RESUMO
The present study was conducted to correlate the biotypes of Gardnerella vaginalis strains isolated from cases of bacterial vaginosis and their virulence factors. Thirty-two strains of G. vaginalis isolated from cases of bacterial vaginosis were biotyped. Adherence to vaginal epithelial cells, biofilm production, surface hydrophobicity, phospholipase C and protease activity were tested on these isolates. Biotype 1 was the most prevalent (8; 25%), followed by biotype 2 (7; 21.9%) and biotypes 5 and 8 (5; 15.6%). We did not find any statistical correlation between G. vaginalis biotypes and its virulence factors. Virulence factors expressed by G. vaginalis were not associated with a single biotype.
Assuntos
Adulto , Aderência Bacteriana , Técnicas de Tipagem Bacteriana , Biofilmes/crescimento & desenvolvimento , Células Epiteliais/microbiologia , Feminino , Gardnerella vaginalis/química , Gardnerella vaginalis/classificação , Gardnerella vaginalis/isolamento & purificação , Gardnerella vaginalis/patogenicidade , Humanos , Interações Hidrofóbicas e Hidrofílicas , Peptídeo Hidrolases/metabolismo , Fosfolipases Tipo C/metabolismo , Vaginose Bacteriana/microbiologia , Fatores de Virulência/genéticaRESUMO
Se determinaron los biotipos y susceptibilidad a ampicilina, clindamicina, eritromicina, ciprofloxacina y tetraciclina de 63 cepas de Gardnerella vaginalis aisladas de pacientes con vaginosis bacteriana. Las cepas se distribuyeron en los 8 biotipos del esquema de clasificación de Piot, siendo los biotipos 1 y 2 los más frecuentes (44,4 por ciento). Todas las cepas fueron susceptibles a ampicilina y resistentes a ciprofloxacina. Cuatro cepas (6,4 por ciento) presentaron resistencia a clindamicina y dos (3,2 por ciento) a eritromicina. Treinta y una cepas (49,2 por ciento) fueron resistentes a tetraciclina. Se estudió la base genética de la resistencia a tetraciclina en ocho cepas resistentes, demostrándose la presencia del determinante tet (M) en todas ellas.
Assuntos
Feminino , Humanos , Técnicas de Tipagem Bacteriana , Gardnerella vaginalis/isolamento & purificação , Gardnerella vaginalis/classificação , Gardnerella vaginalis , Vaginose Bacteriana/microbiologia , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos , Testes de Sensibilidade MicrobianaRESUMO
The incidence and prevalent biotypes of G. vaginalis in patients with non-specific vaginitis from Bombay, was studied. Of 300 patients screened, 105 were diagnosed to have nonspecific vaginitis (NSV). G. vaginalis was isolated from 71 per cent patients with NSV; 34.6 and 29.3 per cent G. vaginalis were belonging to biotypes 5 and 1 respectively. In 55 per cent patient, G. vaginalis was associated with anaerobes. None of the isolated strains of G. vaginalis was sensitive to 5 micrograms metronidazole disc whereas 93 per cent of the strains were sensitive to 50 micrograms metronidazole disc.