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1.
Electron. j. biotechnol ; 51: 67-78, May. 2021. graf, tab
Artigo em Inglês | LILACS | ID: biblio-1343435

RESUMO

BACKGROUND: Endometritis is the most common disease of dairy cows and traditionally treated with antibiotics. Lactic acid bacteria can inhibit the growth of pathogens and also have potential for treatment of endometritis. Using PacBio single-molecule real-time sequencing technology, we sequenced the fulllength l6S rRNA of the microbiota in uterine mucus samples from 31 cows with endometritis, treated with lactic acid bacteria (experimental [E] group) and antibiotics (control [C] group) separately. Microbiota profiles taken before and after treatment were compared. RESULTS: After both treatments, bacterial species richness was significantly higher than before, but there was no significant difference in bacterial diversity. Abundance of some bacteria increased after both lactic acid bacteria and antibiotic treatment: Lactobacillus helveticus, Lactococcus lactis, Lactococcus raffinolactis, Pseudomonas alcaligenes and Pseudomonas veronii. The bacterial species that significantly decreased in abundance varied depending on whether the cows had been treated with lactic acid bacteria or antibiotics. Abundance of Staphylococcus equorum and Treponema brennaborense increased after lactic acid bacteria treatment but decreased after antibiotic treatment. According to COG-based functional metagenomic predictions, 384 functional proteins were significantly differently expressed after treatment. E and C group protein expression pathways were significantly higher than before treatment (p < 0.05). CONCLUSIONS: In this study, we found that lactic acid bacteria could cure endometritis and restore a normal physiological state, while avoiding the disadvantages of antibiotic treatment, such as the reductions in abundance of beneficial microbiota. This suggests that lactic acid bacteria treatment has potential as an alternative to antibiotics in the treatment of endometritis in cattle.


Assuntos
Animais , Feminino , Bovinos , Doenças dos Bovinos/tratamento farmacológico , Endometrite/tratamento farmacológico , Lactobacillales/metabolismo , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Antibacterianos/administração & dosagem , Bactérias/isolamento & purificação , Bactérias/crescimento & desenvolvimento , Bactérias/efeitos dos fármacos , Útero/microbiologia , RNA Ribossômico 16S/genética , Ácido Láctico , Lactobacillales/genética , Microbiota
2.
Braz. j. microbiol ; 48(3): 576-586, July-Sept. 2017. tab
Artigo em Inglês | LILACS | ID: biblio-889141

RESUMO

Abstract The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria for production of fermented meat. Lactic acid bacteria isolated from Bulgarian traditional fermented "lulanka" salami was studied regarding some positive technological parameters (growth at different temperature, pH, and proteolytic activity). The presence of genes related to the virulence factors, production of biogenic amines, and vancomycin resistance were presented in low frequency in the studied lactic acid bacteria. On the other hand, production of antimicrobial peptides and high spread of bacteriocin genes were broadly presented. Very strong activity against L. monocytogenes was detected in some of the studied lactic acid bacteria. In addition, the studied strains did not present any antimicrobial activity against tested closely related bacteria such as Lactobacillus spp., Lactococcus spp., Enterococcus spp. or Pediococcus spp. To our knowledge this is the first study on the safety and antimicrobial properties of lactic acid bacteria isolated from Bulgarian lukanka obtained by spontaneous fermentation.


Assuntos
Animais , Lactobacillales/isolamento & purificação , Lactobacillales/metabolismo , Produtos da Carne/microbiologia , Suínos , Aminas Biogênicas/metabolismo , Lactobacillales/efeitos dos fármacos , Lactobacillales/genética , Fermentação , Inocuidade dos Alimentos , Microbiologia de Alimentos , Antibacterianos/farmacologia
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