Water-soluble non-alkaloid chemical constituents contained in Corydalis yanhusuo by trimethylsilyl derivatization GC-MS / 中国中药杂志

<p><b>OBJECTIVE</b>To study the water-soluble non-alkaloid chemical constituents of Corydalis yanhusuo.</p><p><b>METHOD</b>The 80% alcohol extracts of C. yanhusuo passed through DA201 macroporous resin. Eluted fractions were collected and passed though 732 # cation exchange resin. Water eluate was collected, dried and derived with trimethylsilane. Gas Chromatography/Mass Spectrometry and NIST 2005 library were adopted for MS/MS mass spectrogram to infer the compound structure.</p><p><b>RESULT</b>Sixteen compounds were tentatively identified from about fifty peaks detected by GC-MS and identified as hydroxyl and carboxyl polar compounds.</p><p><b>CONCLUSION</b>These sixteen compounds were found for the first time in C. yanhusuo. The results provide scientific basis for in-depth development of C. yanhusuo.</p>

Removal of styrene from air by natural and modified zeolite

Changing the surface characteristics of raw materials has created new prospects for catalysts and adsorption technology. Natural and modified zeolite with trimethylchlorosilane [TMSCI] has been used to adsorb styrene. Dynamic adsorption tests were performed with a number of standard characterization techniques and zeolite particles were examined in three sizes: less than 1 mm, 1-2 mm, and 2-4 mm. The gas concentration was 20 ppm, 40 ppm, and 60 ppm. Three flow rates were tested in this study: 0.5 l/min, 0.75 l/min, and 1 l/min. The sorption of Styrene depended on the particle diameter size, gas flow rate, and inlet gas concentration. The optimum size for adsorption was less than 1 mm, the optimum flow rate was 0.5 l/min, and the optimum gas concentration was 20 ppm. The adsorption capacity decreased with increasing size, flow rate, and concentration of the pollutant. The adsorption by the surface-modified zeolite increased by as much as 100% compared with natural zeolite. Therefore, the surface-modified natural zeolite may be utilized for many adsorption applications. This study shows the importance of chemical surface modification and confirms similar findings of other studies

Establishment of an animal model with thymic lymphoma in mice / 中国实验血液学杂志

The objective of study was to establish an animal model with thymic lymphoma in mice induced by intraperitoneal injection of DNA alkylating agent N-methyl-N-nitrosourea (MNU). Male and female mice of the C57BL/6 strain were injected by the intraperitoneal route with MNU solution in a dosage of 50 mg/kg body weight. The injection was repeated at week 8. Following injection of MNU, the general status of mice was observed. All mice were sacrificed for autopsy at the 22nd experimental week. Complete gross examination was performed for detection of tumor masses. The results showed that at the 22nd week, the incidence of thymic lymphoma in MNU-treated animals was 67.5% (27/40). No significant sex difference in the incidence of thymic lymphoma was observed. In conclusions, an animal model with thymic lymphoma in mice can be established by twice intraperitoneal administration of MNU. The biological behavior of the induced tumors resembles to those of human thymic lymphoma derived from thymic T-cells.

Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry

For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.

Enzymatic generation of electronically excited triplet species: photobiochemistry without light

The generation of electronically excited molecules in the singlet state as a result of chemical or biochemical reaction has been known for a very long time, as attested to by chemiluminescence and bioluminescence. Since the late fifties, we have been interested in the possibility of biochemical generation of electronically excited triplet species. Molecules in the triplet state have a much longer intrinsic lifetime than those in the corresponding excited singlet state, whereby the former are much more suitable to participate in bimolecular processes, such as reactivity and energy transfer. This is expected to be especially important in biochemical/biological systems, where possible reactants or enery acceptors may exist in low concentration. Therefore, triplet species might play useful and/or deleterious roles in these systems. Progress had to wait for the demonstration that triplet species can also be generated chemically. This came with the advent of dioxetane/dioxetanone chemistry in the late sixties/early seventies. The cleavage of these fourmembered cyclic peroxides can yield a carbonyl group in the triplet state. We were able to demonstrate in the middle seventies that several peroxidase-catalyzed reactions that consume oxygen generate products of the type expected from a dioxetane intermediate and that a triplet carbonyl compound is generated in high yields. These species were shown to participate in uniand bimolecular processes, thus paving the way for a "photobiochemistry without light", the biochemical conterpart of "photochemistry without light" developed by EH White and associates at Johns Hopkins University. The generation of triplet carbonyl compounds and the consequent promotion of photochemical-like processes has also been demonstrated in organized structures, e.g., organelles and even in intact cells. As a whole, the work opens the possibility of understanding the natural occurrence of certain photochemical-like processes - both useful and deleterious - in the absence of light. Several applications can be envisaged for biochemically generated triplet species.