Induction, production and purification of streptolysin S. [SLS]

Strain C203S of Streptococcus pyogenes group A was examined for the production of Streptolysin S [SLS]. The strain under investigation produced the highest yield of Streptolysin S. The combined material [crude SLS] was further purified by hydroxylapatile column chromatography. The purified product had a specific activity 3.5 x 10[5] haemolytic units [mg protein]-1