1.
Chinese Journal of Biotechnology
;
(12): 932-941, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-826883
RESUMO
Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries. In this study, a new endo-β-N-acetylglucosaminidase, designated as Endo SA, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21 (DE3). The purified recombinant Endo SA exhibited the maximum activity at 35 ºC and pH 6.0, good thermo/pH stability and high specific activity (1.0×10⁶ U/mg). It displayed deglycosylation activity towards different protein substrates. These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.
Assuntos
Clonagem Molecular , Estabilidade Enzimática , Escherichia coli , Genética , Expressão Gênica , Manosil-Glicoproteína Endo-beta-N-Acetilglucosaminidase , Genética , Metabolismo , Proteínas Recombinantes , Genética , Metabolismo , Streptomyces , Genética
2.
Indian J Biochem Biophys
;
1988 Dec; 25(6): 648-54
Artigo
em Inglês
| IMSEAR
| ID: sea-28399