Determination of cefotaxime, cefoperazone, ceftazidime and cefadroxil using surface plasmon resonance band of silver nanoparticles

The aim of this work is to evaluate simple, sensitive, effective and validated procedures for the determination of cefotaxime, cefoperazone, ceftazidime and cefadroxil. In this study, the methods based on the ability of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of Polyvinyl Pyrrolidone (PVP) as a stabilizing agent producing very intense surface plasmon resonance peak of Ag-NPs (λmax. = 410-430 nm). The plasmon absorbance of the Ag-NPs allows the quantitative spectrophotometric determination of the cited drugs. The calibration curves are linear with concentration ranges of 0.4-3.2, 1-8, 0.5-4.0 and 1.5-9.0 µg/mL for cefotaxime, cefoperazone, ceftazidime and cefadroxil, respectively. Apparent molar absorptivity, detection and quantitative limits are calculated. Applications of the proposed methods to representative pharmaceutical formulations are successfully presented. The extracellular synthesis of nanoparticles is fast, and the method doesn't require various elaborate treatments and tedious extraction procedures.

use of 2,6-dichloro quinone chloride for the colorimetric determination of some antibiotics

A simple, sensitive and facile colorimetric method for quantitative determination of amoxicillin, cefadroxil and ceforperazone is developed. The method is based on the formation of blue colored chromogen upon the reaction of the investigated drugs with 2,6-dichloroquinone chlorimide in alkaline medium. The optimal experimental parameters for the color production are selected. Obedience the Beer's law permitted the successful application of the developed method for the assay of investigated drugs in their pharmaceutical dosage forms. The results obtained are compared with the reported method