Aspectos da E-caderina na invasão óssea do carcinoma epidermóide da mucosa oral / E-cadherin expression in oral squamous cells carcinoma with boné invasion

O carcinoma epidermóide da mucosa oral (CEMO) é uma neoplasia maligna comum; no Brasil, são estimados, para 2016, 15.490 novos casos. A invasão óssea ocorre em casos avançados.; esta é classificada em erosiva e infiltrativa. Aparentemente, o processo de transição epitélio-mesenquimal, com o envolvimento da E-caderina, é implicado. Foi investigada a expressão de E-caderina, por meio da imunoistoquímica em 15 casos avançados de CEMO e avaliada sua correlação com as características clínicas e histológicas da invasão óssea. A imunoexpressão da E-caderina foi estudada nos 15 casos de CEMO com evidência histológica de invasão óssea. A maioria dos pacientes eram homens (10 pacientes) e apresentavam invasão em mandíbula (9 casos). A expressão de E-caderina foi negativa em CEMOs com invasão erosiva e positiva nos casos que apresentavam infiltração óssea. A expressão de E-caderina na invasão óssea sugere que a participação do fenômeno de transição epitélio-mesenquimal é um fator diretamente envolvido com o tipo de invasão óssea.

Oral squamous cell carcinoma (OSCC) is a common malignancy; in Brazil it is estimated, in 2016,15.490 new cases. Bone invasion occurs in advanced cases; it is classified in erosive and infiltrative patterns. Apparently, the epithelial-mesenchymal phenomenon, with important participation of E-cadherin is implicated. We investigated the expression of E-cadherin in advanced OSSC and correlated its expression with the clinical characteristics and histologic patterns of bone invasion. Immunoexpression of E-cadherin was studied in 15 cases of OSCC with histological evidence of bone invasion. Most patients were men (10 patients) and presented mandible invasion (9 cases). The expression of E-cadherin was negative in OSCC in erosive bone invasion and positive in the infiltrative bone invasion. E-cadherin expression in bone invasion suggests that participation of epithelial-mesenchymal phenomenon is dependent on the patterns of tumour bone invasion.

Morphology and histological annual changes of the oviduct of chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae) / Morfología y cambios histológicos anuales del oviducto de Chaetophractus villosus (Mammalia, Xenarthra, Dasypodidae)

The morphological features of the oviduct in the armadillo Chaetophractus villosus (Desmarest, 1804) were studied. In the simple columnar epithelium, ciliated cells, secretory cells, and cells of the bottom of the folds are described. The most remarkable findings are the increase in number of ciliated cells, when present, from the fimbriae to the uterus, being the isthmus practically lined by ciliated cells, and that secretory cells are more abundant in the apical zone of the ampulla and fimbriae, than in the isthmus. Morphological changes along the year in the different portions are described.

Se estudiaron las características morfológicas del oviducto del armadillo Chaetophractus villosus (Desmarest, 1804). En el epitelio, columnar simple, se describen células ciliadas, secretoras y células del fondo de los pliegues. Las células ciliadas, cuando están presentes, aumentan en número desde las fimbrias hacia el útero, estando el istmo prácticamente tapizado por células ciliadas, las células secretoras son más abundantes en la zona apical de la ampolla y en las fimbrias, que en el istmo. Se describen los cambios morfológicos en las diferentes porciones a lo largo del año.

Ultrastructural developmental study of bronchial epithelium in one-humped camel

The putative respiratory epithelium lining of the bronchial tree of 20-60 mm crown-rump length [CRL] camel fetuses displayed the staggered nuclear organization characteristic of single cilium on their apical surfaces. The bronchial epithelium revealed a clear evidence of ciliogenesis, which occurred as early as 70 mm CRL, starting in the main bronchi and gradually proceeding distally, along the developing bronchial tree. The ciliogenic cells were gradually increased with gestational age. in fetuses of 70-370 mm CRL, the bronchial epithelium displayed a myriad of disintegrating sloughed apoptotic cells intermingled with ciliated and ciliogenic cells together with few non-ciliated microvillus types. Mitochondrial swelling, dilatation of the rough endoplasmic reticulum, vacuolation of the of the cytoplasm, distortion of the intercellular junctions, together with clumping of the nuclear chromatin were the early signs of cellular apoptosis. With time, the regressing apoptotic cells tended to detach themselves from their neighbors and underlying basement membrane moved upwards and sloughed into the airway lumen. These morphological alterations, concerning the disintegrating sloughed apoptotic cells, were first observed in the main bronchi and gradually proceeded by age towards the bronchiolar branches. In adult camels the bronchial epithelium was formed mainly of ciliated and non-ciliated microvillus cells; however, that at term of gestation was composed of ciliated cells, non-ciliated microvillus cells; however, that at term of gestation was composed of ciliated cells, non-ciliated microvillus types together with some cells possessing surface microplicae

In vitro explant culture of mantle epithelium of freshwater pearl mussel.

The in vitro culture of nacre secreting pallial mantle explants of freshwater pearl producing mussel, Lamellidens marginalis (Lamarck) included depuration of pearl mussels with different physical and chemical agents to eradicate various commensals, removal of pallial mantle ribbon, aseptic preparation of explants from the ribbon and transfer of those explants into tissue culture petri dishes. Special synthetic tissue culture media enriched with additives viz., inactivated calf fetal serum and antibiotics were poured into plates with explants. The culture plates were incubated at 30 degrees C in a CO2 incubator at 5%, CO2. The cultures could be maintained for 42-45 days without any contamination. After 12 hr epithelial like cells began to migrate out and formed a complete cell sheet surrounding the explant within 12-15 days. The epithelial cells in the culture indicated functional viability as subsequently after 38-40 days of culture, typical aragonitic 'nacre' crystals of CaCO3 could be observed throughout the culture plates.

Epithelial invasion of anterior chamber after cataract extraction

Epithelial down growth must be avoided by a good wound closure with good cleaning of its edges. If a fistula appears in spite of those precautions, the plug graft is shown to be the most efficient treatment. If an advanced epithelial down growth is evident a large excision of the invaded tissues must be performed and a keratoplasty with repair of the area lost. An early diagnosis which imposes an urgent surgical decision is an essential point of the prognosis