Hepatitis B core antibody in blood donor in sistan-balutuestan province of Iran.

Background: Safety of blood donor is an important issue for a recipient of blood so all blood donors are to screened for viral such as HBV, HCV, and HIV. Nevertheless, infections sometime occur by blood and its products. Objective: Because we haven't got any awareness about isolated Hbc Ab from a blood donor who presented of occults B hepatitis which can transmit infection to blood recipient, we decided to evaluate HbcAb in a blood donor in this province. Materials and Methods: This is a cross-sectional study on a blood donor in Sistan-Balutuestan province in southeast of Iran in 2010. All individuals referred for blood donation recruited to this. After consent demographic data was recorded from each case, 5 ml blood sample was drawn and centrifuged to separate out the serum and then HbsAb, HbcAb was assayed. Data analyzed by SPSS 16 and frequency, chi- square test, and Fisher exact test was used and if P > 0.05 it was accented as significant association. Result: All individuals were men with age 55 10.5 years. The number of people who had free job was 149 (34.6%) and the number of people whose education level was diploma was 159 (36.9%). About 423 (98.1%) lived in urban areas. The mean weight of men was 76.6 13.7; about 259 (60.1%) men were married. A total of 22 (5.1%) had a positive smoking history. HBc Ab was positive in 87 (20.2%). Nearly all people had HBsAb titer more than 10 IU/L. Conclusion:This study showed that some of the blood donors had isolated HbcAb positive therefore we recommend HbcAb screening for blood donation.

study of serological markers of hepatitis B and C viruses in Istanbul, Turkey

This study was undertaken to investigate the prevalence of HBsAg, anti-HBs and anti-HCV positivity in Istanbul, Turkey. Subjects and The frequencies of HBsAg, anti-HBs and anti-HCV positivity were determined in 1,157 randomly selected patients attending the outpatient clinic of Istanbul University Hospital, Istanbul, Turkey, during the years 1998 and 2001. All patients underwent complete physical and various routine laboratory examinations. Of the 1,157 patients, the prevalence of HBsAg, anti-HBs and anti-HCV was 6.6, 28.1 and 2.4%, respectively. It appeared that having dental and surgical procedures formed the risk factors for HBV infection. HBsAg positivity in the health care workers was not different from that of the other professions, but anti-HBs was significantly higher in this group. Conclusions: Our findings indicate that HBV infection occurs more frequently than HCV in Istanbul, and this poses an important health problem in the community

Prevalencia de HCV y HBV en pacientes pediatricos hemodializados: estudio multicentrico / HCV and HBV prevalence in hemodialyzed pediatric patients: multicenter study

Los pacientes Hd constituyen una población de riesgo para la infección por los virus de las hepatitis B y C. El objetivo de este trabajo fue evaluar la prevalencia de la infección por HCV y HBV en un corte de una población de niños hemodializados. Se estudiaron 61 pacientes pediátricos en hemodiálisis, 12 de los cuales eran Tx, con edades comprendidas entre 2 y 20 años (media: 12,9), 23 varones y 38 mujeres. Se determinaron los anticuerps anti-HCV IgG por enzimoinmunoensayo (EIE) (Abbott 2 generación) y como método confirmatorio se utilizó inmunoblotting de péptidos sintéticos linearizados (LIA-TEK) (Organon). La detección de los marcadores para HBV fue por EIE y el nivel de actividad de las transaminasas se determinó por método cintético. El 19,7 por ciento de los niños estudiados fueron HCV positivos y el 29,5 por ciento fueron HBV positivos, de los cuales el 38,9 por ciento fueron HBsAg positivos el 50 por ciento Anti-HBs positivos. La infección por HCV y HBV se vió incrementada en relación al número de transfusiones y al tiempo de inclusión en Hd. La elevación de nivel de actividad de las transaminasas no fue un buen índice de infección para HBV y HCV en estos pacientes.

Infección con los virus de la hepatitis B y C en niños con trastornos congénitos de la coagulación / Infection with B and C hepatitis virus in children with congenital coagulation disorders

The prevalence of hepatitis B and C virus infections, transmitted by blood transfusions, was studied in 79 children with congenital coagulation disorders. Twenty nine percent had evidences of hepatitis B virus infection and 52 per cent evidences of hepatitis C virus infection. Older children and those with the higher number of transfussions had the highest rates of infections. It is concluded that children with congenital coagulation disorders constitute a high risk group for hepatitis B and C virus infections

Prevalencia de los marcadores del virus de la hepatitis B en personal de alto riesgo de un hospital general / Viral B hepatitis markers: it`s prevalence among hig-risk personal in a general hospital

