Regenerated rat skeletal muscle after periodic contusions

In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 + or - 0.15 vs 0.91 + or - 0.09 g, P = 0.03; four months: 0.79 + or - 0.14 vs 1.02 + or - 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with centralized nuclei, mainly in the deep portion. Damaged muscles presented a higher incidence of undifferentiated fibers when compared to the intact one (one month: 3.4 + or - 2.1 vs 0.5 + or - 0.3 percent, P = 0.006; four months: 2.3 + or - 1.6 vs 0.3 + or - 0.3 percent, P = 0.007, respectively). Injured TA evaluated one month later showed a decreased area of muscle fibers when compared to the intact one (P = 0.003). Thus, we conclude that: a) muscle fibers were damaged mainly in the deep portion, probably because they were compressed against the tibia; b) periodic contusions in the TA muscle did not change the percentage of type I and II muscle fibers; c) periodically injured TA muscles took four months to reach a muscle fiber area similar to that of the intact muscle

Contusión pulmonar / Pulmonary contusion

La contusión pulmonar es una afección frecuente que debe sospecharse en pacientes con traumatismo torácico que cursan con hipoxemia refractaria. El diagnóstico y tratamiento tempranos disminuyen de manera considerable la morbilidad y la mortalidad. En el presente artículo se revisa, a propósito de un caso, los conceptos actuales de la contusión pulmonar, así como la fisiopatología, manifestaciones clínicas, clasificación, diagnóstico y tratamiento