High-performance liquid chromatographic analysis of sulfur mustard reaction with amino acids and proteins

The interaction of sulfur mustard with aminoacids and proteins has been investigated in this study. Rats were injected with sublethal doses of sulfur mustard subcutaneously and intraperitoneally. At different time intervals, plasma and urine samples were collected. The binding affinity of sulfur mustard with urinary and plasma proteins and enzymes was studied for the first time using non-ideal size exclusion high-performance liquid chromatography [HPLC]. We observed that sulfur mustard reacts with proteins and enzymes containing cystein, arginine, and lysine residues