Evaluation of oxidative stability of mayonnaise containing poly ε- caprolactone nanoparticles loaded with thyme essential oil

Suspensions of poly ε-caprolactone (PCL) nanoparticles loaded with thyme essential oil were prepared as a natural antioxidant in mayonnaise. Mean particle size was 204.9 ± 2.7 and 240.0 ± 5.5 nm respectively for nanoparticles prepared with PCL alone (NP-C) and for those loaded with thyme essential oil (NP-T). The polydispersity index indicated a homogeneous distribution of all particles, with no significant difference between NP-C and NP-T samples. The nanoparticles showed a large negative charge evidenced by zeta potential rates, indicating high physical stability. The use of PCL as a polymer provided high encapsulation efficiency for thyme essential oil (91.15 ± 2.12 %). DPPH (2,2-diphenyl-1-picrylhydrazyl) method determined IC50 rates were 476.4 ± 33.6 and 483.5 ± 20.4 µg mL-1respectively for unencapsulated oil and for NP-T, evidencing pronounced antioxidant activity. NP-C, NP-T and synthetic antioxidant butylhydroxytoluene (BHT) were applied to samples of mayonnaise and their oxidative stability evaluated for eight days in an oven at 63 ± 3ºC. Results of hydroperoxide value (HP) and thiobarbituric acid reactive substances (TBARS) showed that NP-T had a similar performance as synthetic antioxidant BHT in the prevention of mayonnaise lipid oxidation

Ensayo de un método de análisis para determinar butilhidroxianisol y butilhidroxitolueno en alimentos / Assay of a method of analysis for butylhydroxyanisole and butylhydroxytoluene in foods

Se ensayó un método de análisis para determinar los contenidos de butilhidroxianisol (BHA) y butilhidroxitolueno (BHT) en alimentos, basado en la extracción con alcohol metílico en una columna que contiene sulfato de sodio anhidro, evaporación del extracto, dilución del residuo con éter etilico e inyección en un cromatógrafo de gases equipado con un detector de ionización de llama. La precisión y la exactitud obtenidas fueron aceptables. El método es útil para investigaciones y para el control sanitario de estos aditivos