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1.
Artigo em Chinês | WPRIM | ID: wpr-254581

RESUMO

<p><b>AIM</b>To investigate the effect of endogenous endothelin-1 (ET-1) on cardiomyocyte apoptosis induced by hypoxia and its possible mechanism.</p><p><b>METHODS</b>Cultured neonatal rat cardiomyocytes were divided into control group and ET receptor antagonist group. Control group was given DMEM only and ET receptor antagonist group was treated with ET receptor subtype A (ET(A)) receptor antagonist BQ610 and BQ123 or ET(B) receptor antagonist BQ788 and subjected to hypoxia for 24 h. The presence of apoptosis in cardiomyocytes was evaluated by TUNEL analysis and flow cytometry (FCM).</p><p><b>RESULTS</b>TUNEL analysis showed that the percentage of positive apoptotic cells in BQ610 5 micromol/L group was 13.2% +/- 3.7%, significantly lower than that in hypoxia group (24.2% +/- 2.2%, P < 0.01). FCM showed that BQ123 (0.04, 0.2 and 1.0 micromol/L) inhibited hypoxia-induced cardiomyocyte apoptosis and increased cardiomyocyte survival rate in a dose-dependent manner, while BQ788 did not show such effects.</p><p><b>CONCLUSION</b>These findings suggest that endogenous ET-1 aggravates hypoxia-induced cardiomyocyte apoptosis and this effect is mediated through ET(A) receptor-dependent pathways.</p>


Assuntos
Animais , Ratos , Animais Recém-Nascidos , Apoptose , Hipóxia Celular , Células Cultivadas , Antagonistas do Receptor de Endotelina A , Antagonistas do Receptor de Endotelina B , Endotelina-1 , Fisiologia , Miócitos Cardíacos , Metabolismo , Patologia , Ratos Sprague-Dawley
2.
Zhonghua Wai Ke Za Zhi ; (12): 870-873, 2004.
Artigo em Chinês | WPRIM | ID: wpr-360942

RESUMO

<p><b>OBJECTIVE</b>To study the expression of ET receptor and the apoptosis after intervened with ET receptor antagonist in androgen-independent prostate cancer.</p><p><b>METHODS</b>PC3, an androgen-independent prostate cancer cell line, was used. The expression of ETA and ETB receptor in PC3 was measured through RT-PCR. After intervened with selective ETA and ETB receptor antagonist, the apoptosis in PC3 was studied through flow cytometry and electron microscope.</p><p><b>RESULTS</b>Clear signal was obtained in PC3 for ETA receptor mRNA transcript, while the signal for ETB receptor mRNA transcript was very weak. The expression of ETA receptor mRNA was obviously reduced and the apoptosis of PC3 cell was observed after intervened with selective ETA receptor antagonist. There was no change after intervened with selective ETB receptor antagonist.</p><p><b>CONCLUSION</b>ET-1 exerts its effects through the ETA receptor subtype and ETB receptor is silenced in PC3. The expression of ETA was reduced and the apoptosis was observed in PC3 when ETA receptor was blocked. It was dose-dependent.</p>


Assuntos
Humanos , Masculino , Androgênios , Fisiologia , Apoptose , Fisiologia , Antagonistas do Receptor de Endotelina A , Antagonistas do Receptor de Endotelina B , Endotelina-1 , Fisiologia , Técnicas In Vitro , Neoplasias Hormônio-Dependentes , Patologia , Oligopeptídeos , Peptídeos Cíclicos , Piperidinas , Neoplasias da Próstata , Patologia , Receptor de Endotelina A , Metabolismo , Fisiologia , Receptor de Endotelina B , Metabolismo , Fisiologia
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