Cinética de desialización eritrocitaria producida por larvas musculares vivas de Trichinella spiralis / Erythrocyte desialylation kinetics produced by living Trichinella spiralis muscle larvae / Cinética de dessialização eritrocitária causada por larvas musculares vivas da Trichinella spiralis

Las larvas musculares (LM) de T. spiralis alteran la agregación eritrocitaria. El objetivo del trabajo fue estudiar la cinética de desialización eritrocitaria producida por LM vivas de T. spiralis. Se realizaron 3 experiencias en las que se incubaron 60 larvas con 30 μL de eritrocitos en 1 mL de solución salina durante 1, 2, 3, 4, 5, 6, 7, 10, 15, 18, 20, 22 y 24 horas. Se aplicó el Método de Titulación de la Agregación por Polibrene y se calculó Título, Score Total y CexpCASP en los eritrocitos Control e incubados con LM. Los resultados mostraron que en la primera hora no hubo captación de ácido siálico. A las 2 horas el coeficiente comenzó a decrecer y a las 3 horas el Título disminuyó en una dilución y el coeficiente fue 0,62±0,021. En los siguientes tiempos el Título se mantuvo y el valor del coeficiente presentó pequeñas disminuciones, hasta alcanzar el valor de 0,45±0,010 a las 22 horas, tiempo en que se produjo la disminución significativa del Título. A las 24 horas hubo una nueva disminución del Título del Control y CexpCASP fue 0,13±0,093. Se concluye que las LM vivas durante incubación in vitro con eritrocitos comenzarían a captar ácido siálico a partir de las 2 horas de contacto logrando la desialización casi completa del eritrocito a las 24 horas.

T. spiralis muscle larvae (ML) alter erythrocyte aggregation. The objective of this work was to study erythrocyte desialylation kinetics produced by living T. spiralis ML. Three experiments were conducted in which 60 larvae were incubated with 30 μL of erythrocytes in 1 mL of saline solution for 1, 2, 3, 4, 5, 6, 7, 10, 15, 18, 20, 22 and 24 hours. Titration of Aggregation by Polybrene Method was used and Title, Total Score and CexpCASP were calculated in Control erythrocytes and erythrocytes incubated with ML. The results showed that in the first hour there was no capture of sialic acid. The coefficient began to decrease at 2 hours, and at 3 hours the Title decreased in one dilution and the coefficient was 0.62±0.021. The title was maintained at the following times and the coefficient value presented small decreases, until reaching 0.45±0.010 value at 22 hours. It was then that, a significant decrease in Title occurred. Within 24 hours, there was a further decrease of Control Title, and CexpCASP was 0.13±0.093. It can be concluded that living ML during in vitro incubation with erythrocytes began to capture sialic acid after 2 hours of contact, getting almost complete desialylation of erythrocytes at 24 hours.

As larvas musculares (LM) de T. spiralis alteram a agregação eritrocitaria. O objetivo do trabalho foi estudar a cinética de dessialização eritrocitária produzida por LM vivas de T. spiralis. Realizaram-se 3 experiências nas quais foram incubadas 60 larvas com 30 μL de eritrócitos em 1 μL de solução salina durante 1, 2, 3, 4, 5, 6, 7, 10, 15, 18, 20, 22 y 24 horas. Foi aplicado o Método de Titulação da Agregação por Polibreno e se calculou Título, Pontuação Total (ST) e CexpCASP. Os resultados mostraram que na primeira hora não houve captura de acido siálico. Às 2 horas o coeficiente começou a decrescer e às 3 horas o Título diminuiu numa diluição e o coeficiente foi 0,62±0,021. Nos tempos seguintes o Título se manteve e o valor do coeficiente apresentou pequenas diminuições, até atingir o valor de 0,45±0,010 às 22 horas, tempo em que se produziu a diminuição significativa do Título. Às 24 horas houve uma nova diminuição do Título do Controle e o CexpCASP foi 0,13±0,093. Conclui-se que as LM vivas durante incubação in vitro com eritrócitos, começariam a captar ácido siálico a partir das 2 horas de contato conseguindo a dessialização quase completa do eritrócito às 24 horas.

Red blood cell elution time of strains of Newcastle disease virus

Elution time of velogenic, mesogenic and lentogenic strains of Newcastle disease virus was determined. The differences in their elution time were also calculated. Four samples, each of a velogenic strain (VGF2), a mesogenic strain (Komarov) and a lentogenic strain (LaSota) were used for hemagglutination test with 0.6% chicken red blood cells. The time it took for wells of the end hemagglutination points (highest dilution that gave agglutination) to elute was recorded as elution time for each sample. The mean elution time of the three strains of Newcastle disease virus differed significantly (p < 0.05). The velogenic strain gave the highest mean elution time of 118 min, followed by the mesogenic strain with 59 min and the lentogenic strain with 25 min. Based on this result it appears that elution time could form a basis for rough characterization of isolates of Newcastle disease virus into the three major strains.