RESUMO
Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype results. Eight cases transformed to AML during follow-up, but no hidden clone was detected by I-FISH in any of them. The inclusion of I-FISH during follow-up of MDS resulted in a small improvement in abnormality detection when compared with conventional G-banding.
Assuntos
Feminino , Humanos , Masculino , Aberrações Cromossômicas , Bandeamento Cromossômico , Hibridização in Situ Fluorescente/métodos , Interfase/genética , Leucemia Mieloide Aguda/genética , Síndromes Mielodisplásicas/genética , Cariotipagem , Estudos ProspectivosRESUMO
Hydroxyurea is considered an antineoplastic drug, which also plays an important role in the treatment of sickle cell anemia patients. We evaluated and compared the clastogenic and cytotoxic effects of hydroxyurea, using chromosomal aberrations and mitotic index, respectively, as endpoints. In vitro short-term cultures of lymphocytes were exposed to several concentrations of this drug, at various cell cycle phases. There was a significant increase in the cytotoxicity of hydroxyurea at G1 and G1/S as well in the G2 phase of the cell cycle. Hydroxyurea did not significantly increase chromosome aberrations. There was an S-dependent cytotoxic effect of hydroxyurea, which is expected based on the known activity of hydroxyurea as an inhibitor of ribonucleotide reductase
Assuntos
Humanos , Aberrações Cromossômicas/induzido quimicamente , Antineoplásicos/toxicidade , Hidroxiureia/toxicidade , Interfase/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Análise de Variância , Determinação de Ponto Final , Fase G1/efeitos dos fármacos , Fase G1/genética , /efeitos dos fármacos , /genética , Fase S/efeitos dos fármacos , Fase S/genética , Interfase/genética , Índice Mitótico , Testes de Mutagenicidade/métodosRESUMO
As the pioneer among molecular cytogenetics techniques, fluorescence in situ hybridization (FISH) allows identification of specific sequences in a structurally preserved cell, in metaphase or interphase. This technique, based on the complementary double-stranded nature of DNA, hybridizes labeled specific DNA (probe). The probe, bound to the target, will be developed into a fluorescent signal. The fact that the signal can be detected clearly, even when fixed in interphase, improves the accuracy of the results, since in some cases it is extremely difficult to obtain mitotic samples. FISH is still used mostly in research, but there are diagnostic applications. New nomenclature is being developed in order to define many of the aberrations that were not distinguished before FISH. Prenatal diagnosis of aneuploidies and malignancies are promptly detected with FISH, which is very useful in critical cases. In some tumors, where chromosomal abnormalities are too complicated to classify manually, the technique of comparative genomic hybridization (CGH), a competitive FISH, allows examiners to determine complete or partial gain or loss of chromosomes. CGH results allow the classification of many tumor cell lines and along with other complementary techniques, like microdissection-FISH, PRINS, etc., increase the possibility of choosing an appropriate treatment for cancer patients
Assuntos
Humanos , Análise Citogenética/métodos , Marcadores Genéticos , Pesquisa em Genética , Neoplasias/genética , Aberrações Cromossômicas , DNA de Neoplasias , Diagnóstico Pré-Natal/métodos , Hibridização in Situ Fluorescente , Interfase/genética , Metáfase/genética , PrognósticoRESUMO
Descrevemos aqui um procedimento rápido para obtençäo de núcleos interfásicos a partir de amostras arquivadas que podem ser utilizados para análise citogenética através da técnica de FISH. Este procedimento difere de outros previamente descritos porque permite a obtençäo de núcleos em número e qualidade satisfatórios sem a utilizaçäo de equipamentos ou lâminas especiais e soluçöes para descondensaçäo da cromatina. O método é de baixo custo e possibilita estudos retrospectivos de tecidos fixados em formol e emblocados em parafina.