RESUMO
Attempts were made to examine the effect of paralytic shellfish poisoning toxins (PSP) on hepatic xenobiotic-metabolizing enzymes (XMEs) of tiger puffer (Takifugu rubripes). Two groups of nontoxic tiger fish were analyzed, and one group was fed with a PSP-containing diet (PSP group), and another with a PSP-free diet (control group). After 60 days of feeding, they were compared to each other mainly in terms of the activity of XMEs. Both groups did not differ from each other significantly in body weight gain, hepatosomatic index, and condition factor Hepatic level of cytochrome P450 was lower in PSP group than control group. NADPH-cytochrome P450 reductase, NADH-cytochrome b5 reductase, and ethoxyresorufin-O-deethylase (EROD) exhibited a reduced activity in PSP group than control group. Statistical analysis found that the activity or concentration of those enzymes correlated with the hepatic level of PSR with r2=0.497-0.611.
Assuntos
Animais , Citocromo P-450 CYP1A1/metabolismo , Citocromo-B(5) Redutase/metabolismo , Dieta/veterinária , Relação Dose-Resposta a Droga , Fígado/efeitos dos fármacos , Toxinas Marinhas/toxicidade , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Valores de Referência , Frutos do Mar/toxicidade , Takifugu/crescimento & desenvolvimento , Fatores de Tempo , Aumento de Peso/efeitos dos fármacos , Xenobióticos/metabolismoRESUMO
Os níveis de NADH-redutase de metemoglobina e de hemoglobina, bem como a taxa de reticulócitos, foram determinados em 60 hansenianos adultos (30 homens e 30 mulheres) sob sulfonoterapia. Os resultados encontrados permitiram concluir que tanto o nível de hemoglobina quanto a taxa de reticulócitos näo têm influência significativa na determinaçäo da atividade da NADH-redutase de metemoglobina, e que a atividade dessa enzima é mais alta nos pacientes do sexo feminino