Multi-center evaluation of oxidation-reduction potential by the MiOXSYS in males with abnormal semen / 亚洲男科学杂志(英文版)

According to the World Health Organization (WHO), oxidative stress (OS) is a significant contributor to male infertility. Seminal OS can be measured by a number of assays, all of which are either costly or time sensitive and/or require large semen volume and complex instrumentation. One less expensive alternative is to quantify the oxidation-reduction potential (ORP) with the MiOXSYS. In this international multi-center study, we assessed whether ORP levels measured by the MiOXSYS could distinguish semen samples that fall within the 2010 WHO normal reference values from those that do not. Semen samples were collected from 2092 patients in 9 countries; ORP was normalized to sperm concentration (mV/106 sperm/ml). Only those samples with a concentration >1 × 106 sperm ml-1 were included. The results showed that 199 samples fell within the WHO normal reference range while the remaining 1893 samples did not meet one or more of the criteria. ORP was negatively correlated with all semen parameters (P < 0.01) except volume. The area under the curve for ORP was 0.765. The ORP cut-off value (1.34 mV/106 sperm/ml) was able to differentiate specimens with abnormal semen parameters with 98.1% sensitivity, 40.6% specificity, 94.7% positive predictive value (PPV) and 66.6% negative predictive value (NPV). When used as an adjunct to traditional semen analysis, ORP levels may help identify altered functional status of spermatozoa caused by OS in cases of idiopathic male infertility and in male partners of couples suffering recurrent pregnancy loss, and thereby directing these men to relevant medical therapies and lifestyle modifications.

Role of inhibin B as a marker of spermatogenesis in infertile patients with varicocele

Varicocele is a state of varicosity and tortousity of the pampiniform plexus around the retrograde blood flow. There is strong association between varicocele and subfertility so treating varicocele may be helping improvement of fertility. Inhibin B levels reflect the functional state of seminiferous epithelium and have been considered as sensitive index of spermatogenesis. This study was to detect the role of serum inhibin B concentration in infertile men with varicocele before and after varicocelectomy. Twenty patients with varicocele were included in this study. Serum inhibin B, serum FSH and semen analysis were performed before and after 6 months after varicocelectomy. There was a significant increase of inhibin B levels [P < 0.01] after varicocelectomy and a significant decrease of serum FSH levels was observed after surgery P < 0.01]. A significant improvement of semen analysis, sperm concentration [P < 0.01] and progressive motility [P < 0.05] was showed after treatment. Serum Inhibin B level in infertile men may provide a useful information about spermatogenesis and could be used as a more direct marker of spermatogenesis than FSH. Moreover, varicocelectomy improves the level of inhibin B and semen parameters in those patients