Effect of vitamin E supplementation on antioxidant enzymes and lipid peroxidation in myocardial infarction patients

Oxidative stress is increased in patients with myocardial infarction [MI] owing to increase in free radical production. Alteration in the levels of antioxidant enzymes in these patients are a consequence of either increased production of free radicals or depletion of endogenous antioxidants. It is believed that vitamin E supplementation reduces MI by scavenging free radicals and by ameliorating enzyme activity. However, the outcomes of clinical trials with vitamin E in MI prevention have been mixed. The present study was therefore, undertaken to assess the status of antioxidant markers: superoxide dismutases [SOD], ceruloplasmin, and lipid peroxidation i.e., malondialdehyde [MDA] levels in the blood samples of MI patients and to investigate the effect of vitamin E supplementation in ameliorating the levels of antioxidant enzymes and malondialdehyde in Ml subjects. 120 Ml patients 30 to 65 years of age were taken for the study and 72 healthy individuals served as controls. In vitro vitamin E supplementation study in the blood samples of the respective MI subjects was performed. Vitamin E supplementation brought about an improved antioxidant status with significantly raised, SOD levels [22.4%, p<0.05] and simultaneously depleted MDA levels [32.58%, p<0.001]. Ceruloplasmin levels however, remained unaltered [p<0.1]. These findings further support the preventive and helpful role of vitamin E supplementation in reducing oxidative stress levels in MI patients

effect of ascorbic acid on serum level of copper, zinc, ceruloplasmin enzyme activity and iron parameters in men

Undue use of vitamin C has resulted concerns over some the harmful effects of it. To examine the effect of vitamin C on serum level of copper, zinc, iron parameters, and the ceruloplasmin enzyme activity. This was a double-blind clinical trial carried out in 1999 in Iran Research Institute for Nutrition and Food Sciences in healthy men. Volunteers were divided into 3 groups. Ascorbic acid was given to first two groups at the concentrations of 500mg/day and 1000 mg/day, respectively. No ascorbic acid was delivered to the third group [control group]. Fastting blood samples were collected in the beginning and at the end of the study period [6th week]. Copper and zinc concentrations were determined by atomic absorption; ceruloplasmin enzyme activity and vitamin C levels by colorimetric method; iron, TIBC and hemoglobin by employing a kit from zist-chimi company; and the percent of transferring saturation was calculated using the formula: serum iron/TIBCx100. Dietary consumption pattern in the beginning and at the end of the study period were recorded using 24hr dietary recall questionnaire for one day. Mean Blood ascorbic acid concentration increased by 67% [p=0.001], 76% [p=0.001], and 23% [p=0.04] in groups marked as 500 mg/day, 1000 mg/day ascorbic acid and control groups, respectively. This was statistically significant at the end of study. Mean ceruloplasmin enzyme activity decreased [p=0.004] in group 1000 mg/day ascorbic acid at the 6th week which was statistically significant. In spite of changes in serum copper and iron levels, these changes were not significant, statistically. Vitamin C had no effect on zinc concentration. No significant change was present in food consumption pattern during the study period. Based on present data, vitamin supplementation at daily doses of 500 and 1000 mg cannot be recommended and further are needed in this regard

A ceruloplasmina como enzima marcadora de atividade neoplásica maligna / The ceruloplasmin with enzyme activity maligna neoplasm

Os autores estudaram os níveis de atividade oxidásica da cerulopasmina sérica em ratos machos da linhagem isogênica U inoculados com um sarcoma singênico. Hiperceruloplasminemia estatisticamente significativa foi observada em todas as fases de crescimento tumoral. Valores normais de cerulopasmina foram obtidos unicamente após a 1ª dose de ciclofosfamida (100 mg/Kg, por via intraperitoneal). A subseqüente administraçäo semanal da droga näo bloqueou o reaparecimento da hiperceruloplasminemia nos animais portadores de sarcoma. Ratos normais tratados pela ciclofosfamida, de acordo com o mesmo esquema, apresentaram queda na atividade enzimática após a 1ª dose. Os autores concluem que a hiperceruloplasminemia sugere a presença latente de células neoplásicas viáveis e pode servir como um teste bioquímico para monitorizar o processo maligno