Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
1.
Journal of Veterinary Science ; : 379-385, 2004.
Artigo em Inglês | WPRIM | ID: wpr-79775

RESUMO

Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibuthyl phthalate (DBP) on ozone were investigated after 32- and 52-wk exposures using hprt mutation assay. Male and female B6C3F1 mice exposed to ozone (0.5 ppm), NNK (1.0 mg/kg), DBP (5,000 ppm), and two or three combinations of these toxicants 6 h per day for 32- and 52-wk showed increases in the frequencies of TG rlymphocytes compared to the control groups. Additive interactions were noted from two combination groups compared to the ozone alone in both sexes of 32- and 52-wk studies. The most common specific mutation type in the hprt genes of test materials-treated male and female mice was transversion with very few transition. The results indicate that such dominant transversion may be responsible for toxicity and combined exposure to ozone, NNK, and DBP induces additive genotoxicities compared to ozone alone.


Assuntos
Animais , Feminino , Masculino , Camundongos , Carcinógenos/toxicidade , Análise Mutacional de DNA , Dibutilftalato/toxicidade , Combinação de Medicamentos , Hipoxantina Fosforribosiltransferase/genética , Testes de Mutagenicidade , Mutação/efeitos dos fármacos , Nitrosaminas/toxicidade , Ozônio/toxicidade , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Linfócitos T/efeitos dos fármacos
2.
Arq. neuropsiquiatr ; 57(4): 907-11, dez. 1999. tab
Artigo em Inglês | LILACS | ID: lil-249286

RESUMO

The mutation in the hypoxanthine-guanine phosphoribosyltransfere (HPRT) gene has been determined in two brothers affected with Lesch-Nyhan syndrome. Female members of the family who are at risk for being heterozygous carriers of the HPRT mutation were also studied to determine whether they carry the mutation. DNA sequencing revealed that the boys' mother heterozygous for the mutation in her somatic cells, but that three maternal aunts are not heterozygous. Such carrier information is important for the future pregnancy plans of at-risk females. The mutation, an A_T transversion at cDNA base 590 (590 A_T), results in an amino acid change of glutamic acid to valine at codon 197, and has not been reported previously in a Lesch-Nyhan syndrome male. This mutation is designated HPRT.


Assuntos
Adulto , Adolescente , Humanos , Masculino , Feminino , Hipoxantina Fosforribosiltransferase/genética , Síndrome de Lesch-Nyhan/diagnóstico , Brasil , DNA Complementar/análise , População Branca , Heterozigoto , Síndrome de Lesch-Nyhan/genética , Mutação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA