Construction and functional analysis of EGFRvIII CAR-T cells co-expressing IL-15 and CCL19 / 生物工程学报

The aim of this study was to investigate the functional characteristics and in vitro specific killing effect of EGFRvIII CAR-T cells co-expressing interleukin-15 and chemokine CCL19, in order to optimize the multiple functions of CAR-T cells and improve the therapeutic effect of CAR-T cells targeting EGFRvIII on glioblastoma (GBM). The recombinant lentivirus plasmid was obtained by genetic engineering, transfected into 293T cells to obtain lentivirus and infected T cells to obtain the fourth generation CAR-T cells targeting EGFRvIII (EGFRvIII-IL-15-CCL19 CAR-T). The expression rate of CAR molecules, proliferation, chemotactic ability, in vitro specific killing ability and anti-apoptotic ability of the fourth and second generation CAR-T cells (EGFRvIII CAR-T) were detected by flow cytometry, cell counter, chemotaxis chamber and apoptosis kit. The results showed that compared with EGFRvIII CAR-T cells, EGFRvIII-IL-15-CCL19 CAR-T cells successfully secreted IL-15 and CCL19, and had stronger proliferation, chemotactic ability and anti-apoptosis ability in vitro (all P < 0.05), while there was no significant difference in killing ability in vitro. Therefore, CAR-T cells targeting EGFRvIII and secreting IL-15 and CCL19 are expected to improve the therapeutic effect of glioblastoma and provide an experimental basis for clinical trials.

effect of gender differences on the response of IL-6, IL-5, cortisol to single session of circuit resistance exercise in elite handball players

Very few studies have been done with regard to the gender differences of the immune system in responding to exercise, which generally do not control women's period cycle. Considering that levels of sex hormones differ during the menstrual cycle this study investigates the influence of gender differences on the response of IL-6, IL-15, cortisol and sex hormone to a single session of circuit resistance exercise in both male and female elite Handball players, while controlling CHECK on the women menstrual period cycles. The participants of the study were 12 elite Handball players, 5 men and 7 women. The circuit resistance exercise included 10 min warming, performing chest press, leg press, leg press, abdominal crunch, literal pull down, leg extension in 3 sets with 10 repetitions with 60% 1RM and 10 min cooling down. Pre, immediately and 2 hours post exercise, the blood samples were collected in order to measure IL-6, IL-15, cortisol, testosterone and estrogen levels. Results indicated that IL-6 concentration was different in the two groups after the exercise, which showed an increase in men and a decrease in women. There was no significant difference between the two groups IL-15 levels, but significant difference was observed between the two groups in cortisol, testosterone and estrogen levels. A single session of circuit resistance exercise resulted in different responses in the Immune system of men, in comparison with women