Hiperplasia sebácea cutânea: estudo piloto para a correlação da doença com hormônios androgênios / Sebaceous hyperplasia: a pilot study to correlate this skin disease with circulating androgen levels

FUNDAMENTOS: As glândulas sebáceas são suscetíveis à ação dos hormônios androgênios e apresentam proliferação benigna com a idade, ou seja, hiperplasia. OBJETIVOS: Estudo piloto para verificar se há correlação entre a taxa de hormônios masculinos circulantes e o aumento da incidência da hiperplasia das glândulas sebáceas. MÉTODOS: 16 pacientes do sexo feminino, com diagnóstico de hiperplasia sebácea cutânea, foram comparados a um grupo-controle de mesmo gênero e idades semelhantes, sem a doença. Ambos os grupos foram submetidos a testes de dosagem sanguínea para avaliação das taxas de hormônios androgênios circulantes (testosterona livre e total, androstenediona). Os resultados foram tabulados e analisados estatisticamente. RESULTADOS: Os dados demonstraram não haver mudanças nos níveis de hormônios masculinos circulantes dos pacientes com hiperplasia sebácea cutânea, quando comparados ao grupo-controle. CONCLUSÃO: Os dados sugerem que não há alterações estatisticamente significantes nas taxas dos hormônios circulantes (testosterona livre e total, androstenediona, deidroepiandrosterona, sulfato de deidroepiandrosterona) dos pacientes com hiperplasia sebácea cutânea.

BACKGROUND: The sebaceous glands are susceptible to the effects of androgens. A benign proliferation of these hormones, i.e. hyperplasia, occurs with age. OBJECTIVES: This was a pilot study to demonstrate whether any correlation exists between circulating androgen levels and an increase in the incidence of sebaceous hyperplasia. METHODS: Sixteen female patients with a diagnosis of sebaceous hyperplasia were compared to a control group of females of a similar age without the disease. Blood tests were performed on participants of both groups to measure circulating androgen levels (free and total testosterone and androstenedione levels). Results were tabulated for statistical analysis. RESULTS: These data showed no statistically significant differences in circulating androgen levels between the patients with sebaceous hyperplasia and the control group. CONCLUSION: These data suggest that no significant changes occur in circulating androgen levels [free and total testosterone, androstenedione, dehydroepiandrosterone (DHEA) and DHEA sulfate] in patients with sebaceous hyperplasia.

Pesquisa de tecido prostático em pacientes 46, XX portadoras da forma clássica de hiperplasia congênita das suprarrenais / Search of prostatic tissue in 46, XX congenital adrenal hyperplasia

OBJETIVOS: Verificar a ocorrência de tecido prostático em pacientes portadoras da forma clássica de hiperplasia congênita das suprarrenais, com cariótipo 46,XX e analisar a sensibilidade e a especificidade do antígeno prostático específico (PSA) das pacientes com hiperplasia congênita das suprarrenais em relação à detecção de tecido prostático na ressonância magnética (RNM) de região pélvica. MÉTODOS: Foram estudadas 52 crianças e adolescentes, sendo 32 meninas portadoras da forma clássica de hiperplasia congênita das suprarrenais, 10 meninas e 10 meninos sem hiperplasia congênita das suprarrenais. A RNM da região pélvica e a coleta de PSA, diidrotestosterona e testosterona foram realizadas em todos os pacientes. Para analisar a capacidade de discriminação do antígeno prostático-específico, foi utilizada a curva ROC (receiver operating characteristic curve). RESULTADOS: Cinco das 32 pacientes portadoras de hiperplasia congênita das suprarrenais apresentaram tecido prostático na RNM de região pélvica. Para concentração de antígeno prostático-específico de 0,1 ng/mL, obteve-se sensibilidade de 100 por cento e especificidade de 88,9 por cento para a detecção de tecido prostático. CONCLUSÕES: A ocorrência de tecido prostático nas pacientes portadoras de hiperplasia congênita das suprarrenais estudadas foi de 15,6 por cento. O antígeno prostático-específico mostrou ser valioso marcador de tecido prostático nestas pacientes.

