Doenca de Jorge Lobo e sua relacao com os antigenos do sistema HLA / Disease Jorge Lobo and his relationship with the antigens of the HLA system

A doenca de Jorge Lobo e uma micose causada pelo fungo Lacazia loboi (L.loboi), o qual se assemelha filogenetica e antigeneticamente ao Paracoccidioides brasiliensis (P.brasiliensis). Devido as caracteristicas epidemiologicas e aos poucos estudos relacionados aos apectos imunologicos dessa doenca, o objetivo desse trabalho foi pesquisar a frequencia dos antigenos HLA de classe I e classe II em 21 pacientes portadores da doenca de Jorge Lobo e comparar com populacao controle. As tipagens HLA de classe I foram realizadas pela tecnica de microlinfocitotoxicidade e as de classe II pelo metodo de PCR-SSP. Como controles, utilizaram-se duas populacoes: uma do Estado do Acre e outra da populacao brasileira, segundo os dados publicados no 11th IHW no Japao

Inoculation of Lacazia loboi into the subcutaneous tissue of the hamster cheek pouch

The subcutaneous tissue of the hamster cheek pouch, a site of immunologic privilege, has been used to investigate the potential infectivity of different types of parasites. It has been demonstrated that the implantation of fragments of lesions induced by the fungus Lacazia loboi, the etiologic agent of Jorge Lobo's disease, into the subcutaneous tissue of the hamster cheek pouch resulted in parasite multiplication and dissemination to satellite lymph nodes16. Here we describe the evolution of lesions induced by the inoculation of the isolated fungus into this immunologically privileged site. The morphology of the inflammatory response and fungal viability and proliferation were evaluated. Inoculation of the fungus into the cheek pouch induced histiocytic granulomas with rare lymphocytes. Although fungal cells were detected for a period of up to 180 days in these lesions, the fungi lost viability after the first day of inoculation. In contrast, when the parasite was inoculated into the footpad, non-organized histiocytic lesions were observed. Langhan's giant cells, lymphocytes and fungal particles were observed in these lesions. Fungal viability was observed up to 60 days after inoculation and non-viable parasites were present in the persistent lesions up to 180 days post-inoculation. These data indicate that the subcutaneous tissue of the hamster cheek pouch is not a suitable site for the proliferation of Lacazia loboi when the fungus isolated from human tissues is tested.

Avaliação imunológica de pacientes portadores de doença de Jorge Lobo / Immunological Evaluation of patients with Jorge Lobo's disease

Os autores investigaram a resposta imune humoral e celular de 16 pacientes portadores de doença de Jorge Lobo, através das seguintes avaliações: pesquisa de crioglobulinas, proteinas C reativa, auto-anticorpos, quantificação das imunoglobulinas séricas e do componente C3 do complemento e realização de etstes cutâneos de hipersensibilidades tardia com antígenos inespecíficos. Os resultados revelaram níveis séricos elevados de imunoglobulinas, principalmente IgC, bem como elevação dos níveis de C3. A maioria dos pacientes não reagiu aos testes intradérmicos realizados. Em conjunto os resultados sugerem alteração da resposta imune humoral e depressão parcial da resposta imune celular.