RESUMO
This study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively stained, probably because of their high compaction. In the tail, there is no paracrystalline core and the axoneme presented a very specific reaction for basic proteins. The lacinate and reticular appendages are composed of cylindrical sub-units and presented a light reaction to E-PTA and a strong reaction to tannic acid. A complex proteic coat also covers the extratesticular spermatozoa. We found similarities between both extratesticular coats, indicating a possible common origin. Both spermatozoon types are rich in proteins, especially the eupyrene appendages and the extratesticular coats. We believe that both coats are related to the sperm maturation and capacitation processes.
Assuntos
Masculino , Animais , Espermátides/química , Espermatozoides/química , Borboletas/citologia , Borboletas/química , Borboletas/ultraestrutura , Proteínas de Insetos/análise , Acrossomo/química , Acrossomo/ultraestrutura , Centríolos/química , Centríolos/ultraestrutura , Cauda do Espermatozoide/química , Cauda do Espermatozoide/ultraestrutura , Espermátides/ultraestrutura , Espermatozoides/ultraestrutura , Núcleo Celular/química , Núcleo Celular/ultraestrutura , Coloração e Rotulagem , Testículo/citologia , Testículo/química , Ducto Deferente , Glândulas Seminais/citologia , Glândulas Seminais/químicaRESUMO
Oral administration of 20, 40, 60, mg of dry Azadirachta indica leaf powder for 24 days resulted in decrease in the weights of seminal vesicles and ventral prostate, reduction in epithelial height, nuclear diameter and the secretory material in the lumen. Biochemically, there was a decrease in total protein, acid phosphatase activities. Seminal vesicles and ventral prostate being androgen dependent, the regressive changes histologically as well as biochemically, suggests the antiandrogenic property of the neem leaves.
Assuntos
Fosfatase Ácida/metabolismo , Administração Oral , Fosfatase Alcalina/metabolismo , Animais , Glucose/metabolismo , L-Lactato Desidrogenase/metabolismo , Masculino , Folhas de Planta/química , Plantas Medicinais , Pós/farmacologia , Próstata/citologia , Ratos , Glândulas Seminais/citologiaRESUMO
Investigou-se a resposta morfológica da vesícula seminal do rato adulto ao excesso de testosterona. No período compreendido entre 24 h e 14 dias de tratamento com hCG, os níveis plasmáticos de testosterona exibiram aumento progressivo de duas a cinco vezes os valores basais, enquanto a altura das células secretoras da vesícula seminal apresentou aumento constante de cerca de 20%