RESUMO
OBJECTIVE@#PGM1 genotyping by PCR-SSCP analysis.@*METHODS@#Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.@*RESULTS@#2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.@*CONCLUSION@#PGM1 system typed by PCR-SSCP is useful for forensic identification.
Assuntos
Humanos , Alelos , Povo Asiático/genética , China , DNA/genética , Frequência do Gene , Genótipo , Fosfoglucomutase/genética , Reação em Cadeia da Polimerase/métodos , Polimorfismo Genético , Polimorfismo Conformacional de Fita Simples , Sensibilidade e EspecificidadeRESUMO
The genetic composition of a group of 24 Yamana indians that survive in Puerto Williams, navarino Island, Chile (parallel 55 south of Tierra del Fuego), was studied. Results showed that these indians have a different genetic composition than Pehuenche indians, specially for HLA system and sterase D. This fact validates the hypothesis based on archeological and antropological evidence, about the paleoindian origin of Yamanas