Se estudia a todo el personal de los departamentos y servicios de un hospital general considerados de alto riesgo para la contaminación con el virus de la hepatitis B (Laboratorio Clínico, Banco de Sangre, Clínica Estomatológica, Anatomía Patológica, Gastroenterología, Nefrología, Terapia Intensiva y Unidad Quirúrgica), realizándose determinaciones del antígeno de superficie de la hepatitis B y su anticuerpo (HBsAg y anti HBs) y una encuesta epidemiológica. La prevalencia de estos marcadores en el personal estudiado fue del 21,1 %. El Servicio de Nefrología fue el de mayor prevalencia con 50 %. Los técnicos de la salud y enfermeras fueron las categorías ocupacionales con mayor prevalencia. El tiempo de trabajo dentro del área de alto riesgo, el contacto frecuente con la sangre y con el paciente resultaron ser factores de importancia en la prevalencia de los marcadores virales

Prevalencia de marcadores sericos del virus de la hepatitis B en el personal femenino de un hospital general / Prevalence of serum markers of hepatitis B virus in female personnel of a general hospital

Hemos determinado los marcadores del virus de la hepatitis B a 209 mujeres del personal de un hospital general: 12 Médicos, 90 ATS, 70 Auxiliares de clínica y de laboratorio y 37 personas de Personal de limpieza y office. La prevalencia fue del 15,31% (32 personas). Cuatro personas (1,91%) presentaron HBs-Ag. La prevalencia fue más baja que las encontradas por otros autores de nuestro país. Las prevalencias más altas se encontraron en el personal de limpieza y office, seguido de los ATS. Por Servicios, las prevalências más altas se encontraron en el personal en contacto con sangre y hemoderivados. No se encontraron marcadores en el personal de la UVI. La prevalencia aumentó y generalmente con la edad y la antigüedad. Asociamos los bajos índices de prevalencia encontrados por nosotros con los elevados porcentajes de personal joven (33,49%) tienen menos de 30 años y con poca antigüedad en el hospital (26,31% con menos de 4 años

Hepatitis B virus (HBV) infections in turtles

Thirty turtles (15 Clemys mutica and 15 Geoclemys reevesii) which were inoculated with human sera those were positive for hepatitis B surface antigen (HBsAg) and hepatitis B "e" antigen (HBeAg) were found to be infected with hepatitis B virus (HBV). The levels of HBV infection markers, such as HBsAg and antibody to HBsAg (anti-HBsAg), were retinely monitored in the turtles' serum for 46 weeks. Within two weeks of the inoculation, 42% of the turtles tested were positive for HBsAg, and their reciprocal titers as measured by reverse passive hemagglutination (RPHA) and enzyme linked immunoabsorbance assay (ELISA) ranged from 16 to 96. Within 20 weeks, the remaining turtles tested HBsAg positive, as confirmed by ELISA. At 20 weeks, all but one of the turtles exhibited changes in HBV blood marker from HBsAg to anti-HBs; the one exception was positive for both HBsAg and anti-HBs. At the 47th week, 7 animals were killed and their organs were examined for HBV infected cells utilizing an immunofluorescent technique. Numerous fluorescent cells which reacted with human anti-HBs nad anti-HBc were observed in the following organs: pancreas, liver, kidney, and brain. Histopathologically, edematous changes in hepatocytes and minor cellular infiltration attributed to an inflammatory response were noted. Liver and kidney cells from the infected animals were cultured, and HBV antigen positive cells for HBsAg and HBcAg were detected in the cultures. Throughout the experiment, HBsAg was detected in the supernatant by ELISA. Virus particles which were indistinguishable from Dane particles were seen in the cytoplasmic vacuoles of the cultured cells by electron microscopy. Finally, the presence of HBV DNA was established by molecular hybridization techniques in the culture supernatants of kidney cells from the infected turtles.

Dependencia de anticuerpos contra el Virus B de la hepatitis (AC-Ags VBH) en Caldas estudio en personal de salud / Prevalence of hepatitis B Virus antibodies in Caldas: study in health personnel

En el presente estudio realizado en el Departamento de Caldas, se analiza por primera vez la prevalencia de anticuerpos al antigeno de superficie del virus B de la Hepatitis (Ac-Ags VBh) mediante la tecnica de analisis inmunoenzimatico. Se procesaron 2226 sueros procedentes de personal vinculado al area de la salud y se incluyeron en dicho estudio 70 sueros controles provenientes de donadores de sangre del Hospital Universitario de Caldas. De los 2152 sueros del personal de salud, 81 fueron positivos (3.8 X 100), del grupo control, 4 fueron positivos (5.4 X 100). Las bacteriologas presentaron una positividad de 6.6 X 100 y los odontologos estudiados no tenian anticuerpos. En el estudio, se analizan los factores de sexo, edad, profesion, tiempo de vinculacion al area de trabajo, antecedentes clinicos, personales y familiares de Hepatitis e importancia de la prueba empleada