The effect of anti-hypertensive drugs on the obstructive pancreatitis in rats / Efeitos de fármacos anti-hipertensivos sobre pancreatite obstrutiva em ratos

PURPOSE: To investigate the effect of ACE inhibitor, lisinopril and AT1 blocker, losartan, on the obstructive pancreatitis in rat. METHODS: Acute pancreatitis in rats (n=21) was induced for a common hepatic duct were ligated proximal to its entry into the pancreas and the common bile - pancreatic duct were also ligated near its junction with the duodenum, under ether anesthesia, after which the abdomen were closed. The animals was divided in tree groups, being two treated and control group. The animals was treated with Losartan and Lisinopril at the dose of 10µg/Kg body weight per day, i.p., in a proportional volume, for five days, before and after treatement. RESULTS: The inflammation, collagen deposition in the pancreas of treated animals were smaller, suggesting that the use of antihypertensive agents interfered positively in the depletion of the injury of the pancreas. Scythe showed a correlation between activity of pancreatic stellate cells (PSCs) lower in treated animals when compared to control. CONCLUSION: The pancreatic stellate cells strength are involved in collagen production during acute pancreatitis and why antihypertensive drugs such as lisinopril and losartan may possibly have beneficial effects in reducing pancreatic fibrosis in models of experimental obstructive pancreatitis.

OBJETIVO: Investigar o efeito de um inibidor da ECA, lisinopril e bloqueador AT1, losartan, a pancreatite obstrutiva em ratos. MÉTODOS: Pancreatite aguda em ratos (n = 21) foi induzida por um ducto hepático comum foram ligados proximal à sua entrada no pâncreas e da bílis comum - ducto pancreático também foram ligados perto de sua junção com o duodeno, sob anestesia com éter, após o que abdome foram fechadas. Os animais foram divididos em três grupos, sendo dois tratados eo grupo controle. Os animais foram tratados com lisinopril e losartan na dose de 10µg/Kg de peso corporal por dia, IP, em um volume proporcional, por cinco dias, antes e depois do tratamento com. RESULTADOS: A inflamação, deposição de colágeno no pâncreas de animais tratados foram menores, sugerindo que o uso de agentes anti-hipertensivos interferiram positivamente na diminuição da lesão do pâncreas. Este estudo mostrou uma correlação entre a atividade das células pancreáticas estreladas (CSP) menor nos animais tratados quando comparados ao control. CONCLUSÃO: A força das células pancreáticas estreladas está envolvida na produção de colágeno durante a pancreatite aguda e por medicamentos anti-hipertensivos, tais como lisinopril e losartan pode eventualmente ter efeitos benéficos na redução da fibrose do pâncreas em modelos experimentais de pancreatite obstrutiva.

Pancreatic stellate cells and alcohol induced chronic pancreatitis in albino rats: histological and immunohistochemical study

Pancreatic stellate cells [PaSCs] are myofibroblast-like cells found in the areas of pancreas that have exocrine function. The aim of the present work was to study the pancreatic stellate cells in the exocrine pancreas and to explain their role in pancreatic fibrosis associated with chronic alcoholic pancreatitis. Forty adult male rats were used in this study. The animals were divided into two groups; control and experimental. The experimental group was given alcohol by intragastric tube daily for 8 weeks. At the end of the experiment the animals were sacrificed and the pancreatic tissue was taken, processed and semithin and paraffin sections were obtained. Paraffin sections were stained with H and E, Mallory trichrome and immunohistochemical stains for detection of alpha smooth muscle actin [alpha-SMA] in PaSCs. Eight weeks after alcohol administration, the pancreatic tissue was traversed by thick connective tissue septa. The interlobular ducts were thickened, distorted and dilated. Some pancreatic acini were distorted, dilated and showed vacuolation and degeneration of their acinar cells. PaSCs were detected in three main sites like in control pancreas; periacinar, periductal and perivascular, but they showed an apparent increase in their number, decrease in their lipid droplets and transformtion into myofibroblast like cells with marked expression of alpha-SMA especially in the fibrotic areas. From the present work it was concluded that the cells which were detected seem to be the pancreatic stellate cells which could play an important role in the development of pancreatic fibrosis associated with alcoholic chronic pancreatitis. Therefore, understanding the biology of PaSCs may offers potential therapeutic targets for the treatment and prevention of these diseases