OBJECTIVES: To describe the presence of prostatic tissue in 46,XX patients with the classical form of congenital adrenal hyperplasia (CAH); to evaluate the sensitivity and specificity of prostatic specific antigen (PSA) measured in congenital adrenal hyperplasia patients with regard to the detection of prostatic tissue in pelvic MRI. METHODS: We studied 52 children and adolescents, 32 with the classical form of congenital adrenal hyperplasia, 10 boys and 10 girls without CAH. Pelvic MRI was performed in all patients to detect prostatic tissue. Prostate specific antigen, testosterone and dihydrotestosterone were measured in all patients. We used Receiver Operating Characteristic Curve for PSA discrimination capacity. RESULTS: Five girls with congenital adrenal hyperplasia showed image of prostatic tissue on pelvic MRI. Prostate specific antigen showed sensitivity and specificity of 100 percent and 88.9 percent, respectively, taking 0.1 ng/mL as the cutoff level. CONCLUSIONS: The incidence of prostatic tissue in 46,XX patients with the classical form of congenital adrenal hyperplasia was 15.6 percent. PSA demonstrated to be a good marker of prostatic tissue in these patients and should be used to screen patients to be submitted to image studies.

[ effects of hydro alcoholic extract of dorema aucheri on blood concentration of gonadotropin and androgen hormones in adult male rats]

Dorema aucheri [Apieaceae] contains chemical compounds including flavonoids and coumarins. Flavonoids have estrogenic properties and coumarins have antiandrogenic properties. The compounds are very effective on the HPG axis. In the present study, the effect of Dorema aucheri alcoholic extract on LH, FSH, testosterone and DHT hormones on adult male rats were determined. This was an experimental study in which male adult rats were chosen and divided into 5 groups: control group which did not received any extract, sham groups which took distilled water, experimental groups, which orally took 100, 200 and 400 mg per kg of the Dorema aucheri extract for 28 consecutive days. Then the animals were weighed and the blood sample of each group was taken and used for measuring of the serum concentration of FSH, LH, DHT and testosterone. The collected data were analyzed by the SPSS software using ANOVA and t-test. The results revealed no differences in the average weight of the body and concentration of FSH hormone in the experimental group compared with the control and sham group. However significant difference was found between the concentration of LH, testosterone and dihydrotestosterone in the experimental group compared with the rest groups. Concentration of testosterone in the minimum dosage of extracts showed significant increase while significant decrease was seen in the higher dose. Significant increase was seen in the concentration of LH in all doses. DHT serum concentration in the minimum dose showed significant decrease while significant increase was seen in higher dosage. It seems that the flavonoids compound of Dorema aucheri extract caused the LH hormone to increase prolactin. Using the extract increases the LH hormone and inhibition of aromatase and 5 alfa reductase enzymes cause the testosterone and DHT hormone to increase in higher dosage

effect of different doses of finasteride on epididymis and testosterone and DHT concentrations in rats

Finasteride is a widely used in the treatment of alopecia and prostatic hyperplasia. Our study was conducted to assess the effect of different doses of Finasteride on epididymis, testosterone and dihydrotestosterone concentrations in male Sprague-Dawley rats. This is experimental study conducted where thirty male rats are divided into three groups [10 rats in each group]; high dose, low dose and control groups. During 2 months period, testosterone and DHT were being measured and at the end of the experiment, rats were killed by cervical dislocation. Weight of the organs and histopathological examination were done. DHT was found significantly reduced by finasteride administation. Testosterone was unexpectedly reduced in our study. Reduction in glandular epithelium and stromal component was clearly evident in histopathological examination of the prostate. Weights of seminal vesicles, dorsal prostate and ventral prostate were significantly decreased with finasteride administration. Sperm count and motility were not significantly changed in low-dose finasteride-treated group but there was a significant reduction in sperm counts and motility in high-dose group. Finasteride has significant effects on testosterone, DHT. Also, it can affect epididymal functions if administered in high dose. It is evident from our results that there is a need for further clarification of the relation between finasteride administration and specific parameters in epididymal functions

Un método no cromatográfico para la determinación de dihidrotestosterona en plasma / A non-chromatographic method for the determination of plasma dihydrotestosterone

Se describe una técnica no cromatográfica para la determinación por radioinmunoensayo de dihidrotestosterona (DHT), en la cual se introduce un paso de oxidación que elimina la interferencia de la testosterona (T). El ensayo es validado y se determinan los valores normales para una población de hombres (0,82-3,56 nmol/l) y mujeres (0,33-1,13 nmol/l) sanos y fértiles. Se comparan los valores obtenidos con los informados en la literatura