Phosphate solubilizing ability of Emericella nidulans strain V1 isolated from vermicompost.

Phosphorus is one of the key factors that regulate soil fertility. Its deficiencies in soil are largely replenished by chemical fertilizers. The present study was aimed to isolate efficient phosphate solubilizing fungal strains from Eisenia fetida vermicompost. Out of total 30 fungal strains the most efficient phosphate solubilizing one was Emericella (Aspergillus) nidulans V1 (MTCC 11044), identified by custom sequencing of β-tubulin gene and BLAST analysis. This strain solubilized 13 to 36% phosphate from four different rock phosphates. After three days of incubation of isolated culture with black Mussorie phosphate rock, the highest percentage of phosphate solubilization was 35.5±1.01 with a pH drop of 4.2±0.09. Kinetics of solubilization and acid production showed a linear relationship until day five of incubation. Interestingly, from zero to tenth day of incubation, solubility of soil phosphate increased gradually from 4.31±1.57 to 13.65±1.82 (mg kg-1) recording a maximum of 21.23±0.54 on day 45 in respect of the V1 isolate. Further, enhanced phosphorus uptake by Phaseolus plants with significant pod yield due to soil inoculation of Emericella nidulans V1 (MTCC 11044), demonstrated its prospect as an effective biofertilizer for plant growth.

Reportes clínicos: infección probada por Aspergillus flavus y probable infección pulmonar por Emericella nidulans en pacientes inmunodeprimidos / Clinical reports: tested rhinosinusal infection by Aspergillus flavus and probable pulmonary infection by Emericella nidulans in immunodepressed patients

En los últimos 20 años la incidencia de aspergilosis invasiva ha aumentado entre 5 y 10 veces, presentando una mortalidad que varía desde un 60 a 98 por ciento dependiendo de su localización, enfermedad de base (inmunodepresión), diagnóstico y tratamiento precoz. A pesar que se ha logrado un importante avance en estos dos últimos decenios, aproximadamente un 50 por ciento de los casos se diagnostican post mortem. Se reportan dos casos de aspergilosis (probada y probable) en pacientes inmunodeprimidos de 21 y 44 años respectivamente. El primer caso corresponde a una infección rinosinusal en una paciente con leucemia linfoblástica, con un cuadro clínico caracterizado por aumento de volumen facial izquierdo, compromiso progresivo del estado general y equimosis en región periorbitaria. La endoscopia nasal detectó abundante secreción y tejido friable amarillento sugerente de infección micótica. Las muestras de tejidos enviadas al laboratorio para examen directo y cultivos arrojaron presencia de hifas en la mucosa y crecimiento abundante de Aspergillus flavus. Se inició terapia con itraconazol, cambiándose posteriormente a voriconazol, con respuesta favorable y disminución del dolor y volumen facial. Sin embargo, la paciente fallece al 10º día del ingreso debido a su mal estado general, avance de la falla medular y posterior insuficiencia respiratoria. El segundo caso corresponde a una probable infección pulmonar en una paciente con cáncer de mama en tratamiento quimioterápico. La paciente ingresó con el diagnóstico de neutropenia febril y probable neumonía de etiología bacteriana, fue tratada con antimicrobianos tanto en el hospital comunal como en el de base. Sin embargo, presentó una evolución tórpida, cursando posteriormente con hemoptisis, insuficiencia respiratoria y compromiso de conciencia. Se tomaron muestras de secreción endotraqueal las que fueron enviadas para estudio bacteriológico(TBC) y micológico, además de hemo y urocultivos. Los resultados de los...

In the lately twenty years the incidence of invasive spergillosis has increased five and ten times, causing a mortality rate ranging from 60 to 98 percent, depending on its location, nature of disease (immunodepression), diagnosis and forward treatment. In spite of the fact that there has been a significant progress in this lately period of twenty years, about 50 percent of cases were detected post mortem. Two cases of spergillosis (tested and probable) in 21 and 44 aged immunodepressed patients respectively are herein reported. The first case involves a rhinosinusal infection in a female patient diagnosed with lymphoblastic leukemia, whose clinical symptoms were an increase in the left volume of her face, a progressivecompromise in her overall state of health and ecchymosis in her periorbitary region. Nasal endoscopy revealed abundant secretion and yellowish friable tissues which suggested a mycotic infection. Samples of tissues sent to the lab for direct exam and cultures revealed the presenceof hypha in the mucus and an abundant growth of Aspergillus flavus. The first therapy was with itraconazol, changing later on to voriconazol what resulted in adecrease of pain and face volume. However, the patient dies on the tenth day of her ingress due to her bad state ofhealth, progress in glandular failure and ultimate respiratory insufficiency. The second case involves a probable pulmonary infection in a patient diagnosed with breast cancer and receiving chemotherapy treatment. She was ingressed with a febrile neutropenia and a probable bacterial etiology pneumonia and she was treated with antimicrobial medication both in the community hospital and in the base building. However, she showed a torpid evolution followed furthermore by hemoptisis, respiratory insufficiencyand consciousness compromise. Samples of endotraqueal secretion were taken in order to submit them to bacteriological and mycological (TBC) studies, together with hemo...