Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Adicionar filtros








Intervalo de ano
1.
Acta cient. venez ; 39(2): 140-6, 1988. ilus
Artigo em Inglês | LILACS | ID: lil-74771

RESUMO

Ketoconazole, a dioxolane imidazole, affects growth and sterol synthesis by Trypanosoma (Schizotryparum) cruzi epimastigotes in a time - and concentration - dependent manner. Starting with a cell density of 10 7 cells/ml, a 10 -4M concentration of the drug blocks instantaneously growth, but with 10 -5M and 10 -6M growth arrest takes place at 48 and 96 hours, respectively. A study of the sterol content of the parasites and their de novo synthesis reveals that the drug induces the accumulation of trimethyl- and, to a much lesser extent, dimethyl-sterols with a progressive decrease of ergosterol-like desmethyl-sterol pool. Sterols added to the growth medium can partially reverse the growth inhibitory effects of ketoconazole, ergosterol being much more effective that cholesterol in this action. These facts suggest an essential function performed in T cruzi by ergosterol, which cannot be replaced by cholesterol, a compound which is passively absorbed from the growth medium and remains in the drug-treated cells. At 10 -6M ketoconazole blocks completely the incorporation of 14C-acetate in desmethyl-sterols and >97% of the radioactivity appears in the trimethyl-sterol fraction at 24 hours; further incubation leads to the appearance of a slight amount of radioactivity in the dimethyl-sterol fraction (10% at 96 hours)..


Assuntos
Cetoconazol/farmacologia , Esteróis/efeitos dos fármacos , Trypanosoma cruzi/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA