Comparación de sensibilidad a los antimicrobianos por método de difusión y dilución en Agar frente a ciprofloxina y eritromicina en aislamiento de Campylobacter spp / Comparison of sensitivity to the antimicrobials by diffusion method and dilution in Agar against ciprofloxina and eritromicina in isolation of Campylobacter

Estudio prospectivo, comparativo, realizado en el Laboratorio Central de Salud Pública (LCSP). Se incluyó en el estudio 58 cepas de Campylobacter spp., aisladas de heces humanas y que fueron remitidas de diferentes centros sanitarios al LCSP. Estudia la sensibilidad de la Ciprofloxicina (IP) y Eritromicina (ERY) por 2 métodos: el método de difusión de Kirby- Bauer y siguiendo recomendaciones del Clinical and Laboratory Standards Institute y el método de dilución en agar (macrodilución), utilizando el multi-inoculador de Steer.Para el análisis de la correlación utilizamos el scatter plot y el porcentaje de concordancia

Occurrence of Campylobacter species in poultry forms in Lagos area of Nigeria.

Samples of poultry droppings were collected from fifty poultry farms in Lagos area of Nigeria. Campylobacter species were isolated from serially diluted samples using Bolton selective enrichment medium under microaerophilic condition at 42 degrees C. Samples of water, both treated and untreated used in the farms were also analysed in the same manner. Fifteen Campylobacter strains were isolated from the droppings. All the strains were identified using biochemical characteristics differentiating Campylobacter species. They were identified as hippurate positive (C. jejuni) and negative species (C. coli). They were biotype' using the extended scheme described by Lior (1984). Campylobacter coli biotype 'I was the most prevalent (66.66%) followed by C. jejuni biotype 1 (20.00%). The biotype ii of the two species accounted for 6.67% each. The C. jejuni biotype III and IV were not isolated during the study. The preponderance of C. coli indicates that poultry can be a major reservoir for the spread of human campylobacteriosis. Poultry farmers, therefore, must ensure prompt and adequate means of disposal of waste materials as well as maintaining good sanitary conditions.