High seroprevalence of Simkania negevensis in Jordan

The bacterium Simkania negevensis is a germ associated with respiratory diseases. This study aims at estimating the prevalence of Simkania in the Jordanian population. Serum samples from 664 Jordanian males and females, aged 2 to 86 years were collected. IgG and IgM Simkania-specific antibodies were detected using an indirect immunofluorescence test. Seropositivity titers for IgG and IgM were defined as 1:8 and 1:10, respectively. The overall prevalence of IgG antibody in all examined Jordanian nationals was 58.4%. IgG seropositivity was low in children under the age of 10 years (34.2%), and increased rapidly with age and ranged between 49.4% and 72%. Simkania-specific IgM was detected in 24.8% of subjects. IgM prevalence in children under 10 years was lowest (10.5%) and increased in older ages and remained above 20%. Overall detection rates of both IgG and IgM were significantly higher in females than males (60.7% vs. 54.5% for IgG and 26.7% vs. 21.7% for IgM). These data indicate that Simkania infection is highly prevalent in Jordan. The high level of seropositivity is most likely maintained by re-infections or chronic infections. Our data may serve as a basis to elucidate the pathogenesis of Simkania in Jordan.

The discovery of naked cluster particles of Parachlamydia and its developmental mechanism / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the survival and developmental morphology of Parachlamydia (BN9) within Acanthamoeba.</p><p><b>METHODS</b>The morphology of BN9 within Acanthamoeba was studied by inverted phase contrast microscope, electron microscope, Gimenez and AO-staining with amoebal co-culture.</p><p><b>RESULTS</b>The endosomal maturation-blocked were formed after the egress of BN9. Two developmental stages-elementary and reticulate bodies, were both observed within the vacuoles. The reticulate bodies, multiplicated by binary fission, were located mainly within the vacuoles, while the elementary bodies can also be located in the plasma individually. The naked cluster particles were observed after the trophozoites cytolysis with Gimenez-staining. The light infectious trophozoites could encyst, and elementary bodies could survive within the mature cysts.</p><p><b>CONCLUSION</b>The egress of BN9 could form the endosomal maturation-blocked, which was presented in two developmental stages-elementary and reticulate bodies. It exhibited the cytolysin activity that could lyse the infectious trophozoites and were expelled in the vesicles. A few light infected amoeba could encyst with survival elementary bodies in the plasma.</p>

Prevalent of lower genital tract infection in women who visited some offices of Mother and Children Health Protection/ Family Planing Offices in Hµ Néi City

A randomized study involved 1,000 women who were 18-44 years of old at Hµ Néi Obstetric-Gynecological Hospital, Hai Bµ Tr­ng Maternity Clinic, and Obstetric Department of Thanh Nhµn Hospital. The cross-sectional survey showed that mean age of participants was 34.1 years. Mean age of leukorrhoea group was 33.4 years and of non-leukorrhoea group was 34.7 years. Mean age at time of marriage was 23.5. Most of participants were housewifery and official, and have finished only primary and secondary school. Severe leukorrhoea was seen in 50.4% of the participants. Most of them (98.4%) have sex relationship only with their husbands. 28.2% have not used any contraceptive method. 0.2% of the participants have external genital ulcer. 31.7% have cervical erosion. 16.5% have pelvicitis.

The genital chlamydial infection in pregnant woman in Ha noi

The prevalence of Chlamydial infection in pregnant women was 8,1%. Pregnant women under 25 years old were at high risk for Chlamydial infection. Chlamydial infection was not associated with gestational age. There is no difference about the prevalence of Chlamydial infection between the rural and urban women. Oral amoxicillin had a efficacy of 82,2% after 1 stage and 86,4% after 2 stages of treatment. The result suggested that amoxicillin is a safe, well tolerated and effective for treatment of Chlamydia in pregnant women.

Taxonomía e importancia clínica de las nuevas familias del orden chlamydiales / Taxonomy and clinical importance of the new families of the order chlamydiales

Esta revisión comprende los cambios trascendentales que se han producido recientemente en la taxonomía de clamidias, la importancia clínica de las nuevas especies del orden Chlamydiales y los métodos para su identificación. La nueva clasificación del orden Chlamydiales se basa en las características fenotípicas, antigénicas, rango de huésped y análisis filogenético del operón ribosomal y proporciona un procedimiento consistente para la identificación de las actuales especies y el establecimiento de nuevos taxa. Los Chlamydiales son patógenos importantes y los animales. Su capacidad de producir enfermedades respiratorias, entre otras formas de presentación, es una característica frecuente de estos microorganismos, estando presente en Chlamydophila pneumoniae, Chamydophila psittaci, Chlamydia trachomatis y en las familias recientemente descritas Simkaniaceace y Parachlamydiaceae. Los Chlamydiales son las únicas bacterias intracelulares exclusivas de células eucarióticas. El hallazgo de la familia Parachlamydiaceae, con los géneros Parachlamydia y Neochlamydia, ambos endosimbiontes de amebas de vida libre, señala la diversidad filogenética del orden y sugiere un reservorio ambiental de mayor importancia

Study on diminishing the microorganism infection on the drug Hepamarin by radiation with gamma Co-60

Study on decontamination of microorganisms on hepamarin, an herbal drug by gamma Co-60 radiation has been carried out with doses of 5 and 10kGy. the results showed that the contamination of microorganism on hepamarin drug decreased sharply from 108 (non-irradiated) to 105 and 4.102 microorganisms/g for sample irradiated at dose of 5 and 10 kGy, respectively. Post irradiation effect of decotamination of microorganisms on hepamarin was maintained during storage. The obtained data indicated that gamma Co-60 radiation is suitable for decontamination of microorganisms on hepamarin.