RESUMO
Background: Sexually transmitted infections (STIs) affect sexual and reproductive health of millions of men. Pathogens such as human papillomavirus (HPV), herpes simplex virus type 1 and 2 (HSV-1 y HSV-2), Chlamydia trachomatis,Mycoplasmagenitalium,Mycoplasma hominis and Ureaplasma urealyticum are associated with STIs. Aim: To detect pathogens associated with STIs in symptomatic men and its relationship with sexual behavior. Methodology: DNA was obtained from exfoliated cells of penis from 20 symptomatic men. Pathogens were detected using qPCR or PCR followed by reverse line blot. Sexual behavior was evaluated through a survey. Results: Two or more infectious agents were detected in 50% of samples. U. urealyticum was found in 25%, meanwhile C. trachomatis and M. hominis were detected in 15%. VHS-1, VHS-2 andM. genitalium were detected only in 5%. HPV was found in all samples. The most frequent HPV genotypes were VPH 16, 11, 70. There were no statistical link found between sexual behavior and the studied microorganisms Conclusion: Infectious agents associated with STIs were detected in symptomatic men. HPV was the most frequent pathogen and it was detected in multiple genotypes. It is necessary to increase the sample size to associate significantly the sexual behavior with the results.
Introducción: Las infecciones de transmisión sexual (ITS) afectan la salud sexual y reproductiva de millones de hombres. Patógenos como virus papiloma humano (VPH), virus herpes simplex (VHS-1 y VHS-2), Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis y Ureaplasma urealyticum están asociados a ITS. Objetivo: Detectar patógenos asociados a ITS en hombres sintomáticos y relacionarlos con su conducta sexual. Metodología: Se obtuvo ADN de exfoliado celular del pene de 20 hombres sintomáticos de ITS. Los patógenos fueron detectados por RPC cuantitativa o RPC seguida de reverse line blot. La conducta sexual se evaluó mediante una encuesta. Resultados: En 50% de las muestras se detectaron dos o más agentes infecciosos; U. urealyticum fue detectado en 25% de los casos, mientras que C. trachomatis y M. hominis en 15%. VHS-1, VHS-2 y M. genitalium sólo en 5%. VPH se encontró en todas las muestras y los genotipos más frecuentes fueron VPH 16, 11, 70. No se encontró relación estadística entre los microorganismos estudiados y la conducta sexual de los encuestados. Conclusión: Se detectaron agentes infecciosos asociados a ITS en hombres sintomáticos, siendo VPH el más frecuente y encontrándose en múltiples genotipos. Es necesario aumentar el tamaño de muestra para asociar significativamente la conducta sexual a los resultados.
Assuntos
Humanos , Masculino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Comportamento Sexual/estatística & dados numéricos , Ureaplasma/genética , Infecções Sexualmente Transmissíveis/microbiologia , Infecções Sexualmente Transmissíveis/virologia , Chlamydia trachomatis/genética , Herpes Simples/genética , Mycoplasma/genética , Ureaplasma/isolamento & purificação , Chlamydia trachomatis/isolamento & purificação , Reação em Cadeia da Polimerase , Mycoplasma/isolamento & purificaçãoRESUMO
En los últimos años el gran avance de la biología molecular aplicada al diagnóstico de agentes infecciosos ha dado como resultado nuevas metodologías, más sensibles, específicas y rápidas, al alcance del médico clínico. Para el diagnóstico de los virus herpes simplex (HSV), los métodos de detección genómica y las nuevas técnicas específicas de serología han cubierto nuevas posibilidades de confirmación etiológica frente a la sospecha clínica, todo lo cual resulta finalmente en un mejor manejo terapéutico y epidemiológico.
In recent years, the great advances in the field of molecular biology, applied to diagnosis of infectious agents have resulted in new, more sensible, specific and faster methods that are more accessible to the physician. In the case of herpes simplex virus (HSV), genomic methods and specific serology have opened new possibilities for etiologic confirmation, resulting in better therapeutic and epidemiological management.
Assuntos
Humanos , Herpes Simples/genética , Herpes Simples/imunologia , Simplexvirus/genética , Simplexvirus/imunologia , DNA Viral , Herpes Simples/diagnóstico , Reação em Cadeia da Polimerase , Sorologia , Simplexvirus/isolamento & purificaçãoRESUMO
Two herpes simplex viral serotypes, HSV type and HSV type 2 may cause genital herpes. The aim of this study was to perform a genetical analysis and characterization of virus isolated from 4 patients with double genital herpetic infections. In 11 viral isolated, the cytopathic effect in Vero cells was studied, the antigenic type was determined using monoclonal antibodies and genomic analysis was performed with Eco RI, Hind III and Bgl II enzymes. Five viral isolates generated a diffuse and 6 a localized cytopathic effect. Monoclonal antibodies identified four HSV-1 and seven HSV-2. Genomic analysis had concordant results. Four HSV-1 were obtained, with different genomic patterns within them; 3 were different to the standard North American strain. The seven HSV-2 obtained had 3 different types of electrophoretic profiles, thet were different to the standard North American strain. It is concluded that the genomic and antigenic analysis allowed in the detection of herpetic genital infections caused by herpes virus type I and 2 in the same individual and the identification of herpes virus strains with distinct regional characteristics
Assuntos
Humanos , Feminino , Herpes Genital/genética , Genoma Viral , Herpes Simples/genética , Antígenos Virais/genética , DNA Viral/análise , Herpesvirus Humano 2/genética , Herpesvirus Humano 1/genética , Sorotipagem/métodosRESUMO
A presente revisäo de literatura teve o propósito de estabelecer as correlaçöes existentes entre vários estágios da infecçäo pelo virus herpes simplex com outras patologias discutindo os diversos tratamentos preventivos, profiláticos e terapêuticos existentes, a partir do conhecimento das diversas formas de contágio relacionadas a este tipo de infecçäo