Enzymelinked immunosorbent, immunofluorescence test and electrophoretic analysis of rotaviral RNA in the diagnosis and characterization of the bovine rotavirus

Two handred and ninety five faecal samples from 45 days old calves with and without diarrhoea were processed using the ELISA, PAGE, and IFT techniques for rotavirus detection. Ninety one sample (31%) proved to be positive bu at least one of the techniques used. ELISA detected 93%, PAGE 84% and IFT 67%. Fifteen per cent of the samples were positive using ELISA and negative using PAGE, The reverse situation appeared in 5% of the samples. In 5%, 4% and 1% only of the techniques used showed the presence of the virus (ELISA, PAGE and IFT, respectively). ELISA proved to be the most sensitive technique for the detection of rotavirus in the faeces of calves. No relationship could be established between detection of rotavirus by any one of teh techniques used and age, imptomatology, geographical distribution, and electrophoretic pattern. Rotavirus was detected more frequently in diarrheic than in non-diarrheic calves

Immunologic response of guinea pigs (Cavia porecellus) by treatment with antithymocyte serum, gamma-irradiation of gastrointestinal tract, lymphoid tissue and bone marrow.

The immunologic status of the guinea pigs was modified by treatment with antithymocyte serum (ATS): irradiation of gastrointestinal tract lymphoid tissue (GALT) and bone marrow (BM) by gamma-rays. These animals were injected with homologous testes, kidney and heterologous sheep red blood cells respectively in complete Freund's adjuvant. Cell mediate immune response (CMI) and humoral immune response was studied after first, third, fifth and eighth week of allergization. CMI was completely suppressed in guinea pigs treated with ATS, whereas humoral response suppressed to a lesser extent. In GALT and BM irradiated guinea pigs, CMI develops normally. However, marked suppression in the production of antibody titre both against homologous testes, kidney and heterologous sheep red blood cells was observed. Thymic lymphocytes "T' are destroyed by antithymocyte serum which are the mediators of cell mediated immune response. Irradiation of GALT and BM suppresses humoral response thereby supports that these are populated by "B' lymphocytes.