RESUMO
BACKGROUND & OBJECTIVES: Haemophilus influenzae causes a variety of life threatening infections in humans. Early detection of antimicrobial resistance is of importance in the treatment and management of infection. The modified Slack's method, a simple assay, has been evaluated in this study for the early detection of chloramphenicol resistance. METHODS: Fifty isolates of H. influenzae from invasive and non-invasive sites were included. Antimicrobial susceptibility testing was done by disc diffusion method and minimum inhibitory concentration (MIC) determination was performed for chloramphenicol only. Modified Slack's method was used to test for the production of chloramphenicol acetyl transferase (CAT). RESULTS: Invasive isolates showed higher degree of resistance to chloramphenicol (72%) compared to non-invasive ones (28%). One hundred per cent association was found between results of disc diffusion, MIC and CAT production amongst strains resistant to chloramphenicol. INTERPRETATION & CONCLUSION: The findings suggested that chloramphenicol still remains the drug of choice for treatment for non-invasive infection caused by H. influenzae. Modified Slack's method is a simple, rapid, inexpensive and reliable method for the detection of chloramphenicol resistance amongst H. influenzae.
Assuntos
Adulto , Idoso , Pré-Escolar , Cloranfenicol O-Acetiltransferase/análise , Resistência ao Cloranfenicol , Haemophilus influenzae/química , Humanos , Lactente , Testes de Sensibilidade Microbiana , Pessoa de Meia-IdadeRESUMO
Caracteriza 271 cepas invasivas de H. influenzae entre diciembre de 1998 a junio de 2003, aisladas de muestras tales como hemocultivos(hemo) liquido cefolorraquídeo (LCR) y líquido pleural (LP) tomadas a pacientes de hasta 5 años de edad de centros hospitalarios integrantes de la red de vigilancia centinela en Paraguay