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1.
São Paulo; s.n; s.n; 2023. 135 p. tab, graf.
Tese em Inglês | LILACS | ID: biblio-1437852

RESUMO

Proteins equipped with flavin adenine dinucleotides (FAD) or flavin mononucleotides (FMN) are named flavoproteins and constitute about 1% of all existing proteins. They catalyze redox, acid-base and photochemical reactions in a variety of biochemical phenomena that goes from energy metabolism to DNA repair and light sensing. The versatility observed in flavoproteins is ultimately a balance of flavin intrinsic properties modulated by a protein environment. This thesis aims to investigate how flavoproteins work by systematic evaluating flavin properties and reactivity. In particular, the mechanism of fumarate reduction by the flavoenzyme fumarate reductase Fcc3 was determined. Electronic-structure calculations were used for this task based on rigorous calibration with experimental data and error assessment. Flavin properties at chemical accuracy were obtained with single reference coupled-cluster CCSD(T) calculations at the complete basis set limit. Density functional theory was demonstrated an excellent alternative with lower computational costs and slightly less accuracy. Flavin protonation and tautomerism were shown to be important modulators of flavin properties and reactivity, with the possibility of various tautomers existing at neutral pH. Regarding flavin redox properties, an analysis based on multiconfigurational wave function weights was proposed for categorizing flavin redox reactions as hydride or hydrogen-atom transfers. This analysis is an upgrade over traditional partial charges methods and can be applied not only to flavin reactions but to any protoncoupled electron transfer. In the investigation of the enzymatic mechanism of fumarate reduction, the reaction was determined as a nucleophilic addition by hydride transfer with carbanion formation. Fumarate reductase employs electrostatic catalysis in contrast to previous proposals of substrate straining and general-acid catalysis. Also, hydride transfer was shown to be vibronically adiabatic with low tunneling contribution. These findings give new insights into the mechanisms of fumarate reductases and provide a framework for future computational studies of flavoproteins in general. The analyses and benchmark studies presented can be used to build better models of properties and reactivity of flavins and flavoproteins


Proteínas equipadas com dinucleotídeos de flavina-adenina (FAD) e mononucleotídeos de flavina (FMN) são chamadas flavoproteínas e constituem cerca de 1% de todas as proteínas existentes. Elas catalisam reações redox, ácido-base e fotoquímicas numa variedade de fenômenos bioquímicos que vão desde o metabolismo energético até reparo de DNA e captação de luz. A versatilidade observada em flavoproteínas é em última instância um balanço das propriedades intrínsecas de flavinas moduladas por um ambiente proteico. Esta tese busca investigar como flavoproteínas funcionam através de avaliações sistemáticas de propriedades e reatividade de flavinas. Em particular, o mecanismo de redução de fumarato pela flavoenzima fumarato redutase Fcc3 foi determinado. Cálculos de estrutura eletrônica foram usados para esta tarefa com base em rigorosa calibração com dados experimentais e avaliação de erros. As propriedades de flavinas foram determinadas com acurácia química com cálculos monoconfiguracionais de coupled-cluster CCSD(T) no limite de conjunto base completo. A teoria do funcional da densidade mostrou-se uma alternativa excelente com menor custo computacional e um pouco menos de acurácia. Protonação e tautomerismo de flavinas mostraram-se moduladores importantes de suas propriedades e reatividade, com a possibilidade de vários tautômeros existirem em pH neutro. Em relação às propriedades redox de flavinas, uma análise baseada nos pesos de funções de onda multiconfiguracionais foi proposta para categorizar as reações redox de flavinas como transferências de hidreto ou hidrogênio. Esta análise é uma melhoria em relação aos métodos tradicionais de cargas parciais e pode ser aplicada não apenas para reações de flavinas mas para qualquer transferência de próton acoplada a elétrons. Na investigação do mecanismo enzimático de redução de fumarato, a reação foi designada como uma adição nucleofílica por transferência de hidreto e formação de carbânion. A fumarato redutase usa catálise eletrostática diferentemente de prospostas anteriores envolvendo distorção do substrato e catálise ácida geral. Além disso, a transferência de hidreto mostrou-se vibronicamente adiabática com pouca contribuição de tunelamento. Estas descobertas abrem novas perspectivas sobre os mecanismos de fumarato redutases e fornecem uma base para estudos computacionais futuros sobre flavoproteínas em geral. As análises e estudos comparativos apresentados podem ser usados para construir melhores modelos para propriedades e reatividade de flavinas e flavoproteínas


Assuntos
Estudo Comparativo , Flavinas/análise , Flavoproteínas/análise , Cálculos/química , Eletricidade Estática/efeitos adversos , Fumaratos
2.
Protein & Cell ; (12): 780-790, 2010.
Artigo em Inglês | WPRIM | ID: wpr-757441

RESUMO

Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for treatment of inflammatory bowel disease, releasing the active component 5-aminosalycilic acid. The bacterium P. aeruginosa has three azoreductase genes, paAzoR1, paAzoR2 and paAzoR3, which as recombinant enzymes have been shown to have different substrate specificities. The mechanism of azoreduction relies upon tautomerisation of the substrate to the hydrazone form. We report here the characterization of the P. aeruginosa azoreductase enzymes, including determining their thermostability, cofactor preference and kinetic constants against a range of their favoured substrates. The expression levels of these enzymes during growth of P. aeruginosa are altered by the presence of azo substrates. It is shown that enzymes that were originally described as azoreductases, are likely to act as NADH quinone oxidoreductases. The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution.


