos , Frequência do Gene , Fenótipo , Antígenos do Grupo Sanguíneo de Lewis/genética , População Negra , Brasil , População BrancaRESUMO
Quarenta e sete porcento das salivas de uma amostra aleatória de pessoas de grupo O Le(a-b-) possuem títulos apreciáveis de antígenos Lewis indicando que elas realmente pertencem ao genótipo Le/-. As proporçöes de secretores e näo-secretores de substância H entre Caucasóides e Negróides e fenótipos salivares Lewis säo similares. Estudo de uma família sugere que, seja pela causa de um gene atípico Le* ou por um gene repressor dominante, existe um efeito de bloqueio sobre a fixaçäo dos glicoesfingolipídeos Lewis na membrana da hemácia ou sobre a síntese ou circulaçäo dessas substâncias, porém, sem interferância na biossíntese das substâncias Lewis salivares. Discute-se a condiçäo imunogenética das pessoas Le(a-b-) como potencias elaboradoras de aloanticorpos Lewis em relaçäo com o transplante renal ou a transfusäo sangüínea incompatíveis
Assuntos
Humanos , Doadores de Sangue , Antígenos do Grupo Sanguíneo de Lewis/genética , Antígenos/análise , Brasil , Fenótipo , Saliva/imunologiaRESUMO
Blood and saliva from unselected blood donors at the Blood Bank, Siriraj Hospital were studied. Two Kell positive, two Rh negative and one Gerbiech negative were found, which could be considered as rare blood type in Thailand. The commonest Rh gene complex was CDe (R11 and the presence of CDE (Rz) in this study are the usual pattern of people in Southeast Asia. Fya is very common as in other people of Asia. In the Lewis system, the incidence of Le (a + b -) was 28.48% which agree well with our previous report 30.9%. There were 410 out of 1,668, (23.17%) who were found to be Lea non-secretor and 95 of them have Lewis antibodies in their sera. Aberrant secretion patterns were also found in this study, 5 people were found to secrete A or B substances according to their blood groups but no H substance was detectable. Further investigation of Lewis groups and secretion in Thailand are needed.