Effect of thyroxine on plasminogen activator and inhibitor activity in rat.

Thyroid hormones influence mineral metabolism, distribution of water and electrolytes and are therefore of great importance in the maintenance of homeostasis under normal and diseased conditions such as renal failure. The present study was carried out to determine the effect of thyroxine on fibrinolytic parameters such as plasminogen activators (PA) in rat kidney, levels of PA and plasminogen activator inhibitor (PAI), glucose in plasma and serum lipid profile injected with thyroxine (75 microg eltroxine/ 100 g(-1) body weight, ip for 7 days). Treatment increased PA activity significantly in rat kidneys. No changes were seen in PA, PAI and glucose in plasma of rats. There was significant decrease in total cholesterol and LDL-cholesterol levels in serum of treated group resulting in the decrease of HDL/LDL and total cholesterol/cholesterol ratios. However, triglycerides and VLDL showed significant higher activity in the serum of treated group as compared to controls. The results suggest beneficial effects of thyroxine treatment by increasing PA activity in kidney and reducing the cholesterol content in blood. It may be helpful to prevent hypercoagulable state by maintaining the normal homeostatic balance and restoring renal function.

Plasminogen activators and matrix metalloproteinase activities in experimental bladder carcinogenesis

To evaluate whether or not the organophosphorus dimethoate insecticide play a vital role in the high incidence of damage of bladder organ in experimental animals. [2] To investigate the possible promoting or synergistic effects of organophosphorus compounds on the potent carcinogenic nitrosamines. [3] To protect the bladder organ from the damaging effect of either organophosphorus or nitrosaniines. Quantitative zymography was used to estimate plasminogen activators [PAs] and matrix metalloproteinases [MMPs] in male wistar albino rats urinary bladder after treatment with dimethoate in the presence and absence of diethyl nitrosoamine[DEN]. High level expressions of PAs and MMPs have been correlated with urinary bladder hyperplasia and tumors. PAs activity was significantly [p<0.05] increased in DEN initiation and dimethoate promotion in urinary bladder. MMPs expressed significant elevation [p<0.01] after the initiation with DEN and the treatment with dimethoate. Caproic acid has no significant effect throughout the experiments. Dimethoate is not carcinogenic hut it acts as promoter. PAs and MMPs may he important biochemical factors affecting urinary bladder carcinogenesis

Fibrinolytic activity in obliterative arterial diseases

This study was designed with the aim of assessing the fibrinolytic status of patients suffering from obliterative arterial disease [OAD] as compared to healthy controls. Twenty-five patients with arterial obliterative diseases presented acutely were chosen for fibrinolytic assay and compared with 25 healthy controls. Twelve patients had critical ischemia and 15 patients were diabetics. Twenty-one patients had lower limb ischemia while, 4 patients had upper limb ischemia. The results of the study suggested that the fibrinolytic system is significantly impaired in cases of acute or severely ischemic patients when compared to controls. It was concluded that fibrinolytic assay could be added as a routine investigation in cases of critical ischemia and early onset atherosclerosis. Therapeutic options could be adjusted according to the fibrinolytic assay results