RESUMO
OBJECTIVE@#To develop the test Paper of gold-labelled antibody against fibronectin EIIIR A splicing variant, which can be available in forensic wound interval estimation.@*METHODS@#Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats.@*RESULTS@#On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining.@*CONCLUSION@#The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.
Assuntos
Animais , Ratos , Processamento Alternativo , Anticorpos Monoclonais , Ensaio de Imunoadsorção Enzimática , Fibronectinas/metabolismo , Patologia Legal , Coloide de Ouro , Imunoensaio/métodos , Distribuição Aleatória , Ratos Sprague-Dawley , Fitas Reagentes , Pele/metabolismo , Fatores de Tempo , Ferimentos não Penetrantes/metabolismoRESUMO
OBJECTIVE@#To evaluate changes of VEGF in skin after blunt injury.@*METHODS@#The rats of injury groups were subjected to skin blunt injury by free-falling iron hammer. The samples taken at 1 h, 3 h, 6 h, 12 h, 1 d, 3 d, 5 d, 7 d, 9 d after injury were studied by immunohistochemistry and MIAS image analysis system.@*RESULTS@#In the skin of normal control group the expression level of VEGF was low. The increase of VEGF could be observed at 1 day after injury, reached peak at 7 days and declined at 9 days.@*CONCLUSION@#Blunt injury in the skin could induce the expression of the VEGF. Moreover, the change pattern of VEGF level was quite regular with time and could be used to estimate the time after skin injury.