Non enzymatic glycosylation of alpha-1-proteinase inhibitor of human plasma.

Human plasma contains inhibitors, which control the activity of proteolytic enzymes. Alpha-1-proteinase inhibitor and alpha-2-macroglobulin are two of them present in high concentration in human plasma, which inhibit action of trypsin among other proteinases. The trypsin inhibitory capacity (TIC) of human plasma is observed to be decreased in pathological conditions like diabetes mellitus. The mechanisms of decrease in TIC was due to nonenzymatic glycosylation of alpha-1-proteinase inhibitor (A1PI). A1PI was partially purified from normal human plasma by steps involving ammonium sulphate precipitation, DEAE Sepharose CL6B chromatography, Concanavalin A Sepharose Chromatography and Sephadex G-100 Gel filtration. Purified inhibitor was glycosylated in vitro by incubating it with varying glucose concentrations, under nitrogen for different periods of time in reducing conditions. After glycosylation, the molecular weight of inhibitor increased from 52 kDa to 57 KDa because of binding with glucose molecules. The percent free amino groups in the protein decreased with increasing glucose concentration and days of incubation. The TIC of such modified inhibitor decreased significantly. Decrease in TIC was dependent on the glucose concentration and period of incubation used during in-vitro glycosylation of native inhibitor.

Changes in serum protease inhibitors and liver specific enzymes in experimental jaundice.

Changes in the different fractions of the serum protease inhibitors were studied in experimentally produced cases of obstructive jaundice in rabbits to correlate with other liver specific diagnostic parameters. The heat stable antiprotease fraction and alkaline phosphatase levels in serum were the only parameters which did not show significant fluctuations in the normal as well as in the experimental controls and were significantly elevated due to bile duct ligation. However, due to smaller change in the magnitude, the heat stable antiprotease levels were not found to be of much diagnostic use and the determination of bilirubin, alkaline phosphatase and alanine aminotransferase levels in serum appeared to be better indicators for detection of liver damage in such cases due to appreciable alterations in their levels.

clinical value of serum tumor-associated trypsin inhibitor tati in the diagnosis and follow-up of patients with gynecological tumors

To evaluate the serum levels and clinical value of TATI in different gynecological tumors, this study was carried out including 77 cases [40 with different types of gynecological cancer, 22 with benign diseases and 15 normal healthy controls]. Serum TATI was measured using radioimmunoassay [RIA] kit supplied by Farmos diagnostic. The results of the study showed that serum TATI was highly increased in the cancer group compared to either the group of benign diseases or the normal control group. At 100% specificity, TATI showed a sensitivity of about 60% in gynecological tumors as a whole and 73% in the endometrial, 50% in the ovarian and 60% in the cervical tumors. While at a lower specificity [60%], the sensitivity becomes 75% for the whole cancer patients, 91% in the endometrial, 64% in the ovarian and 60% in the cervical tumors. The benign cases showed a false positivity of about 32%. The follow up of certain cases after surgical interference showed that serum TATI is a valuable marker in monitoring these patients

Pancreatitis aguda: aumento de los inhibidores trípticos en el suero; comunicación previa / Acute pancreatitis: increase of the tryptic inhibitors in the serum: previous communication

Se demuestra el incremento de los inhibidores trípticos en el suero de pacientes con pacreatitis aguda, con una técnica de laboratorio creada por nosotros y por inmunodifusión radial. Se estudia el significado de dicho aumento en la fisiopatología y pronóstico de esta enfermedad