RESUMO
Abstract Purpose: The effect of a prophylactic oleuropein-rich diet before anesthesia accompanied by the widely-used steroid-based neuromuscular drug rocuronium on mast cell activation was investigated in the study. Methods: 14 rabbits used in the study. The rabbits in the oleuropein group were given oleuropein-rich extract added to the animals' water at doses of 20 mg/kg oleuropein for 15 days orally. After 15 days, all rabbits in the two groups were given general anesthesia with rocuronium of 1 mg/kg. After 1 day, animals were sacrificed and the liver tissue sections stained with H&E, toluidine blue and tryptase for immunohistochemical study. Results: There was no statistically significant difference between ALT, AST and albumin averages of the oleuropein and control groups (p> 0.05). The tryptase average of the control group was higher than the tryptase average of the oleuropein group and this difference was statistically significant (p=0.003). The T. blue average in the oleuropein group was higher than the control group. However, there was no statistically significant difference between groups (p=0.482). Conclusions: Rocuronium adverse effects, like hypersensitivity and anaphylaxis, may limit routine use of this substance. The use of oleuropein reduced the number of inflammatory cells and prevented degranulation.
Assuntos
Animais , Masculino , Coelhos , Fármacos Neuromusculares não Despolarizantes/efeitos adversos , Iridoides/administração & dosagem , Rocurônio/efeitos adversos , Anestesia Geral/efeitos adversos , Mastócitos/efeitos dos fármacos , Anti-Inflamatórios/administração & dosagem , Aspartato Aminotransferases/sangue , Albumina Sérica/análise , Distribuição Aleatória , Degranulação Celular/efeitos dos fármacos , Agregação Celular/efeitos dos fármacos , Reprodutibilidade dos Testes , Cromatografia Líquida de Alta Pressão , Dietoterapia/métodos , Alanina Transaminase/sangue , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/prevenção & controle , Profilaxia Pré-Exposição/métodos , Fígado/efeitos dos fármacos , Fígado/enzimologia , Mastócitos/patologiaRESUMO
The ethanolic extracts, various fractions and two pure compounds isolated from the plant N. arbortris were tested against Encephalomyocarditis Virus (EMCV) and Semliki Forest Virus (SFV). Pronounced in vitro virus inhibitory activity was observed with the ethanolic and n-butanol fractions as well as with the pure compounds arbortristoside A and arbortristoside C. In addition, ethanolic extracts and n-butanol fraction protected EMCV infected mice to the extent of 40 and 60% respectively against SFV at a daily dose of 125 mg/kg body weight.