Assuntos
Benzoquinonas , Metabolismo , Domínio Catalítico , Estabilidade Enzimática , Evolução Molecular , Flavinas , Química , Temperatura Alta , Cinética , Mesalamina , Química , NAD , Metabolismo , NADH NADPH Oxirredutases , Química , NADP , Metabolismo , Concentração Osmolar , Oxirredução , Fenil-Hidrazinas , Química , Filogenia , Ligação Proteica , Pseudomonas aeruginosa , Espectrofotometria Ultravioleta
3.
Journal of Taibah University Medical Sciences. 2008; 3 (1): 7-15
em Inglês | IMEMR | ID: emr-88149

RESUMO

Evaluation of antimicrobial activity of Ephedra alata male plant against bacteria, fungi, yeast, and fungi; and tentative identification of active compounds related to its antimicrobial activity. Four bacteria, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli and four fungi, Aspergillus fumigatus, Penicillium italicum, Syncephalastrum racemosum, and Candida albicans were used as test microorganisms. The stem of E. alata was extracted by different solvents. Thin layer chromatography was used for the separation of active constituents whereas mass spectrometer analysis was used for the identification of major fractions constituents. Among all solvents tested, acetonitrile extracts exhibited the most potent antimicrobial effect with a broad spectral range. Thin layer chromatographic separation of active constituents in acetonitrile extracts revealed the presence of seven fractions. All fractions showed antimicrobial activities with four fractions having a potent inhibitory effect. Two fractions out of the four were successfully identified using mass spectrophotometer analysis. The tentative identification revealed two compounds having an aromatic nature and were identified as benzo [g] pteridine -8-hydroxy-7,10- dimethyl -2,4 dione [previously reported in plants] and 1,2-benzenedicarboxylic dinonyl ester [firstly reported natural occurrence, and thus we suggested calling it Alatine [1]. Alatine [1] with its broad antimicrobial spectrum could represent a good candidate for further microbiological and chemical analysis, and E. alata plant can be further subjected to isolation of the therapeutic compounds and further pharmacological evaluation


Assuntos
Fitoterapia , Anti-Infecciosos , Plantas Medicinais , Acetonitrilas , Flavinas
5.
Indian J Biochem Biophys ; 1989 Jun; 26(3): 186-9
Artigo em Inglês | IMSEAR | ID: sea-28070

RESUMO

Although UVA (320-400 nm) is considered less harmful to skin as compared to UVB (290-320 nm) and UVC (200-290 nm) radiation, certain endogenous chromophores may enhance UVA-induced cutaneous reactions by largely O2-dependent photodynamic reactions. Photodegradation pattern and singlet oxygen (1O2), superoxide anion radical (O2-.) producing capacity of riboflavin (RF), lumiflavin (LF) and lumichrome (LC) were examined to assess their phototoxic potential under UVA. Photolysis of RF upon exposure to UVA, UVB or UVC revealed considerable degradation to LF and LC with a near identical spectral pattern of photodegradation between 250-500 nm. Both LF and LC were stable to UVA (3 J/cm2) and UVB (400 mJ/cm2), whereas RF was photodegraded by 30 and 20%, respectively, under similar irradiation conditions. UVA-sensitized LF and LC respectively, produced nearly 15% higher and 60% lower yield of 1O2 in comparison to RF, whereas, O2-. was generated predominently by RF. Both RF and LF thus appeared to be potential chromophores for evoking deleterious effects of UVA in normal human skin.


Assuntos
Flavinas/metabolismo , Radicais Livres , Oxigênio/metabolismo , Fotólise , Riboflavina/metabolismo , Raios Ultravioleta
6.
Bulletin of Alexandria Faculty of Medicine. 1988; 24 (1): 243-7
em Inglês | IMEMR | ID: emr-120461

RESUMO

This study was carried out on 150 patients suffering from hemorrhoids to evaluate the effect of disomine on hemorrhoids in the pre- and postoperative periods. The clinical and histopathological results proved the phlebotomic activity of the drug and its value in reducing congestion and edema. The study proved the value of disomine as a useful drug when used before hemorrhoidectomy and its value as a substitute for surgery when the latter is contraindicated. No side effects of the drug had been recorded


Assuntos
Flavinas/farmacologia
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