Synthesis and antiproliferative activities of novel 5'-Schiff-base group substituted psoralen derivatives / 药学学报

It was found that psoralen derivative could perform a Friedel-Crafts acylation smoothly with acetic anhydride to give 5'-acetylpsoralen in a 73% yield. In the presence of boron trifluoride etherate, 5'-acetylpsoralen reacted with both aromatic amines and aliphatic amine smoothly to afford 5'-Schiff-base group substituted psoralen derivatives in 72%-92% yields. The novel synthetic method has the advantages of cheap materials, mild reaction conditions, good yields and high regioselectivity in the Friedel-Crafts acylation. Cell viability assay by MTT demonstrates that some of the psoralen derivatives 6 have antiproliferative activities.

Characterization of chemical components of essential oil from flowers of Chrysanthemum morifolium produced in Anhui province / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the chemical components of the essential oil extracted from six cultivars' Juhua (Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju) produced in Anhui province of China, which were breed from Chrysanthemum morifolium, and to provide scientific basis for quality control.</p><p><b>METHOD</b>The essential oils of the six cultivars'Juhua were extracted by water-steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS.</p><p><b>RESULT</b>From Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju, 247, 226, 246, 182, 216 and 122 chromatographic peaks were detected, among them 75, 54, 78, 50, 53 and 43 components were identified,which were composed of 85.67%, 82.80%, 81.38%, 73.22%, 71.51% and 72.87% of the total essential oil, respectively.</p><p><b>CONCLUSION</b>Monoterpenoid compounds were higher more than sesquiterpenoid compounds in the five juhua except for Chuju. There was no difference in the constituents of essential oil of Zaogongju and Wangongju. And verbenyl acetate was the main chemical constituent and composed of 32.10% and 37.85% of the total essential oil, respectively. (1R)-Camphor and bisabolol oxide A were the main chemical constituents in Huangyaoju, which were composed of 28.70% and 12.58% of the total essential oil, respectively. beta-Selinene and borneol were the main chemical constituents in Chuju, which were composed of 17.85% and 12.84% of the total essential oil, respectively. Eucalyptol was the main chemical constituent in Xiaoboju, which were composed of 21.33%. Verbene oxides and chrysanthenone were composed of 25.32% and 8.26% of the total essential oil, respectively, in the Daboju. The common chemical constituents were camphene, borneol, bornyl aceate, (1R)-camphor, (-)-4-terpineol, alpha-terpineol, eucalyptol, cis-caryophyllene, caryophyllene oxide, juniper camphor, beta-sesquiphellandrene, alpha-curcumene and beta-farnesene in the six cultivars' Juhua produced in Anhui province of China. The resultant data provided the further evidences to distinguish Juhua' cultivars and scientific basis for quality control of Juhua.</p>

Histopathological changes of testes and eyes by neutron irradiation with boron compounds in mice

This study was performed to investigate the biological effects of boron neutron capture therapy (BNCT) on the testes and eyes in mice using HANARO Nuclear Reactor, Korea Atomic Energy Research Institute. BNCT relies on the high capacity of (10)B in capturing thermal neutrons. Sodium borocaptate (BSH, 75 ppm, iv) and boronophenylalanine (BPA, 750 ppm, ip) have been used as the boron delivery agents. Mice were irradiated with neutron (flux: 1.036739E +09, Fluence 9.600200E+12) by lying flat pose for 30 (10 Gy) or 100 min (33 Gy) with or without boron carrier treatment. In 45 days of irradiation, histopathological changes of the testes and eyes were examined. Thirty-three Gy neutron irradiation for 100 min induced testicular atrophy in which some of seminiferous tubules showed complete depletion of spermatogenic germ cells. Lens epithelial cells and lens fiber were swollen and showed granular changes in an exposure time dependent manner. However, boron carrier treatment had no significant effect on the lesions. These results suggest that the examination of histopathological changes of lens and testis can be used as "biological dosimeters" for gauging radiation responses and the HANARO Nuclear Reactor has sufficient capacities for the BNCT.

Avaliaçäo do teor de arsênio total em pescado de água doce / Evaluation of arsenic content in fish from freshwater

Foi determinado o teor de arsênio total em polpas e filés das seguintes espécies de peixes de água doce: Pacu (piaractus mesopotamicus), Matrinxä (Brycon lundi), Carpa (Cyprinius carpio), Tilápia (Oreochromus niloticus), Curimbatá (Prochilodus sp), Tambaqui (Colossoma maceropomum), Bagre Africano (Clarias sp), procedentes do centro de Pesquisa e Treinamento em Aquicultura, Pirassununga - Säo Paulo. A determinaçäo do elemento arsênio foi realizada por espectrometria de emissäo atômica com fonte de plasma de argônio induzido (ICP-AES) acoplado a um gerador de hidretos, nas amostras previamente mineralizadas por via seca com nitrato de magnésio. Os teores obtidos de arsênio (ug kg-1) para os filés e polpas foram, respectivamente: Pacu (49 e 8), Matrinxä (27 e 24), Carpa (66 e 209), Tilápia (37 e 277), Curimbatá (30 e 93), Tambaqui (42 e 24) e Bagre (43). Em todas as amostras foram encontrados níveis de arsênio inferiores ao recomendado pela legislaçäo brasileira. (AU)

The level of arsenic was determined in pulps and steak of the following freshwater Fish species: Pacu (Piaractus mesopotamicusi, Matrinxã (Brycon lundii, Carp (Cyprinius carpio], Tilápia tOreochromis niloticusi,Curimbatá tProchilodus sp),Tambaqui (Colossoma macropomumi, Bagre Africano (Clarias sp). The samples were obtained from the Center of Research and Training in Agriculture, Pirassununga, São Paulo.The determination of the arsenic elemento was erforrned By hydride generator-inductively coupled plasma atomic emission spectrometry (ICP-AES), after minera- lizing by drying with magnesium nitrate of the samples.The quantity obtained of arsenic in ug kg' from the fishes for steak and pulps respectively were: Pacu (49 e 8), Matrinxã (27 e 24), Carp (66 e 209), Tilápia (37 e 277), Curimbatá (30 e 93), Tambaqui (42 e 24) e Baqre (43). Arsenic levels below the ecommended Brazilian legislation were found in ali samples. (AU)