RESUMO
OBJECTIVE@#To study the relationship between DNA degradation and postmortem interval of corrupt corpse.@*METHODS@#By determining the marrow DNA content with histochemical technique and image analysis.@*RESULTS@#The content of marrow DNA decreased gradually with prolongation of postmortem interval, and it evencould be detected till 14 days after death.@*CONCLUSION@#There was a linear relationship between the degradation rate of the nuclear DNA and postmortem interval.
Assuntos
Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Células da Medula Óssea/metabolismo , Cadáver , Núcleo Celular/metabolismo , DNA/metabolismo , Patologia Legal/métodos , Processamento de Imagem Assistida por Computador , Mudanças Depois da Morte , Análise de Regressão , Coloração e Rotulagem , Esterno/citologia , Fatores de TempoRESUMO
The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the expression of the other markers in the normal BM, or in any of the markers in the leukemic BM. Based on the literature, we set the cut-off value for the normal CD10 expression at 45 x 10Ý a.u. for both age groups. For the remaining markers we established the cut-off values based on the minimum-maximum values in the normal BM in each age group. The expression of CD10 was higher than the cut-off in 30 ALL cases and in 18 of them there was a concomitant aberrant expression of other markers. In 9 of the 10 CD10+ ALL with normal CD10 SABC values, the expression of at least one other marker was aberrant. In conclusion, the distinction between normal and leukemic cells by QFC was possible in 38/40 CD10+ ALL cases