RESUMO
Objectives: To study expression of glyoxalase I in patients of diabetic retinopathy
Methods: This cross-sectional comparative study was conducted at Centre for Research in Experimental and Applied Medicine [CREAM], Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi in collaboration with Armed Forces Institute of Ophthalmology [AFIO] from January 2015 to November 2015. Sampling technique was non- probability purposive sampling. Total 60 subjects were enrolled in two groups. Group-I comprised 30 patients of diabetic retinopathy and Group-II of 30 normal healthy controls. Clinical and demographic data was collected and fasting venous blood samples [2 ml] were drawn. RNA was extracted and subjected to cDNA synthesis. Expression analysis for glyoxalase I was carried out and relative quantification done by double delta Ct method
Results: Mean age of the patients was 61.30 +/- 7.06 years and mean age of controls was 59.60 +/- 6.43 years. There were 17 [56.7%] males and 13 [43.3%] females in Group-I while Group-II comprised 14 [46.7%] males and 16 [53.3%] females. There was down regulation of glyoxalase I among patients of diabetic retinopathy in comparison with controls when relative gene expression was calculated
Conclusion: Down regulation of glyoxalase I in patients of diabetic retinopathy suggests it to be a contributory factor in the development of disease