Increased oxygen free radical production from isolated human pmnls and whole blood by luminol-enhanced chemiluminescence in autistic children

To investigate the estimated capacity of polymorphonuclear leucocytes [PMNLs] and whole blood to produce reactive oxygen species [ROS] in children with autism and mental retardation, and compare it with normal children. Cohort study conducted between January and April 2007. Chemiluminescence laboratory [CL], Department of Physiology, Faculty of Medicine, King Khaled University Hospital, Riyadh, Saudi Arabia. Forty autistic and eight mentally retarded children. Oxygen free radical production [O[2]-, H[2]O[2], OH-] was detected by luminol-enhanced chemiluminescence, from isolated PMNLs and whole blood, stimulated by phorbol myristate acetate [PMA] and opsinized zymazan [OPZ]. Oxygen free radical production from whole blood and PMNLs. Forty autistic [35 male and fivefemale],andeightmentally retarded children [study group] were compared with forty six normal Saudi children [control group]. The mean age was 7.4 +/- 0.5 years. The CL peak response of whole blood and PMNLs stimulated with PMA and OPZ, in autistic children was significantlyhigher [p < 0.05]compared to control children. However, the CL peak response in children with mental retardation did not show any significantdifferenceswhencomparedtothecontrolgroup. There is an increase in oxygen free radicals production from whole blood and from PMNLs in autistic children. Therefore, an increase the antioxidant consumption in autistic children is strongly recommended

Tamiz neonatal para hipotiroidismo congénito. Experiencia en el Laboratorio Regional del Sureste / Neonatal screening for congenital hypothyroidism. Experience in the Southeast Regional Laboratory

Introducción. Se presenta en este artículo los resultados del Programa para Prevención de Retraso Mental por Hipotiroidismo Congénito del Laboratorio Regional del Suereste. El objetivo del estudio fue mostrar la frecuencia de este problema en las muestras enviadas por los hospitales de Yucatán, Campeche, Quintana Roo, Chiapas, Tabasco y Oaxaca; asimismo, se describen algunos problemas y dificultades realcionados con éste. Material y métodos. Se estudiaron 58,154 muestras de sangre de niños en edades comprendidas desde el nacimiento hasta los 3 meses de edad, durante el período de enero de 1993 a diciembre de 1995. Resultados. Se detectó 20 casos de hipotiroidismo congénito, lo cual dio una incidencia de 1 caso en 2,907 recién nacidos tamizados. Conclusión. La incidencia fue menor a la reportada en estudios efectuados en México; asimismo, se identificaron algunos problemas relacionados con la toma de la muestras y los seguimientos de los casos sospechosos